LOCUS       V033424                13731 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033424
VERSION     V033424
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..3187
                     /label="KCNB1(NM_004975)"
                     /note="KCNB1(NM_004975)"
                     /gene="KCNB1"
     CDS             3188..3214
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3260..3601
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3727..4226
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4247..4954
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4955..5011
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5012..5611
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5622..6210
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6273..6506
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6531..6549)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7338..7442
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7443..8303
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8474..9062
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9308..9637
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10771..10836
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10966..10986
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11411..11545
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11714..12347
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12394..12519
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    13011..13244
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13429..13473
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttcttatact gtcgcagtga
      601 actccaagcc accatgccgg cgggcatgac gaagcatggc tcccgctcca ccagctcgct
      661 gccgcccgag cccatggaga tcgtgcgcag caaggcgtgc tctcggcggg tccgcctcaa
      721 cgtcgggggg ctggcgcacg aggtactctg gcgtaccctg gaccgcctgc cccgcacgcg
      781 gctgggcaag ctccgcgact gcaacacgca cgactcgctg ctcgaggtgt gcgatgacta
      841 cagcctcgac gacaacgagt acttctttga ccgccacccg ggcgccttca cctccatcct
      901 caacttctac cgcactgggc gactgcacat gatggaggag atgtgcgcgc tcagcttcag
      961 ccaagagctc gactactggg gcatcgacga gatctacctg gagtcctgct gccaggcccg
     1021 ctaccaccag aagaaagagc agatgaacga ggagctcaag cgtgaggccg agaccctacg
     1081 ggagcgggaa ggcgaggagt tcgataacac gtgctgcgca gagaagagga aaaaactctg
     1141 ggacctactg gagaagccca attcctctgt ggctgccaag atccttgcca taatttccat
     1201 catgttcatc gtcctctcca ccattgccct gtccctcaac acgctgcctg agctacagag
     1261 cctcgatgag ttcggccagt ccacagacaa cccccagctg gcccacgtgg aggccgtgtg
     1321 catcgcatgg ttcaccatgg agtacctgct gaggttcctc tcctcgccca agaagtggaa
     1381 gttcttcaag ggcccactca atgccattga cttgttggcc attctgccat actatgtcac
     1441 cattttcctc accgaatcca acaagagcgt gctgcaattc cagaatgtcc gccgcgtggt
     1501 ccagatcttc cgcatcatgc gaattctccg catccttaag cttgcacgcc actccactgg
     1561 cctccagtct ctgggcttca ctttgcggag gagctacaat gagttgggct tgctcatcct
     1621 cttccttgcc atgggcatta tgatcttctc cagccttgtc ttctttgctg agaaggatga
     1681 ggacgacacc aagttcaaaa gcatcccagc ctctttctgg tgggccacca tcaccatgac
     1741 tactgttggg tatggagaca tctaccccaa gactctcctg gggaaaattg ttgggggact
     1801 ctgctgcatt gcaggagtcc tggtgattgc tcttcccatc cccatcatcg tcaataactt
     1861 ctctgagttc tataaggagc agaagagaca ggagaaagca atcaaacggc gagaggctct
     1921 ggagagagcc aagaggaatg gcagcatcgt atccatgaac atgaaggatg cttttgcccg
     1981 gagcattgag atgatggaca ttgtggttga gaaaaatggg gagaatatgg gtaagaaaga
     2041 caaagtacaa gataaccact tgtctcctaa caaatggaaa tggacaaaga ggacactgtc
     2101 tgaaaccagc tcaagtaagt cctttgaaac caaggaacag ggatcccctg aaaaagccag
     2161 atcgtcttct agtcctcagc acctgaacgt tcagcagttg gaagacatgt acaataagat
     2221 ggccaagacc caatcccaac ccatcctcaa taccaaggag tcagcagcac agagcaaacc
     2281 aaaggaagaa cttgaaatgg agagtatccc cagccccgta gcccctctgc ccactcgcac
     2341 agaaggggtc attgacatgc gaagtatgtc aagcattgat agtttcatta gctgtgccac
     2401 agacttccct gaggccacca gattctccca cagccctttg acatcactcc ccagcaagac
     2461 tgggggcagc acagccccag aagtgggctg gcggggagct ctgggtgcca gtggtggtag
     2521 gtttgtggag gccaacccca gccctgatgc cagccagcac tctagtttct tcatcgagag
     2581 ccccaagagt tccatgaaaa ctaacaaccc tttgaagctc cgagcactta aagtcaactt
     2641 catggagggt gaccccagtc cactcctccc cgttctaggg atgtaccatg accctctcag
     2701 gaaccggggg agtgctgcgg ctgctgtcgc tggactggag tgtgccacgc ttttggacaa
     2761 ggctgtgctg agcccagagt cctccatcta caccacagca agtgctaaga cacccccccg
     2821 gtctcctgag aaacacacag caatagcgtt caactttgag gcgggtgtcc accagtacat
     2881 tgacgcagac acagatgatg agggacagct gctctacagt gtggactcca gcccccccaa
     2941 aagcctccct gggagcacca gtccgaagtt cagcacgggg acaagatcgg agaaaaacca
     3001 ctttgaaagc tcccctttac ccacctcccc taagttctta aggcagaact gtatttactc
     3061 cacagaagca ttgactggaa aaggccccag tggtcaggaa aagtgcaaac ttgagaacca
     3121 catctcccct gacgtccgtg tgttgccagg gggaggagcc catggaagca cacgagatca
     3181 gagcatctac ccctacgacg tgcctgacta cgccggcggc ggtggttcag gcggtggcgg
     3241 ttccggtggt gggggatcag tccaactggt ggagtctggt ggcgctttgg tgcagccagg
     3301 tggctctctg cgtttgtcct gtgccgcttc tggcttccca gtgaaccgct attccatgcg
     3361 ctggtatcgc caggctccag gcaaagagcg tgagtgggta gccggtatgt ccagcgcggg
     3421 tgatcgtagc tcctatgaag actccgtgaa gggccgtttc accatcagcc gtgacgatgc
     3481 ccgtaacacg gtgtatctgc aaatgaacag cttgaaacct gaagatacgg ccgtgtatta
     3541 ctgtaatgtg aacgtgggct tcgagtattg gggccaaggc acccaggtca ccgtctccag
     3601 ctaagatcct tgacttgcgg ccgcaactcc cacctgcaac atgcgtgact gactgaggcc
     3661 gcgactctag agtcgacctg catctaggcg ccggaattag atctctcgag gttaacgaat
     3721 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     3781 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     3841 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     3901 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     3961 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     4021 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     4081 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     4141 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     4201 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatgg tgagcaaggg
     4261 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     4321 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     4381 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     4441 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     4501 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     4561 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     4621 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     4681 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     4741 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     4801 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     4861 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     4921 ccactccacc ggcggcatgg acgagctgta caaggcaaca aacttctctc tgctgaaaca
     4981 agccggagat gtcgaagaga atcctggacc gatgaccgag tacaagccca cggtgcgcct
     5041 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     5101 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     5161 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     5221 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     5281 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     5341 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     5401 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     5461 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     5521 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     5581 ctggtgcatg acccgcaagc ccggtgcctg atcttatcga taatcaacct ctggattaca
     5641 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     5701 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     5761 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5821 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5881 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5941 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6001 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     6061 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6121 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     6181 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgaccaagg cagctgtaga
     6241 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     6301 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     6361 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6421 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6481 ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt
     6541 cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac
     6601 gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct
     6661 ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac
     6721 gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc
     6781 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     6841 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     6901 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     6961 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     7021 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatcta
     7081 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     7141 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     7201 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     7261 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     7321 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7381 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7441 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7501 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7561 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7621 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7681 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7741 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7801 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7861 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7921 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7981 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8041 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8101 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8161 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8221 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8281 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8341 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8401 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8461 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8521 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8581 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     8641 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8701 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8761 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8821 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8881 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8941 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9001 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9061 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9241 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     9301 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9361 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     9421 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     9481 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9541 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     9601 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt
     9661 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     9721 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     9781 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     9841 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     9901 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc
     9961 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
    10021 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
    10081 aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga
    10141 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
    10201 tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg
    10261 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg
    10321 cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg
    10381 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
    10441 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc
    10501 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
    10561 gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg
    10621 aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt
    10681 atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
    10741 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
    10801 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
    10861 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
    10921 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
    10981 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
    11041 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
    11101 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
    11161 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
    11221 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
    11281 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
    11341 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
    11401 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    11461 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    11521 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
    11581 acttcccacc ccatacccta ttaccactgc caattaccta gtggtttcat ttactctaaa
    11641 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    11701 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
    11761 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
    11821 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
    11881 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
    11941 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
    12001 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
    12061 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
    12121 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
    12181 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    12241 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    12301 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
    12361 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
    12421 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
    12481 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
    12541 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
    12601 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
    12661 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
    12721 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
    12781 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
    12841 accaccgcac agcaagcggc cggtgatctt cagacctgga cgatatatat gagggacaat
    12901 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
    12961 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
    13021 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
    13081 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
    13141 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
    13201 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
    13261 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
    13321 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
    13381 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
    13441 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
    13501 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
    13561 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
    13621 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
    13681 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggtc g
//
