LOCUS       V033279                12039 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033279
VERSION     V033279
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1495
                     /label="ASB9(NM_001031739)"
                     /note="ASB9(NM_001031739)"
                     /gene="ASB9"
     CDS             1496..1522
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1568..1909
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2035..2534
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             2555..3262
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3263..3319
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3320..3919
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    3930..4518
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             4581..4814
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(4839..4857)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        5646..5750
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             5751..6611
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      6782..7370
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        7616..7945
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9079..9144
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9274..9294
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    9719..9853
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10022..10655
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    10702..10827
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11319..11552
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             11737..11781
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttcttaaaac tcggcagtga
      601 actccaagcc accatggatg gcaaacaagg gggcatggat gggagcaagc ccgcggggcc
      661 aagggacttt cctggcatca ggcttctttc aaacccattg atgggcgatg ctgtgtctga
      721 ttggtctcct atgcatgaag ctgcaatcca cggacatcag ctgtctctga ggaacctcat
      781 cagccagggg tgggctgtga acatcatcac ggcagatcat gtttccccac tccatgaagc
      841 ctgtcttgga ggtcatctct cttgtgtgaa gattttatta aagcatggag ctcaggtgaa
      901 tggtgtgaca gcagactggc acactccact gtttaatgct tgtgtcagcg gcagctggga
      961 ttgtgtgaat ttgcttctgc agcacggagc cagcgttcaa cctgagagtg atctggcatc
     1021 ccccatccat gaagctgcta ggagaggcca cgtggagtgt gtcaactctc ttatagctta
     1081 tgggggcaac attgaccata agatcagcca cctgggcact ccactctatt tggcttgtga
     1141 aaaccaacag agagcctgtg tcaagaagct tctggagtca ggagcggacg tgaaccaagg
     1201 gaaaggtcag gattccccac ttcatgcagt ggccaggaca gccagtgaag agctggcctg
     1261 cctgctcatg gattttggag cggacaccca ggccaagaat gctgaaggca aacgtcctgt
     1321 ggagctggtg cctccagaga gccccttggc ccagctcttc ttggagagag aagggccccc
     1381 ttctttgatg cagttatgcc gccttagaat tcggaagtgt tttggaatcc agcagcatca
     1441 taagataacc aaactcgtcc tcccagagga tctgaaacag tttctcctac atctttaccc
     1501 ctacgacgtg cctgactacg ccggcggcgg tggttcaggc ggtggcggtt ccggtggtgg
     1561 gggatcagtc caactggtgg agtctggtgg cgctttggtg cagccaggtg gctctctgcg
     1621 tttgtcctgt gccgcttctg gcttcccagt gaaccgctat tccatgcgct ggtatcgcca
     1681 ggctccaggc aaagagcgtg agtgggtagc cggtatgtcc agcgcgggtg atcgtagctc
     1741 ctatgaagac tccgtgaagg gccgtttcac catcagccgt gacgatgccc gtaacacggt
     1801 gtatctgcaa atgaacagct tgaaacctga agatacggcc gtgtattact gtaatgtgaa
     1861 cgtgggcttc gagtattggg gccaaggcac ccaggtcacc gtctccagct aagatccttg
     1921 acttgcggcc gcaactccca cctgcaacat gcgtgactga ctgaggccgc gactctagag
     1981 tcgacctgca tctaggcgcc ggaattagat ctctcgaggt taacgaattc taccgggtag
     2041 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     2101 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     2161 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     2221 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     2281 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     2341 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     2401 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     2461 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     2521 ctccgggcct ttcgacctgc agcccaagct taccatggtg agcaagggcg aggaggataa
     2581 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     2641 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     2701 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     2761 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     2821 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     2881 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     2941 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     3001 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     3061 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     3121 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     3181 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     3241 cggcatggac gagctgtaca aggcaacaaa cttctctctg ctgaaacaag ccggagatgt
     3301 cgaagagaat cctggaccga tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     3361 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     3421 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     3481 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     3541 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     3601 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     3661 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     3721 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     3781 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     3841 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     3901 ccgcaagccc ggtgcctgat cttatcgata atcaacctct ggattacaaa atttgtgaaa
     3961 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     4021 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     4081 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     4141 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4201 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4261 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4321 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     4381 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     4441 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     4501 tttgggccgc ctccccgcat cgataccgtc gaccaaggca gctgtagatc ttagccactt
     4561 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     4621 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     4681 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     4741 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     4801 gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt
     4861 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac
     4921 aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact
     4981 gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac
     5041 ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga
     5101 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     5161 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     5221 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     5281 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     5341 gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctagc tctgatgccg
     5401 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     5461 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     5521 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     5581 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     5641 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5701 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     5761 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5821 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5881 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5941 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     6001 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     6061 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     6121 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     6181 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     6241 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     6301 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     6361 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     6421 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     6481 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     6541 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     6601 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     6661 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6721 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6781 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6841 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6901 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     6961 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7021 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7081 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7141 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7201 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7261 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7321 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7381 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     7441 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     7501 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     7561 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
     7621 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7681 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7741 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     7801 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7861 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7921 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
     7981 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
     8041 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
     8101 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
     8161 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
     8221 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
     8281 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
     8341 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
     8401 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
     8461 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
     8521 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
     8581 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
     8641 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
     8701 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
     8761 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
     8821 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
     8881 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
     8941 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
     9001 cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
     9061 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     9121 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     9181 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     9241 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     9301 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     9361 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     9421 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     9481 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     9541 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     9601 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     9661 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     9721 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     9781 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9841 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
     9901 ataccctatt accactgcca attacctagt ggtttcattt actctaaacc tgtgattcct
     9961 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    10021 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
    10081 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
    10141 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
    10201 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
    10261 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
    10321 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
    10381 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
    10441 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
    10501 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    10561 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    10621 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
    10681 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
    10741 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
    10801 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
    10861 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
    10921 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
    10981 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
    11041 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
    11101 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
    11161 caagcggccg gtgatcttca gacctggacg atatatatga gggacaattg gagaagtgaa
    11221 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
    11281 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
    11341 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
    11401 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
    11461 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
    11521 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
    11581 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
    11641 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
    11701 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    11761 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    11821 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    11881 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    11941 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    12001 gacagatcca ttcgattagt gaacggatct cgacggtcg
//
