LOCUS       V028565                11757 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028565
VERSION     V028565
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        3..229
                     /note="Rous sarcoma virus enhancer/promoter"
                     /label="RSV promoter"
     LTR             230..410
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    457..582
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1075..1308
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1803..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    1938..1958
                     /bound_moiety="BP Clonase™"
                     /gene="mutant version of attB"
                     /note="core recombination site for the Gateway® BP
                     reaction"
                     /label="attB4"
     enhancer        2019..2322
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2323..2526
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2566..6426
                     /label="CDON(NM_001243597)"
                     /note="CDON(NM_001243597)"
                     /gene="CDON"
     misc_feature    6472..7060
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     promoter        7091..7590
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             7607..8206
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     LTR             8278..8511
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8583..8704
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8744..8879
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        complement(8900..8918)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(8928..8944)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     rep_origin      9086..9541
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9567..9671
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             9672..10532
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      10703..11291
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    11579..11600
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        11615..11645
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11653..11669
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     11677..11693
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        11714..11732
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgta gttattaata gtaatcaatt
     1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2521 agagctggtt tagtgaaccg tcagatccaa gttggatccg ccaccatgca tccggatctt
     2581 ggacccttat gtacactgct gtatgttact cttacaattc tgtgctcttc tgtgagttca
     2641 gacttggcac cttattttac ttctgagccg ctctctgctg tccagaaact tggtggacct
     2701 gtagtactgc attgttctgc tcaacctgtg accactcgta tctcatggct gcataacgga
     2761 aaaacattgg atggaaacct ggaacatgtt aagattcatc aggggactct gacaattctt
     2821 tctctcaact cctctctttt gggttactac cagtgccttg ccaacaatag catcggtgcc
     2881 attgtgagtg gccctgcgac agtatctgtg gcagttcttg gtgattttgg ttcatccaca
     2941 aagcatgtta ttacagcaga agaaaaaagt gctggtttca ttggctgcag ggtaccggag
     3001 agtaacccca aagctgaggt gcgctataaa atccggggaa aatggctgga acattccaca
     3061 gagaattact taatccttcc atcaggaaat cttcagattt tgaatgtatc cttagaggac
     3121 aagggatcat acaaatgtgc agcttataat cctgtcacac atcaattaaa agttgaacct
     3181 attggccgaa agctccttgt gagtcgtcct tcttcagatg atgttcacat tcttcacccc
     3241 acccattcac aggcattagc tgttctttct cgtagccctg taaccttgga gtgtgtggtg
     3301 agtggggtcc cggctcctca agtgtattgg ctaaaggacg ggcaggacat tgcaccagga
     3361 agcaactgga gaaggttgta ttctcatctt gccactgata gcgttgaccc ggcggactcc
     3421 ggaaactatt cctgcatggc gggaaacaag tctggagatg taaaatatgt gacttacatg
     3481 gttaatgtac ttgaacatgc ttccatttct aaaggactac aggatcagat agtgtctctg
     3541 ggtgccacag tacactttac ctgcgacgtt catgggaacc cagcccccaa ctgtacctgg
     3601 tttcacaatg cacagcctat tcatccttct gcacgacatc taactgcagg aaacggactg
     3661 aaaatcagtg gggttactgt ggaagatgtt gggatgtatc agtgtgtagc agataatggg
     3721 attggattta tgcactctac tggaagactt gaaattgaaa atgacggtgg attcaagcca
     3781 gttataatta cggcaccagt aagtgcaaag gttgcagacg gagactttgt tactctgtcc
     3841 tgcaatgcca gtgggctgcc ggttccggtc attcgttggt atgacagcca tggattgata
     3901 accagccatc catctcaagt cctgagatcg aaatcccgaa aatcacagtt atcaagacct
     3961 gagggcttga acctggagcc tgtgtacttc gtcctgtccc aagctggtgc aagctctctc
     4021 catattcagg ctgtgactca ggaacatgcg gggaaataca tctgcgaagc tgcaaatgaa
     4081 catggtacca cacaggcaga agcatctctc atggttgttc cttttgaaac aaatacaaaa
     4141 gcagagacag tcacacttcc tgatgctgct cagaatgatg acagaagtaa gagagatggt
     4201 tcagaaactg ggttactgag ctcatttccg gtgaaggtcc atcccagtgc agtggaatca
     4261 gcaccagaga aaaacgccag cggcatctct gttcctgatg cccccatcat actgagcccc
     4321 ccacagaccc acacaccaga cacgtacaac ctggtgtgga gggcaggcaa ggatggtggg
     4381 ctgcccatca atgcttactt tgtgaagtat cgaaagctgg atgatggggt tggcatgctg
     4441 ggaagctggc acacggttcg agtcccagga agtgaaaatg agctccattt agctgagctg
     4501 gagccatcta gtctttatga agtcttgatg gtagcaagaa gcgcagcagg tgaaggccaa
     4561 cctgccatgc ttaccttccg aaccagcaaa gaaaaaacag cgtcatcaaa aaacacccag
     4621 gcatcctctc cacccgtggg catccctaag tatcccgttg tttcagaggc tgcaaacaac
     4681 aattttggag tggtacttac agattcctct aggcacagtg gagttccaga ggcaccagat
     4741 cggcctacca tctccactgc atcagagaca tcagtctatg tcacttggat tcctcgggca
     4801 aacgggggtt ctccaatcac tgccttcaaa gtcgaatata aacggatgag gaccagcaat
     4861 tggctggtgg cagctgaaga catccctcct tccaaacttt cagtggaagt tcgtagttta
     4921 gaaccaggtt caacatacaa atttagggtc attgccatca accattatgg tgagagtttt
     4981 cggagttcag catctcgtcc ttatcaagtg gttgggttcc ccaatcgctt ttccagccgt
     5041 ccaataactg gacctcacat tgcatacaca gaggctgtca gcgatactca gatcatgcta
     5101 aagtggacgt acattccatc aagtaacaat aacactccca ttcaaggatt ttatatctat
     5161 taccgaccaa cagatagtga caatgacagt gattacaaga gggatgttgt agaaggttca
     5221 aagcagtggc acatgattgg ccacctgcag ccagaaacct cctatgacat taaaatgcaa
     5281 tgcttcaatg aaggaggaga aagtgaattt agcaatgtga tgatctgcga gactaaagtg
     5341 aaacgtgttc ctggagcttc tgaatatcct gtcaaagact tgagtacccc tccaaattct
     5401 ttgggaagtg gaggaaatgt ggggcctgca accagccctg ccagaagcag tgacatgtta
     5461 tatctgatcg ttggctgtgt gctgggcgtc atggtcctca ttctgatggt tttcattgca
     5521 atgtgcctgt ggaagaatcg ccagcagaat accatacaaa aatatgaccc accaggatat
     5581 ctctaccaag gatcagatat gaacgggcag atggtggact acaccactct ctcaggagca
     5641 agtcagataa atggaaatgt tcacggaggc ttcctaacca atggcggtct cagcagtggc
     5701 tattcccacc ttcaccataa ggtccccaat gcagtcaatg gaattgtgaa tgggagccta
     5761 aatggagggc tttactccgg gcacagcaac tctctaacca ggacacacgt ggattttgaa
     5821 catcctcatc atctagtgaa tggtggtgga atgtacacgg ccgtgcctca gattgaccct
     5881 ctggagtgtg ttaactgccg aaattgtcga aacaacaata ggtgtttcac caaaaccaac
     5941 agcactttca gcagcagccc tcctcctgtg gtccctgtgg tagcacctta tcctcaggat
     6001 ggtttggaaa tgaagcccct cagtcacgtg aaggtgcctg tatgcctgac ttccgcagtc
     6061 cctgattgtg gccagttgcc ggaggagagc gtcaaggaca atgtggaacc agtccctact
     6121 cagcgtacct gctgtcagga cattgtaaat gacgtcagct ctgatggctc agaagatcca
     6181 gcagagttca gcagaggtca ggaaggcatg atcaacctta ggattccaga tcatcttcag
     6241 ttagctaaga gctgtgtatg ggaaggagac agctgtgccc attcagaaac agagatcaac
     6301 attgtaagtt ggaatgctct tattttgcca cctgtccccg agggctgtgc tgagaagaca
     6361 atgtggtctc cacctggcat tcctttagac agcccgacag aggtccttca gcagccccgg
     6421 gaaacctgac tcgagtctag atgtacaaag tggtgataat cgaattccga taatcaacct
     6481 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6541 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6601 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6661 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6721 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6781 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6841 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     6901 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6961 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7021 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgggaatt cccgcggttc
     7081 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     7141 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     7201 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     7261 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     7321 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     7381 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     7441 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     7501 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     7561 ttctcctctt cctcatctcc gggcctttcg acctcacgtg gccaccatga ccgagtacaa
     7621 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     7681 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     7741 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     7801 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     7861 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     7921 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     7981 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     8041 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     8101 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     8161 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaggta cctttaagac
     8221 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     8281 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     8341 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8401 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8461 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     8521 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     8581 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8641 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8701 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     8761 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8821 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8881 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     8941 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     9001 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     9061 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     9121 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     9181 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     9241 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     9301 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     9361 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     9421 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     9481 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     9541 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     9601 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     9661 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     9721 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     9781 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     9841 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     9901 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     9961 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    10021 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    10081 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    10141 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    10201 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    10261 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    10321 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    10381 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    10441 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10501 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    10561 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    10621 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    10681 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    10741 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10801 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    10861 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10921 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    10981 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    11041 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    11101 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    11161 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11221 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11281 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    11341 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    11401 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    11461 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    11521 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    11581 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    11641 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    11701 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagctt
//
