LOCUS       V033261                12969 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033261
VERSION     V033261
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2425
                     /label="KLHL15(NM_030624)"
                     /note="KLHL15(NM_030624)"
                     /gene="KLHL15"
     CDS             2426..2452
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2498..2839
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2965..3464
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3485..4192
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4193..4249
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4250..4849
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4860..5448
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5511..5744
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5769..5787)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6576..6680
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6681..7541
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7712..8300
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8546..8875
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10009..10074
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10204..10224
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10649..10783
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10952..11585
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11632..11757
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12249..12482
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12667..12711
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgctgga gtagcagtga
      601 actccaagcc accatggcag gggacgtgga aggattctgt tcctccatcc acgacaccag
      661 tgtctctgct gggttcagag cactgtatga ggagggattg cttcttgatg tcactctggt
      721 tattgaagat catcagttcc aggcccataa agcactcttg gccacccaga gtgattactt
      781 cagaattatg tttactgcag acatgaggga acgagatcag gacaaaattc atttaaaagg
      841 tctaacagct accggtttca gccatgtcct gcaatttatg tactatggaa ctatagagct
      901 gagtatgaat accgttcatg agattcttca ggctgccatg tatgttcaac ttatagaagt
      961 ggtgaagttc tgctgctctt ttctcttagc gaagatctgc ctagaaaatt gtgcagaaat
     1021 tatgagactc ttagatgatt tcggcgtaaa catcgaggga gtcagggaga agttagacac
     1081 ctttctgcta gacaactttg tgccactcat gtctaggcct gactttctgt cctatctgag
     1141 ctttgagaag ctcatgtctt acttggataa tgatcatctg agcaggttcc cagagataga
     1201 gctgtacgag gctgtgcagt cttggctgcg gcatgataga agacgctgga gacataccga
     1261 taccatcatt cagaatatcc ggttttgctt gatgacccca accagcgttt ttgagaaggt
     1321 taagacatca gaattttata gatactcccg acagctccgt tacgaagttg accaagcatt
     1381 gaattacttt cagaatgttc accagcagcc tttgttggat atgaagtcaa gccgcatccg
     1441 ttctgcaaaa ccgcaaacta cagtatttcg aggaatgatt ggacatagca tggttaacag
     1501 taaaatactt ctcttaaaga aaccaagagt ctggtgggag ctagaaggcc cacaagtacc
     1561 tctgcgacct gactgccttg ctatcgtcaa taattttgtg ttcctgttag gcggggaaga
     1621 gctgggcccg gatggtgaat tccatgcttc ttccaaagta ttcaggtatg acccgagaca
     1681 gaactcctgg ctgcagatgg cagatatgtc tgtaccacgc tctgaatttg ctgtaggtgt
     1741 tattgggaag tttatttacg ccgtagcagg cagaaccaga gatgagactt tctattcaac
     1801 tgagagatat gacatcacca acgataaatg ggaatttgtg gatccttatc cagttaacaa
     1861 atatggacat gaggggacag tgctcaataa caaattgttt atcaccggtg gaatcacctc
     1921 atcttccacc tccaagcaag tgtgcgtgtt tgaccccagc aaagaaggga ccatagaaca
     1981 acggaccagg agaactcaag tggttaccaa ctgttgggag aataagagca agatgaatta
     2041 cgcgagatgc tttcacaaga tgatttctta caatggcaag ctttatgtct tcggtggtgt
     2101 ctgtgtgatc ttgagggcct ctttcgaatc tcagggatgc ccttctacag aagtatacaa
     2161 cccagagact gatcagtgga ccatcttggc atccatgccg attggtagaa gtggccatgg
     2221 tgtgactgtg ctggacaaac aaataatggt tcttggaggc ctttgttata atggtcatta
     2281 cagcgattcc atcctcactt ttgatccgga tgaaaacaag tggaaggaag atgagtaccc
     2341 tcggatgccc tgcaagctgg atggtttaca agtatgcaac ctgcattttc cggactatgt
     2401 actggatgag gtcaggcgtt gcaactaccc ctacgacgtg cctgactacg ccggcggcgg
     2461 tggttcaggc ggtggcggtt ccggtggtgg gggatcagtc caactggtgg agtctggtgg
     2521 cgctttggtg cagccaggtg gctctctgcg tttgtcctgt gccgcttctg gcttcccagt
     2581 gaaccgctat tccatgcgct ggtatcgcca ggctccaggc aaagagcgtg agtgggtagc
     2641 cggtatgtcc agcgcgggtg atcgtagctc ctatgaagac tccgtgaagg gccgtttcac
     2701 catcagccgt gacgatgccc gtaacacggt gtatctgcaa atgaacagct tgaaacctga
     2761 agatacggcc gtgtattact gtaatgtgaa cgtgggcttc gagtattggg gccaaggcac
     2821 ccaggtcacc gtctccagct aagatccttg acttgcggcc gcaactccca cctgcaacat
     2881 gcgtgactga ctgaggccgc gactctagag tcgacctgca tctaggcgcc ggaattagat
     2941 ctctcgaggt taacgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     3001 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     3061 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     3121 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     3181 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     3241 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     3301 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     3361 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     3421 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct
     3481 taccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     3541 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     3601 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     3661 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     3721 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     3781 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     3841 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     3901 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     3961 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     4021 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     4081 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     4141 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca aggcaacaaa
     4201 cttctctctg ctgaaacaag ccggagatgt cgaagagaat cctggaccga tgaccgagta
     4261 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     4321 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     4381 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     4441 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     4501 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     4561 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     4621 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     4681 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     4741 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     4801 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgat cttatcgata
     4861 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     4921 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     4981 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5041 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5101 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5161 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5221 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     5281 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5341 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5401 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     5461 gaccaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5521 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     5581 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5641 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5701 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt
     5761 ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa
     5821 ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg
     5881 aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc
     5941 ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg
     6001 gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
     6061 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
     6121 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     6181 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     6241 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg
     6301 cactctcagt acaatctagc tctgatgccg catagttaag ccagccccga cacccgccaa
     6361 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     6421 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     6481 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     6541 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6601 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6661 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6721 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6781 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6841 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6901 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6961 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7021 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7081 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7141 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7201 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7261 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7321 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7381 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7441 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7501 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7561 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7621 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7681 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7741 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7801 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     7861 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7921 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7981 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8041 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8101 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8161 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8221 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8281 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8341 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8401 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8461 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     8521 cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     8581 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8641 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8701 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8761 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8821 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     8881 tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga
     8941 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc
     9001 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     9061 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
     9121 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     9181 gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     9241 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     9301 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     9361 tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     9421 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga
     9481 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     9541 acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag
     9601 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     9661 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     9721 cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     9781 gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta
     9841 ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc
     9901 gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt
     9961 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
    10021 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
    10081 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
    10141 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
    10201 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
    10261 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
    10321 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
    10381 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
    10441 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
    10501 ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
    10561 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
    10621 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
    10681 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    10741 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    10801 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctagt
    10861 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    10921 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
    10981 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
    11041 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
    11101 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
    11161 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
    11221 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
    11281 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
    11341 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
    11401 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
    11461 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    11521 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    11581 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
    11641 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
    11701 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
    11761 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
    11821 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
    11881 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
    11941 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
    12001 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
    12061 gagcaaaaca aaagtaagac caccgcacag caagcggccg gtgatcttca gacctggacg
    12121 atatatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
    12181 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
    12241 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
    12301 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
    12361 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
    12421 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
    12481 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
    12541 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
    12601 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
    12661 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
    12721 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
    12781 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    12841 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
    12901 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
    12961 cgacggtcg
//
