LOCUS       V033418                12630 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033418
VERSION     V033418
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2086
                     /label="KCNS3(NM_002252)"
                     /note="KCNS3(NM_002252)"
                     /gene="KCNS3"
     CDS             2087..2113
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2159..2500
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2626..3125
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3146..3853
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3854..3910
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3911..4510
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4521..5109
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5172..5405
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5430..5448)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6237..6341
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6342..7202
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7373..7961
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8207..8536
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9670..9735
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9865..9885
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10310..10444
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10613..11246
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11293..11418
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11910..12143
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12328..12372
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgaggac agcgcagtga
      601 actccaagcc accatggtgt ttggtgagtt tttccatcgc cctggacaag acgaggaact
      661 tgtcaacctg aatgtggggg gctttaagca gtctgttgac caaagcaccc tcctgcggtt
      721 tcctcacacc agactgggga agctgcttac ttgccattct gaagaggcca ttctggagct
      781 gtgtgatgat tacagtgtgg ccgataagga gtactacttt gatcggaatc cctccttgtt
      841 cagatatgtt ttgaattttt attacacggg gaagctgcat gtcatggagg agctgtgcgt
      901 attctcattc tgccaggaga tcgagtactg gggcatcaac gagctcttca ttgattcttg
      961 ctgcagcaat cgctaccagg aacgcaagga ggaaaaccac gagaaggact gggaccagaa
     1021 aagccatgat gtgagtaccg actcctcgtt tgaagagtcg tctctgtttg agaaagagct
     1081 ggagaagttt gacacactgc gatttggtca gctccggaag aaaatctgga ttagaatgga
     1141 gaatccagcg tactgcctgt ccgctaagct tatcgctatc tcctccttga gcgtggtgct
     1201 ggcctccatc gtggccatgt gcgttcacag catgtcggag ttccagaatg aggatggaga
     1261 agtggatgat ccggtgctgg aaggagtgga gatcgcgtgc attgcctggt tcaccgggga
     1321 gcttgccgtc cggctggctg ccgctccttg tcaaaagaaa ttctggaaaa accctctgaa
     1381 catcattgac tttgtctcta ttattccctt ctatgccacg ttggctgtag acaccaagga
     1441 ggaagagagt gaggatattg agaacatggg caaggtggtc cagatcctac ggcttatgag
     1501 gattttccga attctaaagc ttgcccggca ctcggtagga cttcggtctc taggtgccac
     1561 actgagacac agctaccatg aagttgggct tctgcttctc ttcctctctg tgggcatttc
     1621 cattttctct gtgcttatct actccgtgga gaaagatgac cacacatcca gcctcaccag
     1681 catccccatc tgctggtggt gggccaccat cagcatgaca actgtgggct atggagacac
     1741 ccacccggtc accttggcgg gaaagctcat cgccagcaca tgcatcatct gtggcatctt
     1801 ggtggtggcc cttcccatca ccatcatctt caacaagttt tccaagtact accagaagca
     1861 aaaggacatt gatgtggacc agtgcagtga ggatgcacca gagaagtgtc atgagctacc
     1921 ttactttaac attagggata tatatgcaca gcggatgcac accttcatta ccagtctctc
     1981 ttctgtaggc attgtggtga gcgatcctga ctccacagat gcttcaagca ttgaagacaa
     2041 tgaggacatt tgtaacacca cctccttgga gaattgcaca gcaaaatacc cctacgacgt
     2101 gcctgactac gccggcggcg gtggttcagg cggtggcggt tccggtggtg ggggatcagt
     2161 ccaactggtg gagtctggtg gcgctttggt gcagccaggt ggctctctgc gtttgtcctg
     2221 tgccgcttct ggcttcccag tgaaccgcta ttccatgcgc tggtatcgcc aggctccagg
     2281 caaagagcgt gagtgggtag ccggtatgtc cagcgcgggt gatcgtagct cctatgaaga
     2341 ctccgtgaag ggccgtttca ccatcagccg tgacgatgcc cgtaacacgg tgtatctgca
     2401 aatgaacagc ttgaaacctg aagatacggc cgtgtattac tgtaatgtga acgtgggctt
     2461 cgagtattgg ggccaaggca cccaggtcac cgtctccagc taagatcctt gacttgcggc
     2521 cgcaactccc acctgcaaca tgcgtgactg actgaggccg cgactctaga gtcgacctgc
     2581 atctaggcgc cggaattaga tctctcgagg ttaacgaatt ctaccgggta ggggaggcgc
     2641 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     2701 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     2761 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     2821 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     2881 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     2941 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     3001 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     3061 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     3121 tttcgacctg cagcccaagc ttaccatggt gagcaagggc gaggaggata acatggccat
     3181 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     3241 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     3301 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     3361 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     3421 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     3481 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     3541 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     3601 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     3661 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     3721 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     3781 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     3841 cgagctgtac aaggcaacaa acttctctct gctgaaacaa gccggagatg tcgaagagaa
     3901 tcctggaccg atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     3961 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     4021 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     4081 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     4141 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     4201 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     4261 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     4321 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     4381 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     4441 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     4501 cggtgcctga tcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     4561 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4621 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4681 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4741 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4801 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4861 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4921 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4981 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5041 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5101 cctccccgca tcgataccgt cgaccaaggc agctgtagat cttagccact ttttaaaaga
     5161 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     5221 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     5281 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     5341 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     5401 tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg
     5461 atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat
     5521 tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga
     5581 gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg
     5641 gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg
     5701 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     5761 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     5821 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     5881 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     5941 aagggagagc gtcgaatggt gcactctcag tacaatctag ctctgatgcc gcatagttaa
     6001 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     6061 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     6121 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     6181 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     6241 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6301 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6361 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6421 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6481 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6541 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     6601 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6661 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6721 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6781 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6841 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6901 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6961 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7021 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7081 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7141 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7201 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7261 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7321 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7381 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7441 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7501 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7561 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7621 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7681 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7741 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7801 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7861 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7921 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7981 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8041 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8101 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     8161 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg
     8221 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8281 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8341 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     8401 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8461 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8521 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
     8581 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
     8641 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
     8701 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
     8761 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
     8821 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga
     8881 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
     8941 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
     9001 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
     9061 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
     9121 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
     9181 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
     9241 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
     9301 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
     9361 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
     9421 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
     9481 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
     9541 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
     9601 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     9661 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     9721 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     9781 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     9841 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     9901 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     9961 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
    10021 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
    10081 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
    10141 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
    10201 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
    10261 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
    10321 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    10381 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    10441 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    10501 taccactgcc aattacctag tggtttcatt tactctaaac ctgtgattcc tctgaattat
    10561 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
    10621 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
    10681 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
    10741 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
    10801 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
    10861 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
    10921 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
    10981 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
    11041 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
    11101 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    11161 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    11221 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
    11281 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
    11341 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
    11401 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
    11461 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
    11521 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
    11581 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
    11641 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
    11701 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
    11761 ggtgatcttc agacctggac gatatatatg agggacaatt ggagaagtga attatataaa
    11821 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
    11881 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
    11941 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
    12001 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
    12061 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
    12121 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
    12181 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
    12241 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
    12301 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
    12361 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
    12421 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
    12481 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
    12541 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
    12601 attcgattag tgaacggatc tcgacggtcg
//
