LOCUS       V033294                13020 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033294
VERSION     V033294
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2476
                     /label="FBXL4(NM_012160)"
                     /note="FBXL4(NM_012160)"
                     /gene="FBXL4"
     CDS             2477..2503
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2549..2890
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3016..3515
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3536..4243
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4244..4300
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4301..4900
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4911..5499
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5562..5795
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5820..5838)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6627..6731
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6732..7592
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7763..8351
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8597..8926
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10060..10125
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10255..10275
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10700..10834
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11003..11636
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11683..11808
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12300..12533
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12718..12762
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctatattt gtagcagtga
      601 actccaagcc accatgtcac cggtctttcc catgttaaca gttctgacca tgttttatta
      661 tatatgcctt cggcgccgag ccaggacagc tacaagagga gaaatgatga acacccatag
      721 agctatagaa tcaaacagcc agacttcccc tctcaatgca gaggtagtcc agtatgccaa
      781 agaagtagtg gatttcagtt cccattatgg aagtgagaat agtatgtcct atactatgtg
      841 gaatttggct ggtgtaccaa atgtattccc aagttctggt gactttactc agacagctgt
      901 gtttcgaact tatgggacat ggtgggatca gtgtcctagt gcttccttgc cattcaagag
      961 gacgccacct aattttcaga gccaggacta tgtggaactt acttttgaac aacaggtgta
     1021 tcctacagct gtacatgttc tagaaaccta tcatcccgga gcagtcatta gaattctcgc
     1081 ttgttctgca aatccttatt ccccaaatcc accagctgaa gtaagatggg agattctttg
     1141 gtcagagaga cctacgaagg tgaatgcttc ccaagctcgc cagtttaaac cttgtattaa
     1201 gcagataaat ttccccacaa atcttatacg actggaagta aatagttctc ttctggaata
     1261 ttacactgaa ttagatgcag ttgtgctaca tggtgtgaag gacaagccag tgctttctct
     1321 caagacttca cttattgaca tgaatgatat agaagatgat gcctatgcag aaaaggatgg
     1381 ttgtggaatg gacagtctta acaaaaagtt tagcagtgct gtcctcgggg aagggccaaa
     1441 taatgggtat tttgataaac taccttatga gcttattcag ctgattctga atcatcttac
     1501 actaccagac ctgtgtagat tagcacagac ttgcaaacta ctgagccagc attgctgtga
     1561 tcctctgcaa tacatccacc tcaatctgca accatactgg gcaaaactag atgacacttc
     1621 tctggaattt ctacagtctc gctgcactct tgtccagtgg cttaatttat cttggactgg
     1681 caatagaggc ttcatctctg ttgcaggatt tagcaggttt ctgaaggttt gtggatccga
     1741 attagtacgc cttgaattgt cttgcagcca ctttcttaat gaaacttgct tagaagttat
     1801 ttctgagatg tgtccaaatc tacaggcctt aaatctctcc tcctgtgata agctaccacc
     1861 tcaagctttc aaccacattg ccaagttatg cagccttaaa cgacttgttc tctatcgaac
     1921 aaaagtagag caaacagcac tgctcagcat tttgaacttc tgttcagagc ttcagcacct
     1981 cagtttaggc agttgtgtca tgattgaaga ctatgatgtg atagctagca tgataggagc
     2041 caagtgtaaa aaactccgga ccctggatct gtggagatgt aagaatatta ctgagaatgg
     2101 aatagcagaa ctggcttctg ggtgtccact actggaggag cttgaccttg gctggtgccc
     2161 aactctgcag agcagcaccg ggtgcttcac cagactggca caccagctcc caaacttgca
     2221 aaaactcttt cttacagcta atagatctgt gtgtgacaca gacattgatg aattggcatg
     2281 taattgtacc aggttacagc agctggacat attaggaaca agaatggtaa gtccggcatc
     2341 cttaagaaaa ctcctggaat cttgtaaaga tctttcttta cttgatgtgt ccttctgttc
     2401 gcagattgat aacagagctg tgctagaact gaatgcaagc tttccaaaag tgttcataaa
     2461 aaagagcttt actcagtacc cctacgacgt gcctgactac gccggcggcg gtggttcagg
     2521 cggtggcggt tccggtggtg ggggatcagt ccaactggtg gagtctggtg gcgctttggt
     2581 gcagccaggt ggctctctgc gtttgtcctg tgccgcttct ggcttcccag tgaaccgcta
     2641 ttccatgcgc tggtatcgcc aggctccagg caaagagcgt gagtgggtag ccggtatgtc
     2701 cagcgcgggt gatcgtagct cctatgaaga ctccgtgaag ggccgtttca ccatcagccg
     2761 tgacgatgcc cgtaacacgg tgtatctgca aatgaacagc ttgaaacctg aagatacggc
     2821 cgtgtattac tgtaatgtga acgtgggctt cgagtattgg ggccaaggca cccaggtcac
     2881 cgtctccagc taagatcctt gacttgcggc cgcaactccc acctgcaaca tgcgtgactg
     2941 actgaggccg cgactctaga gtcgacctgc atctaggcgc cggaattaga tctctcgagg
     3001 ttaacgaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     3061 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     3121 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     3181 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     3241 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     3301 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     3361 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     3421 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     3481 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatggt
     3541 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     3601 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3661 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     3721 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     3781 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     3841 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     3901 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     3961 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     4021 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     4081 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     4141 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     4201 cgagggccgc cactccaccg gcggcatgga cgagctgtac aaggcaacaa acttctctct
     4261 gctgaaacaa gccggagatg tcgaagagaa tcctggaccg atgaccgagt acaagcccac
     4321 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     4381 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     4441 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     4501 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     4561 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     4621 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     4681 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     4741 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     4801 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     4861 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga tcttatcgat aatcaacctc
     4921 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4981 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5041 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5101 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5161 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5221 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5281 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     5341 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5401 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     5461 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgaccaaggc
     5521 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5581 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     5641 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5701 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5761 cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt
     5821 ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg
     5881 cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc
     5941 ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt
     6001 tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct
     6061 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     6121 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     6181 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     6241 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     6301 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag
     6361 tacaatctag ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     6421 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     6481 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     6541 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     6601 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6661 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6721 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6781 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6841 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6901 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6961 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7021 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7081 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7141 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7201 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7261 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7321 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7381 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7441 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7501 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7561 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7621 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7681 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7741 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7801 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7861 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     7921 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7981 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8041 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8101 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8161 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8221 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8281 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8341 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8401 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8461 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8521 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     8581 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     8641 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     8701 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     8761 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8821 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8881 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
     8941 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
     9001 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
     9061 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
     9121 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
     9181 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
     9241 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
     9301 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
     9361 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
     9421 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
     9481 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
     9541 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
     9601 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
     9661 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
     9721 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
     9781 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
     9841 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
     9901 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
     9961 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
    10021 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
    10081 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
    10141 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
    10201 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
    10261 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
    10321 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
    10381 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
    10441 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
    10501 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
    10561 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
    10621 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    10681 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    10741 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    10801 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
    10861 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctag tggtttcatt
    10921 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    10981 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
    11041 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
    11101 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
    11161 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
    11221 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
    11281 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
    11341 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
    11401 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
    11461 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
    11521 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
    11581 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
    11641 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
    11701 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
    11761 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
    11821 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
    11881 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
    11941 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
    12001 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
    12061 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
    12121 aaaagtaaga ccaccgcaca gcaagcggcc ggtgatcttc agacctggac gatatatatg
    12181 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
    12241 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
    12301 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
    12361 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
    12421 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
    12481 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
    12541 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
    12601 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
    12661 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
    12721 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
    12781 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
    12841 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
    12901 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
    12961 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtcg
//
