LOCUS       V027310                 6872 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027310
VERSION     V027310
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             624..2189
                     /label="CYP26C1(NM_183374)"
                     /note="CYP26C1(NM_183374)"
                     /gene="CYP26C1"
     misc_feature    2231..2817
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             2818..3537
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    3660..3781
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3788..4243)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4270..4374
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4376..4733
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4768..5562
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5794..5841
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6170..6758
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagcc accatgttcc cttgggggct gagctgcctg tcagtgctgg
      661 gggcggcggg cactgctctc ctgtgcgcgg gcctgctgct cagcctggcc cagcacctct
      721 ggaccctccg ctggatgctg agccgggacc gggcctccac cctgcctctg cccaagggct
      781 ccatggggtg gcccttcttc ggcgaaacgc tgcactggtt agttcagggc tcgcgcttcc
      841 acagttctcg ccgagagcgc tatgggacag tgttcaagac gcacctgctg ggcaggccag
      901 tgatccgcgt gagcggcgcg gagaacgtgc gcaccatcct gctgggcgag caccgcctgg
      961 tgcgcagcca gtggccgcag agtgcgcaca tcctgctggg ctcgcacaca ctgctaggtg
     1021 cggtcggcga gccgcaccgg cggcggcgca aggtcctggc gcgcgtgttc agccgcgccg
     1081 cgctggagcg ctacgtgccg cgcctgcagg gggcgctgcg gcatgaggtg cgctcctggt
     1141 gcgcggcggg cgggccggtc tcagtctacg acgcctccaa agcgctcacc ttccgcatgg
     1201 ccgcgcgcat cctgctgggg ttgcggctgg acgaggcgca gtgcgccacg ctggcccgga
     1261 ccttcgagca gctcgtggag aacctcttct cactgcctct ggacgttccc ttcagtggcc
     1321 tacgcaaggg catccgggca agggaccagc tgcatcggca cctggagggg gccatttctg
     1381 agaagcttca cgaggacaag gctgcagagc cgggtgatgc cctcgaccta atcattcaca
     1441 gtgcaaggga gctgggccat gagccctcca tgcaggagct gaaggagtcg gctgtggagc
     1501 tcctcttcgc cgccttcttc accacggcca gtgccagcac ctcgctcgtc ctgctgctac
     1561 tgcagcatcc ggcggccatc gccaagattc gggaggagct ggtggcgcag gggctggggc
     1621 gcgcgtgcgg ctgcgcgccc ggggccgctg ggggcagcga ggggcccccg cccgactgcg
     1681 gctgcgagcc cgacctcagc ctcgcggcgc tgggccgtct gcgctacgtc gactgcgtgg
     1741 tcaaggaggt gctgcgcctc ctgccgccag tgtccggggg ctaccgcacc gccctgcgca
     1801 ccttcgagct cgacggctac cagatcccca agggctggag cgtgatgtat agcatccggg
     1861 acacgcacga gacggctgcg gtgtaccgca gccctcccga aggcttcgat ccagagcgct
     1921 tcggcgcagc gcgcgaagat tcccggggcg cctccagccg cttccattac atcccgttcg
     1981 gcggcggtgc gcgcagctgc ctcggccagg agctggcgca agccgtgctc cagctgctag
     2041 ctgtggagct agtgcgcacc gcgcgctggg aactggccac acccgccttc cccgccatgc
     2101 agacggtgcc catcgtgcac ccagtggacg ggctgcggct ctttttccac cccctcacgc
     2161 cttcggttgc ggggaatggg ctatgcctct gagaattctg cagtcgacgg taccgcgggc
     2221 ccgggatccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     2281 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     2341 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     2401 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     2461 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     2521 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     2581 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     2641 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     2701 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     2761 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg
     2821 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     2881 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     2941 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     3001 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     3061 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     3121 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     3181 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     3241 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     3301 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     3361 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     3421 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     3481 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc
     3541 ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     3601 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     3661 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3721 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3781 aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     3841 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     3901 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     3961 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     4021 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     4081 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     4141 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     4201 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
     4261 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4321 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag
     4381 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     4441 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     4501 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4561 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4621 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     4681 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat
     4741 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     4801 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     4861 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     4921 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
     4981 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     5041 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     5101 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     5161 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     5221 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     5281 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     5341 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     5401 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     5461 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     5521 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     5581 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     5641 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     5701 gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga
     5761 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     5821 acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     5881 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     5941 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     6001 ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6061 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6121 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6181 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6241 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6301 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6361 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6421 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6481 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6541 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6601 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6661 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6721 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6781 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6841 attctgtgga taaccgtatt accgccatgc at
//
