LOCUS       V030074                13686 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030074
VERSION     V030074
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..3142
                     /label="FBXO11(NM_025133)"
                     /note="FBXO11(NM_025133)"
                     /gene="FBXO11"
     CDS             3143..3169
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3215..3556
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3682..4181
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4202..4909
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4910..4966
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4967..5566
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5577..6165
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6228..6461
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6486..6504)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7293..7397
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7398..8258
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8429..9017
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9263..9592
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10726..10791
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10921..10941
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11366..11500
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11669..12302
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12349..12474
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12966..13199
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13384..13428
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgcgttt ttggcagtga
      601 actccaagcc accatggttg cagaagaatc aggtcctggt gcacaaaata gtccatacca
      661 acttcgtaga aaaactcttt tgccgaaaag aacagcgtgt cccacaaaga acagtatgga
      721 gggcgcctca acttcaacta cagaaaactt tggtcatcgt gcaaaacgtg caagagtgtc
      781 tggaaaatca caagatctat cagcagcacc tgctgaacag tatcttcagg agaaactgcc
      841 agatgaagtg gttctaaaaa tcttctctta cttgctggaa caggatcttt gtagagcagc
      901 ttgtgtatgt aaacgcttca gtgaacttgc taatgatcca attttgtgga aacgattata
      961 tatggaagta tttgaatata ctcgccctat gatgcatcct gaacctggaa aattctacca
     1021 gattaatcca gaagagtatg aacatccaaa tccctggaaa gagagtttcc agcagttgta
     1081 taaaggtgca catgtaaagc caggatttgc tgaacatttc tacagtaacc ctgcaagata
     1141 taaaggaaga gaaaatatgt tgtattatga tactattgaa gatgcccttg gtggggtaca
     1201 agaggctcat tttgatggac ttatctttgt tcattctgga atatatactg atgaatggat
     1261 atatattgaa tctccaatca ccatgattgg tgcagcacct gggaaagtgg cagacaaagt
     1321 tataattgaa aacactagag attcaacctt cgtttttatg gaaggctctg aagatgctta
     1381 tgttggatat atgacaataa ggtttaaccc tgatgacaaa tctgcacaac accacaatgc
     1441 acaccactgc ttagagatta cagtaaattg tagccctatt attgatcact gtatcatccg
     1501 aagtacatgt acagttggtt ctgcagtatg tgttagtggt caaggagcat gtcccaccat
     1561 caagcactgt aacatcagtg actgtgaaaa tgttggacta tatataacag atcatgcaca
     1621 gggaatatat gaggataatg aaatttccaa taatgcgtta gctgggattt gggttaaaaa
     1681 tcatggaaac ccaattatta gacggaatca tattcatcat ggacgtgatg ttggtgtgtt
     1741 cacatttgat catggcatgg gttactttga aagttgcaat atacacagaa ataggatagc
     1801 aggctttgaa gtaaaagcct atgctaaccc tacagtggtt cgatgtgaaa ttcaccatgg
     1861 gcagactgga ggaatatatg tccatgaaaa aggaagagga caattcatag agaataaaat
     1921 ctatgcaaac aactttgcag gtgtatggat tacctcaaat agtgacccaa caataagggg
     1981 aaattctata tttaatggaa atcaaggagg agtttacatc tttggtgatg gacgaggcct
     2041 tattgaagga aatgacattt atggcaatgc attagcagga attcaaatta ggacaaacag
     2101 ttgtccaatt gttcggcata acaaaattca tgatggccag catggtggga tttatgtgca
     2161 tgaaaaggga caaggagtaa tagaagagaa tgaagtttat agtaacactc tagctggagt
     2221 ctgggtgaca actggcagca ctccagtact gagaagaaac cggatacaca gtggcaagca
     2281 ggttggtgtt tatttttatg acaatggaca tggagtgcta gaagacaatg atatctataa
     2341 tcatatgtat tcaggggttc agataaggac tggaagcaac cccaaaatta gacgcaacaa
     2401 aatctgggga ggacagaatg gtggaattct agtttataat tctggtctag gctgtataga
     2461 agacaatgaa atatttgaca atgcaatggc tggagtctgg attaagacag atagtaatcc
     2521 tacactaaga agaaataaaa tccatgatgg aagagatggt ggcatctgta tatttaatgg
     2581 gggtcgaggt ctccttgaag aaaatgatat tttcaggaat gctcaagcag gtgttctcat
     2641 cagcactaat agtcatccaa tcttaaggaa aaacagaata tttgatggat ttgccgcagg
     2701 tattgaaatt acaaatcacg caactgcaac actagaaggc aatcagattt ttaacaaccg
     2761 gtttggaggc ttatttttag catctggtgt taatgtgaca atgaaagata acaaaataat
     2821 gaacaatcaa gatgccatag aaaaggctgt tagtagaggc caatgtttat ataaaatatc
     2881 aagttatacc agctatccca tgcatgattt ctacagatgt catacttgta acaccacaga
     2941 tcgaaatgcc atatgtgtga actgcattaa gaagtgccat cagggacatg atgtagagtt
     3001 tattagacat gataggtttt tctgtgactg tggtgctgga acactgtcta atccttgtac
     3061 attagctggt gagcctacac atgatacaga tacactatat gactctgctc cacctataga
     3121 atctaataca ttgcagcaca actaccccta cgacgtgcct gactacgccg gcggcggtgg
     3181 ttcaggcggt ggcggttccg gtggtggggg atcagtccaa ctggtggagt ctggtggcgc
     3241 tttggtgcag ccaggtggct ctctgcgttt gtcctgtgcc gcttctggct tcccagtgaa
     3301 ccgctattcc atgcgctggt atcgccaggc tccaggcaaa gagcgtgagt gggtagccgg
     3361 tatgtccagc gcgggtgatc gtagctccta tgaagactcc gtgaagggcc gtttcaccat
     3421 cagccgtgac gatgcccgta acacggtgta tctgcaaatg aacagcttga aacctgaaga
     3481 tacggccgtg tattactgta atgtgaacgt gggcttcgag tattggggcc aaggcaccca
     3541 ggtcaccgtc tccagctaag atccttgact tgcggccgca actcccacct gcaacatgcg
     3601 tgactgactg aggccgcgac tctagagtcg acctgcatct aggcgccgga attagatctc
     3661 tcgaggttaa cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     3721 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     3781 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     3841 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     3901 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     3961 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     4021 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     4081 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     4141 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     4201 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     4261 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     4321 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     4381 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     4441 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     4501 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     4561 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     4621 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     4681 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     4741 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     4801 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     4861 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg caacaaactt
     4921 ctctctgctg aaacaagccg gagatgtcga agagaatcct ggaccgatga ccgagtacaa
     4981 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     5041 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     5101 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     5161 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     5221 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     5281 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     5341 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     5401 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     5461 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     5521 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgatctt atcgataatc
     5581 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5641 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5701 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5761 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5821 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     5881 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5941 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     6001 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6061 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     6121 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     6181 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     6241 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     6301 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6361 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6421 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
     6481 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
     6541 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
     6601 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
     6661 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
     6721 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     6781 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     6841 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     6901 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     6961 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
     7021 tctcagtaca atctagctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     7081 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     7141 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     7201 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     7261 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7321 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7381 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7441 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7501 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7561 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7621 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7681 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7741 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7801 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7861 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7921 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7981 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8041 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8101 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8161 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8221 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8281 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8341 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8401 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8461 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8521 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     8581 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8641 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8701 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8761 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8821 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8881 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8941 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9001 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9061 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9121 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9181 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9241 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     9301 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     9361 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     9421 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9481 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     9541 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
     9601 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
     9661 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
     9721 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
     9781 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
     9841 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
     9901 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     9961 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
    10021 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
    10081 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
    10141 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
    10201 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
    10261 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
    10321 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
    10381 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
    10441 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
    10501 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
    10561 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
    10621 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
    10681 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
    10741 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
    10801 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
    10861 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
    10921 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
    10981 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
    11041 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
    11101 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
    11161 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
    11221 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
    11281 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
    11341 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
    11401 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    11461 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    11521 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctagtggt
    11581 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    11641 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
    11701 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
    11761 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
    11821 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
    11881 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
    11941 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
    12001 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
    12061 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
    12121 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    12181 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    12241 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    12301 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
    12361 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
    12421 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    12481 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    12541 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    12601 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    12661 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    12721 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    12781 caaaacaaaa gtaagaccac cgcacagcaa gcggccggtg atcttcagac ctggacgata
    12841 tatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
    12901 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
    12961 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
    13021 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
    13081 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    13141 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    13201 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    13261 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    13321 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    13381 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    13441 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    13501 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    13561 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    13621 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
    13681 cggtcg
//
