LOCUS       V028354                11820 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028354
VERSION     V028354
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        3..229
                     /note="Rous sarcoma virus enhancer/promoter"
                     /label="RSV promoter"
     LTR             230..410
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    457..582
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1075..1308
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1803..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    1938..1958
                     /bound_moiety="BP Clonase™"
                     /gene="mutant version of attB"
                     /note="core recombination site for the Gateway® BP
                     reaction"
                     /label="attB4"
     enhancer        2019..2322
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2323..2526
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2566..6489
                     /label="ERBB4(NM_005235)"
                     /note="ERBB4(NM_005235)"
                     /gene="ERBB4"
     misc_feature    6535..7123
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     promoter        7154..7653
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             7670..8269
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     LTR             8341..8574
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8646..8767
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8807..8942
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        complement(8963..8981)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(8991..9007)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     rep_origin      9149..9604
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9630..9734
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             9735..10595
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      10766..11354
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    11642..11663
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        11678..11708
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11716..11732
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     11740..11756
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        11777..11795
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgta gttattaata gtaatcaatt
     1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2521 agagctggtt tagtgaaccg tcagatccaa gttggatccg ccaccatgaa gccggcgaca
     2581 ggactttggg tctgggtgag ccttctcgtg gcggcgggga ccgtccagcc cagcgattct
     2641 cagtcagtgt gtgcaggaac ggagaataaa ctgagctctc tctctgacct ggaacagcag
     2701 taccgagcct tgcgcaagta ctatgaaaac tgtgaggttg tcatgggcaa cctggagata
     2761 accagcattg agcacaaccg ggacctctcc ttcctgcggt ctgttcgaga agtcacaggc
     2821 tacgtgttag tggctcttaa tcagtttcgt tacctgcctc tggagaattt acgcattatt
     2881 cgtgggacaa aactttatga ggatcgatat gccttggcaa tatttttaaa ctacagaaaa
     2941 gatggaaact ttggacttca agaacttgga ttaaagaact tgacagaaat cctaaatggt
     3001 ggagtctatg tagaccagaa caaattcctt tgttatgcag acaccattca ttggcaagat
     3061 attgttcgga acccatggcc ttccaacttg actcttgtgt caacaaatgg tagttcagga
     3121 tgtggacgtt gccataagtc ctgtactggc cgttgctggg gacccacaga aaatcattgc
     3181 cagactttga caaggacggt gtgtgcagaa caatgtgacg gcagatgcta cggaccttac
     3241 gtcagtgact gctgccatcg agaatgtgct ggaggctgct caggacctaa ggacacagac
     3301 tgctttgcct gcatgaattt caatgacagt ggagcatgtg ttactcagtg tccccaaacc
     3361 tttgtctaca atccaaccac ctttcaactg gagcacaatt tcaatgcaaa gtacacatat
     3421 ggagcattct gtgtcaagaa atgtccacat aactttgtgg tagattccag ttcttgtgtg
     3481 cgtgcctgcc ctagttccaa gatggaagta gaagaaaatg ggattaaaat gtgtaaacct
     3541 tgcactgaca tttgcccaaa agcttgtgat ggcattggca caggatcatt gatgtcagct
     3601 cagactgtgg attccagtaa cattgacaaa ttcataaact gtaccaagat caatgggaat
     3661 ttgatctttc tagtcactgg tattcatggg gacccttaca atgcaattga agccatagac
     3721 ccagagaaac tgaacgtctt tcggacagtc agagagataa caggtttcct gaacatacag
     3781 tcatggccac caaacatgac tgacttcagt gttttttcta acctggtgac cattggtgga
     3841 agagtactct atagtggcct gtccttgctt atcctcaagc aacagggcat cacctctcta
     3901 cagttccagt ccctgaagga aatcagcgca ggaaacatct atattactga caacagcaac
     3961 ctgtgttatt atcataccat taactggaca acactcttca gcacaatcaa ccagagaata
     4021 gtaatccggg acaacagaaa agctgaaaat tgtactgctg aaggaatggt gtgcaaccat
     4081 ctgtgttcca gtgatggctg ttggggacct gggccagacc aatgtctgtc gtgtcgccgc
     4141 ttcagtagag gaaggatctg catagagtct tgtaacctct atgatggtga atttcgggag
     4201 tttgagaatg gctccatctg tgtggagtgt gacccccagt gtgagaagat ggaagatggc
     4261 ctcctcacat gccatggacc gggtcctgac aactgtacaa agtgctctca ttttaaagat
     4321 ggcccaaact gtgtggaaaa atgtccagat ggcttacagg gggcaaacag tttcattttc
     4381 aagtatgctg atccagatcg ggagtgccac ccatgccatc caaactgcac ccaagggtgt
     4441 aacggtccca ctagtcatga ctgcatttac tacccatgga cgggccattc cactttacca
     4501 caacatgcta gaactcccct gattgcagct ggagtaattg gtgggctctt cattctggtc
     4561 attgtgggtc tgacatttgc tgtttatgtt agaaggaaga gcatcaaaaa gaaaagagcc
     4621 ttgagaagat tcttggaaac agagttggtg gaaccattaa ctcccagtgg cacagcaccc
     4681 aatcaagctc aacttcgtat tttgaaagaa actgagctga agagggtaaa agtccttggc
     4741 tcaggtgctt ttggaacggt ttataaaggt atttgggtac ctgaaggaga aactgtgaag
     4801 attcctgtgg ctattaagat tcttaatgag acaactggtc ccaaggcaaa tgtggagttc
     4861 atggatgaag ctctgatcat ggcaagtatg gatcatccac acctagtccg gttgctgggt
     4921 gtgtgtctga gcccaaccat ccagctggtt actcaactta tgccccatgg ctgcctgttg
     4981 gagtatgtcc acgagcacaa ggataacatt ggatcacaac tgctgcttaa ctggtgtgtc
     5041 cagatagcta agggaatgat gtacctggaa gaaagacgac tcgttcatcg ggatttggca
     5101 gcccgtaatg tcttagtgaa atctccaaac catgtgaaaa tcacagattt tgggctagcc
     5161 agactcttgg aaggagatga aaaagagtac aatgctgatg gaggaaagat gccaattaaa
     5221 tggatggctc tggagtgtat acattacagg aaattcaccc atcagagtga cgtttggagc
     5281 tatggagtta ctatatggga actgatgacc tttggaggaa aaccctatga tggaattcca
     5341 acgcgagaaa tccctgattt attagagaaa ggagaacgtt tgcctcagcc tcccatctgc
     5401 actattgacg tttacatggt catggtcaaa tgttggatga ttgatgctga cagtagacct
     5461 aaatttaagg aactggctgc tgagttttca aggatggctc gagaccctca aagataccta
     5521 gttattcagg gtgatgatcg tatgaagctt cccagtccaa atgacagcaa gttctttcag
     5581 aatctcttgg atgaagagga tttggaagat atgatggatg ctgaggagta cttggtccct
     5641 caggctttca acatcccacc tcccatctat acttccagag caagaattga ctcgaatagg
     5701 agtgaaattg gacacagccc tcctcctgcc tacaccccca tgtcaggaaa ccagtttgta
     5761 taccgagatg gaggttttgc tgctgaacaa ggagtgtctg tgccctacag agccccaact
     5821 agcacaattc cagaagctcc tgtggcacag ggtgctactg ctgagatttt tgatgactcc
     5881 tgctgtaatg gcaccctacg caagccagtg gcaccccatg tccaagagga cagtagcacc
     5941 cagaggtaca gtgctgaccc caccgtgttt gccccagaac ggagcccacg aggagagctg
     6001 gatgaggaag gttacatgac tcctatgcga gacaaaccca aacaagaata cctgaatcca
     6061 gtggaggaga acccttttgt ttctcggaga aaaaatggag accttcaagc attggataat
     6121 cccgaatatc acaatgcatc caatggtcca cccaaggccg aggatgagta tgtgaatgag
     6181 ccactgtacc tcaacacctt tgccaacacc ttgggaaaag ctgagtacct gaagaacaac
     6241 atactgtcaa tgccagagaa ggccaagaaa gcgtttgaca accctgacta ctggaaccac
     6301 agcctgccac ctcggagcac ccttcagcac ccagactacc tgcaggagta cagcacaaaa
     6361 tatttttata aacagaatgg gcggatccgg cctattgtgg cagagaatcc tgaatacctc
     6421 tctgagttct ccctgaagcc aggcactgtg ctgccgcctc caccttacag acaccggaat
     6481 actgtggtgt aactcgagtc tagatgtaca aagtggtgat aatcgaattc cgataatcaa
     6541 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6601 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6661 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6721 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6781 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6841 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6901 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     6961 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7021 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7081 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcggga attcccgcgg
     7141 ttcgaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     7201 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     7261 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     7321 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     7381 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     7441 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     7501 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     7561 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     7621 ctgttctcct cttcctcatc tccgggcctt tcgacctcac gtggccacca tgaccgagta
     7681 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     7741 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     7801 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     7861 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     7921 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     7981 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     8041 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     8101 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     8161 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     8221 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag gtacctttaa
     8281 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8341 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     8401 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8461 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8521 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     8581 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8641 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8701 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8761 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     8821 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8881 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8941 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     9001 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     9061 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     9121 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     9181 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     9241 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     9301 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     9361 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     9421 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9481 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9541 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     9601 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     9661 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     9721 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     9781 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     9841 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     9901 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     9961 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
    10021 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    10081 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    10141 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    10201 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    10261 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    10321 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    10381 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    10441 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    10501 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    10561 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    10621 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    10681 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    10741 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    10801 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    10861 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    10921 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    10981 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    11041 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    11101 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    11161 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    11221 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    11281 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    11341 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    11401 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    11461 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    11521 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    11581 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    11641 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    11701 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    11761 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
//
