LOCUS V036856 5880 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V036856 VERSION V036856 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 625..1131 /label="EREG(NM_001432)" /note="EREG(NM_001432)" /gene="EREG" CDS 1132..1197 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 1239..1825 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES2" CDS 1826..2545 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 2668..2789 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2796..3251) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3278..3382 /gene="bla" /label="AmpR promoter" promoter 3384..3741 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3776..4570 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4802..4849 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5178..5766 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgaggc caccatgacc gcggggagga ggatggagat gctctgtgcc 661 ggcagggtcc ctgcgctgct gctctgcctg ggtttccatc ttctacaggc agtcctcagt 721 acaactgtga ttccatcatg tatcccagga gagtccagtg ataactgcac agctttagtt 781 cagacagaag acaatccacg tgtggctcaa gtgtcaataa caaagtgtag ctctgacatg 841 aatggctatt gtttgcatgg acagtgcatc tatctggtgg acatgagtca aaactactgc 901 aggtgtgaag tgggttatac tggtgtccga tgtgaacact tctttttaac cgtccaccaa 961 cctttaagca aagaatatgt ggctttgacc gtgattctta ttattttgtt tcttatcaca 1021 gtcgtcggtt ccacatatta tttctgcaga tggtacagaa atcgaaaaag taaagaacca 1081 aagaaggaat atgagagagt tacctcaggg gatccagagt tgccgcaagt cgactacaaa 1141 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtag 1201 gaattctgca gtcgacggta ccgcgggccc gggatccgcc cctctccctc ccccccccct 1261 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt 1321 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg 1381 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc 1441 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt 1501 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta 1561 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg 1621 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag 1681 gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac atgtgtttag 1741 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa 1801 acacgatgat aatatggcca caaccatggt gagcaagggc gaggagctgt tcaccggggt 1861 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 1921 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 1981 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt 2041 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 2101 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 2161 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 2221 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 2281 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat 2341 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 2401 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc 2461 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 2521 cggcatggac gagctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc 2581 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa 2641 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa 2701 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 2761 ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt 2821 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg 2881 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg 2941 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta 3001 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg 3061 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa 3121 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct 3181 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct 3241 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 3301 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 3361 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag 3421 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 3481 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 3541 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc 3601 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 3661 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 3721 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat 3781 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 3841 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 3901 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga 3961 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 4021 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 4081 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 4141 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 4201 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 4261 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga 4321 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 4381 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 4441 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 4501 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 4561 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca 4621 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc 4681 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac 4741 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg 4801 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg 4861 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac 4921 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc 4981 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag 5041 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 5101 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 5161 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 5221 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 5281 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg 5341 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 5401 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 5461 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 5521 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 5581 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 5641 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 5701 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 5761 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 5821 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat //