LOCUS       62056_13310        6573 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     424..440
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(509..525)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(533..549)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(557..587)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(602..623)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(911..1499)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1703..2503
                     /codon_start=1
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     CDS             complement(3446..4885)
                     /codon_start=1
                     /label=pSG5 Rep
                     /note="replication initiator protein from the Streptomyces 
                     ghanaensis plasmid pSG5"
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
                     AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
                     IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
                     SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
                     GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
                     RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
                     PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
                     ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
                     APVLQPWGQHPGDCLRVDTAVA"
     oriT            6052..6161
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 gatctacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
       61 agctctcgca gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
      121 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt gatcggcact
      181 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattt atgcggtgtg
      241 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
      301 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      361 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      421 gttgtaaaac gacggccagt gccaagcttg ggctgcaggt cgactctaga ggatccgcgg
      481 ccgcgcgcga tatcgaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc
      541 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      601 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      661 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      721 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      781 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      841 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      901 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      961 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1021 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1081 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1141 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1201 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1261 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1321 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1381 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1441 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1501 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1561 ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg gttcatgtgc
     1621 agctccatca gcaaaagggg atgataagtt tatcaccacc gactatttgc aacagtgccg
     1681 ttgatcgtgc tatgatcgac tgatgtcatc agcggtggag tgcaatgtcg tgcaatacga
     1741 atggcgaaaa gccgagctca tcggtcagct tctcaacctt ggggttaccc ccggcggtgt
     1801 gctgctggtc cacagctcct tccgtagcgt ccggcccctc gaagatgggc cacttggact
     1861 gatcgaggcc ctgcgtgctg cgctgggtcc gggagggacg ctcgtcatgc cctcgtggtc
     1921 aggtctggac gacgagccgt tcgatcctgc cacgtcgccc gttacaccgg accttggagt
     1981 tgtctctgac acattctggc gcctgccaaa tgtaaagcgc agcgcccatc catttgcctt
     2041 tgcggcagcg gggccacagg cagagcagat catctctgat ccattgcccc tgccacctca
     2101 ctcgcctgca agcccggtcg cccgtgtcca tgaactcgat gggcaggtac ttctcctcgg
     2161 cgtgggacac gatgccaaca cgacgctgca tcttgccgag ttgatggcaa aggttcccta
     2221 tggggtgccg agacactgca ccattcttca ggatggcaag ttggtacgcg tcgattatct
     2281 cgagaatgac cactgctgtg agcgctttgc cttggcggac aggtggctca aggagaagag
     2341 ccttcagaag gaaggtccag tcggtcatgc ctttgctcgg ttgatccgct cccgcgacat
     2401 tgtggcgaca gccctgggtc aactgggccg agatccgttg atcttcctgc atccgccaga
     2461 ggcgggatgc gaagaatgcg atgccgctcg ccagtcgatt ggctgagctc atgagcggag
     2521 aacgagatga cgttggaggg gcaaggtcgc gctgattgct ggggcaacac gtggagcgga
     2581 tcggggattg tctttcttca gctcgctgat gatatgctga cgctcaatgc cgtttggcct
     2641 ccgactaacg aaaatcccgc atttggacgg ctgatccgat tggcacggcg gacggcgaat
     2701 ggcggagcag acgctcgtcc gggggcaatg agatatgaaa aagcctgaac tcaccgcgac
     2761 gtatcgatgt cgaggttcct caggggagcc accccagaga agccctcgga gctgagcgga
     2821 gctatttcca aagccatgcc agctagagac agtgcacacc gccaagcatc tgcaaaaccc
     2881 tcgcctggag ggaaagtgca atgtacgtac gctggtttcc ctccagaaag ggattctgcg
     2941 gcttctcaac tttggaagaa gaggcggaac gaactgctgt ttcagcctac tcatgtgagg
     3001 aggctggtcc tttaccctga tgcccgaggg catcagggta aaggaccagc ctcgccaact
     3061 aggagcgcta ccaaggcgaa cacaacccag gacttggtag aacccttcgc cggagaagtt
     3121 cagactcatc ggcatgagca cccctcgccg cgcactcggc accggtccgt cgaccaccac
     3181 caggacgtcg ttgtcgacgt cggccccgcg gctcctgccc gccgaacgcg tcgtcgtcga
     3241 cggcctggtg ctcatcgacg agcacccgga gccaggtgaa aagcgccggc ggacgctcgg
     3301 actgggcgcg ggattccagc agtaacccag gtccgccggc cacctcacgg caggcagacc
     3361 ctcggctttc gcgccgggac cgccatgaga ccgccacccg gatgtccggg gtggcggtct
     3421 catgggcggt ccctcagcgg ccctacgcga ccgctgtgtc gacgcggagg cagtctccgg
     3481 ggtgctggcc ccagggctgg agtaccgggg cgagcttgcc cttgcagcgg cggcacacgg
     3541 gcgacgcggc gccgggcggg ggagtcttga cgtcgacggt gaccggcttc ggcttcaggc
     3601 gcttccgaag gctgatcgtc gggcggatcg cctccttctt cggctgacgc actcggtcga
     3661 gcgaagcggc cagctcctcc gtgcgggcct cgttggcctt ccggcgcgcc tcgcggaaag
     3721 cagcttcctc ctcggacatg acctcgaacc tcatctggtc agcgtcaagg tcgcccggcg
     3781 cggccggcgc ttccggggcg ggcgggtcca ggacgtcctt gccccacacc aggccccagg
     3841 actcgacgag ccgccggacg cccggtaggc cgtacgtctc ggcgaccttg atgaggtcga
     3901 ggcgacgtcc ggcgacgcgg gcgatgtatc ggtaccagat gtaggccggg atgaccgcga
     3961 tggcgaccag gccctcggtg tcgtcggtga tctcctcctc ggtgcggacg tcctgctgga
     4021 tgccgagttc cttgatcagc cggttcaggt tctgcgaccg gtagtgcttg cggacctgga
     4081 agacgccgaa ctcgcgctcg cggtacttct cgacgaacgg gccgggccga cgaagccgct
     4141 gcagctcggc ggccgccgcg tcgcccaggt cgagcggtcc catgcggtcg tcgccgcgac
     4201 cggccttgaa gttctgtccg gccagctcca ggccgatctt ggcgacgccg cccttggtct
     4261 tgtcgccgtc cttgtagagg tagcgggcct gcttgcccgc atcgccgtca gcggcgtccg
     4321 cgccgttgag tgggcgcacg tcggtgccgt ggcccttgcc ctcacaggag caaccgggcc
     4381 ggtcgcacgt ctcgctgacg gtgtagccgc ccgcggattc gaccccggcg gcccaggctc
     4441 cggcgagtgc gtcgcggaac gcggcctggg cgtccgggcc gagcacctcg cgggtgaccc
     4501 agagcgtgtg ccagtgcagg tgccagccgg agccccagcc gaaggtgtcc tcgaaggccc
     4561 gctcgtagcc gatgatcccg aagtcgtcgc gcatcgtgcg ccagcggcgg ccggacgagc
     4621 cgtacgcgcc cttccagccg tcgtgcaaga ccgcgaccag gccgtgccgc attcccttgc
     4681 ggacggtgcc gaacgccatg cgctcgaagt ggcgcaacgt gttcgtgcca aggtgcagcc
     4741 cgtacccggc gtccgcgaga ccgtcggcgg cgagctgcac gttcgagccc cgtacggcca
     4801 ggatgcggct catgcaccac gggcaggtgt ggacgttgtt gcagcggcac gtgttgcccc
     4861 acgtcgcctc gcccggcttc cacatcagct cggccgtccc ggcagtgagc cgggtcccgc
     4921 agcccttgaa cgcctcgttc agcgacaccg tctggtgccg gtcccgccgg gcgaaccgct
     4981 cgtcgcgcgg gtcctcccgc ccggctgtcg cggcaccctc gtttggggta gaacccgttc
     5041 cagttacagc gctctgacct gcagtggacg gagattttcc cttactacta aagcccgcgt
     5101 ccggattacc cgctgtagtc gtgcttgcta cgctgcgtga ctggtccgca atgagacgct
     5161 ttgcgcgctt tcggcaggcg tccgagcagt agattttggg gcgcttcccg gggatgtgga
     5221 cgatcggggt gccgcagtgg cacttcggtc cggcggggcg cggtggtgtc gacgcgctgt
     5281 tctctcgtac gctcgtcaca gagcaaacgt cctcactcgg catgctgcgc cggttcgggg
     5341 gcggcgagcc cgggaggcca atcccgggct cgtgccattt ctgggtcctg ttgatcctgg
     5401 cattggtgtg gccgttcatt gcccctgctc gctcctgacg cgccgataga cgtccgatac
     5461 gcccggtgct ggtgggattt gataggtcgg aagaagcccc gcccgggcct gggcggggct
     5521 tcctgtgcgt caggacctcc tcgtcgtgag cctcttcggc ctatggacgg agtgacctcg
     5581 tgatccgtta cagccgcgcg cgctcgcgta gagcggtctc atcagttcca cgaacggtcc
     5641 tcttcgcaga tcagggcgtt ggggcggagt ctcaccaagg actacgtctg ctggcgattt
     5701 ccgttacacc ccgggcggtg gccggcgcac acgcgcgccc gcgttgggca gtgcagaaag
     5761 tgcagaaacc taggcgctga tggtccaggt ccacggttcg tcgtcggcgg cggcgcgggc
     5821 ggcggcgtcg gccagggcgc gggcgagacc ggctacggcg ggcttgatgc gccggttgcg
     5881 ggcgaccttg agcagctagt atgcaggtcg acggatcttt tccgctgcat aaccctgctt
     5941 cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat atacaggatt
     6001 ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc cgggcaggat
     6061 aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt attcgcacct
     6121 ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gcatgagcct gtcggcctac
     6181 ctgctggccg tcggccaggg ctacaaaatc acgggcgtcg tggactatga gcacgtccgc
     6241 gagctggccc gcatcaatgg cgacctgggc cgcctgggcg gcctgctgaa actctggctc
     6301 accgacgacc cgcgcacggc gcggttcggt gatgccacga tcctcgccct gctggcgaag
     6361 atcgaagaga agcaggacga gcttggcaag gtcatgatgg gcgtggtccg cccgagggca
     6421 gagccatgac ttttttagcc gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt
     6481 ccccatgcgc tccatcaaga agagcgactt cgcggagctg gtgaagtaca tcaccgacga
     6541 gcaaggcaag accgatcccc ggggacctgc ata
//
