LOCUS       V033324                13110 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033324
VERSION     V033324
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2566
                     /label="MEX3D(NM_203304)"
                     /note="MEX3D(NM_203304)"
                     /gene="MEX3D"
     CDS             2567..2593
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2639..2980
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3106..3605
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3626..4333
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4334..4390
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4391..4990
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5001..5589
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5652..5885
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5910..5928)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6717..6821
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6822..7682
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7853..8441
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8687..9016
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10150..10215
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10345..10365
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10790..10924
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11093..11726
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11773..11898
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12390..12623
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12808..12852
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctaaactt aaggcagtga
      601 actccaagcc accatgccca gctcgctcgg ccagcccgac ggcggcgggg gcgggggcgg
      661 cggcggcggc ggcgtggggg cggcggggga ggaccccgga cccggacctg cgcccccgcc
      721 cgagggcgcc caggaggccg cgcccgcgcc ccggccgccg cccgaacccg acgacgcggc
      781 cgccgcgctc cgcctggcgc tggaccagct gtcggcgctc gggctggggg gcgctggcga
      841 cacggacgag gagggggcgg ccggggacgg cgcagcggcg gcggggggcg cggacggcgg
      901 ggcggctccg gagcctgtgc cccccgacgg acctgaggcc ggcgcgcccc cgaccctggc
      961 ccccgccgtg gcccccgggt cgctgccgct gctggacccc aacgcgagtc ccccgccgcc
     1021 gccgccgccc cggccgtcgc cccccgacgt gttcgcgggc ttcgcgcccc accccgcggc
     1081 cctggggccc ccgacgctgc tggccgacca gatgagcgtg atcggcagcc gcaagaaaag
     1141 cgtcaacatg accgagtgcg tcccggtgcc cagctccgag cacgtcgccg agatcgtggg
     1201 tcgccagggc tgcaagatca aggccctgcg ggccaagaca aacacctaca tcaagacccc
     1261 agtgcggggc gaggagccgg tcttcatcgt gaccggccgg aaggaggacg tggagatggc
     1321 caagcgtgag atcctgtcgg cggccgaaca cttctccatc atccgcgcca cgcgcagcaa
     1381 ggccgggggt ctgcccggcg ccgcccaggg cccgcccaac cttcccggac agaccaccat
     1441 ccaggtgcgc gtgccctacc gggtggtggg gctggtggtg gggcccaagg gcgccaccat
     1501 caagcgcatc cagcagcgga cgcacaccta catcgtgacg cccgggcgcg acaaggagcc
     1561 ggtgttcgcg gtcactggga tgcccgagaa cgtggaccgc gcgcgcgagg agatcgaggc
     1621 gcacatcacg ctgcgcactg gcgccttcac cgacgcgggc cccgacagcg acttccacgc
     1681 caacggcacc gacgtctgcc tggacctgct cggggcggcc gccagcctct gggccaagac
     1741 ccccaaccag ggacgacggc cccccacggc cacggccggc ctccgcgggg acacggccct
     1801 gggcgccccc agcgcccccg aggccttcta cgcgggcagc cgcggcggcc cctccgtgcc
     1861 ggacccaggc cccgccagcc cctacagcgg ctccggcaac gggggcttcg ccttcggcgc
     1921 ggagggtccc ggtgccccgg tggggacggc cgcccccgac gactgcgact tcggcttcga
     1981 cttcgacttc ctggcgctgg acctgaccgt gcccgccgcg gccaccatct gggcgccttt
     2041 tgagcgcgcc gcccccttgc ccgccttcag cggctgctcc acggtcaacg gagccccggg
     2101 acctcccgcc gccggcgccc ggcgcagcag tggggccggg accccccgcc actcgcccac
     2161 gctgcccgag cccggcggcc tccgcctgga gctcccgctg tctcgccgtg gcgccccgga
     2221 cccggtgggc gcgctgtcct ggcgaccccc gcagggcccc gtatccttcc caggcggcgc
     2281 cgccttctcc acggccacct cgctgcccag cagccccgcg gccgccgcct gcgcccccct
     2341 ggactccggc gcctccgaga acagccgcaa gcccccttcg gcgtcctcgg ccccggccct
     2401 ggcgcgagag tgcgtggtgt gcgccgaggg cgaggtgatg gctgcgctgg tcccctgcgg
     2461 ccacaacctc ttctgcatgg actgcgccgt ccgcatctgc ggcaagagcg agcccgagtg
     2521 tcccgcctgc cgcacgccgg ccacccaggc cattcatatc ttttcctacc cctacgacgt
     2581 gcctgactac gccggcggcg gtggttcagg cggtggcggt tccggtggtg ggggatcagt
     2641 ccaactggtg gagtctggtg gcgctttggt gcagccaggt ggctctctgc gtttgtcctg
     2701 tgccgcttct ggcttcccag tgaaccgcta ttccatgcgc tggtatcgcc aggctccagg
     2761 caaagagcgt gagtgggtag ccggtatgtc cagcgcgggt gatcgtagct cctatgaaga
     2821 ctccgtgaag ggccgtttca ccatcagccg tgacgatgcc cgtaacacgg tgtatctgca
     2881 aatgaacagc ttgaaacctg aagatacggc cgtgtattac tgtaatgtga acgtgggctt
     2941 cgagtattgg ggccaaggca cccaggtcac cgtctccagc taagatcctt gacttgcggc
     3001 cgcaactccc acctgcaaca tgcgtgactg actgaggccg cgactctaga gtcgacctgc
     3061 atctaggcgc cggaattaga tctctcgagg ttaacgaatt ctaccgggta ggggaggcgc
     3121 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     3181 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     3241 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     3301 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     3361 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     3421 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     3481 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     3541 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     3601 tttcgacctg cagcccaagc ttaccatggt gagcaagggc gaggaggata acatggccat
     3661 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     3721 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     3781 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     3841 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     3901 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     3961 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     4021 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     4081 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     4141 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     4201 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     4261 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     4321 cgagctgtac aaggcaacaa acttctctct gctgaaacaa gccggagatg tcgaagagaa
     4381 tcctggaccg atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     4441 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     4501 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     4561 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     4621 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     4681 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     4741 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     4801 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     4861 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     4921 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     4981 cggtgcctga tcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     5041 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5101 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5161 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5221 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5281 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5341 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5401 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5461 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5521 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5581 cctccccgca tcgataccgt cgaccaaggc agctgtagat cttagccact ttttaaaaga
     5641 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     5701 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     5761 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     5821 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     5881 tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg
     5941 atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat
     6001 tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga
     6061 gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg
     6121 gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg
     6181 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     6241 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     6301 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     6361 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     6421 aagggagagc gtcgaatggt gcactctcag tacaatctag ctctgatgcc gcatagttaa
     6481 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     6541 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     6601 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     6661 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     6721 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6781 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6841 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6901 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6961 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     7021 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     7081 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     7141 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     7201 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     7261 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     7321 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     7381 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     7441 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7501 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7561 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7621 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7681 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7741 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7801 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7861 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7921 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7981 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8041 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     8101 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8161 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8221 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8281 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8341 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8401 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8461 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8521 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8581 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     8641 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg
     8701 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8761 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8821 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     8881 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8941 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     9001 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
     9061 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
     9121 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
     9181 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
     9241 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
     9301 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga
     9361 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
     9421 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
     9481 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
     9541 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
     9601 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
     9661 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
     9721 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
     9781 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
     9841 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
     9901 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
     9961 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
    10021 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
    10081 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
    10141 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
    10201 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
    10261 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
    10321 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
    10381 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
    10441 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
    10501 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
    10561 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
    10621 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
    10681 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
    10741 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
    10801 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    10861 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    10921 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    10981 taccactgcc aattacctag tggtttcatt tactctaaac ctgtgattcc tctgaattat
    11041 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
    11101 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
    11161 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
    11221 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
    11281 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
    11341 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
    11401 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
    11461 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
    11521 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
    11581 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    11641 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    11701 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
    11761 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
    11821 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
    11881 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
    11941 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
    12001 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
    12061 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
    12121 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
    12181 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
    12241 ggtgatcttc agacctggac gatatatatg agggacaatt ggagaagtga attatataaa
    12301 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
    12361 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
    12421 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
    12481 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
    12541 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
    12601 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
    12661 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
    12721 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
    12781 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
    12841 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
    12901 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
    12961 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
    13021 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
    13081 attcgattag tgaacggatc tcgacggtcg
//
