LOCUS       62056_12920        8337 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1103..2035
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             2342..3991
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(4041..4162)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4348..4803
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4920..5277
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5328..6119
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6186..6234
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        6540..6644
                     /label=AmpR promoter
     CDS             6645..7502
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7676..8264
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc ctcgaggcca ccatggcttc caaggtgtac gaccccgagc aacgcaaacg
     1141 catgatcact gggcctcagt ggtgggctcg ctgcaagcaa atgaacgtgc tggactcctt
     1201 catcaactac tatgattccg agaagcacgc cgagaacgcc gtgatttttc tgcatggtaa
     1261 cgctgcctcc agctacctgt ggaggcacgt cgtgcctcac atcgagcccg tggctagatg
     1321 catcatccct gatctgatcg gaatgggtaa gtccggcaag agcgggaatg gctcatatcg
     1381 cctcctggat cactacaagt acctcaccgc ttggttcgag ctgctgaacc ttccaaagaa
     1441 aatcatcttt gtgggccacg actggggggc ttgtctggcc tttcactact cctacgagca
     1501 ccaagacaag atcaaggcca tcgtccatgc tgagagtgtc gtggacgtga tcgagtcctg
     1561 ggacgagtgg cctgacatcg aggaggatat cgccctgatc aagagcgaag agggcgagaa
     1621 aatggtgctt gagaataact tcttcgtcga gaccatgctc ccaagcaaga tcatgcggaa
     1681 actggagcct gaggagttcg ctgcctacct ggagccattc aaggagaagg gcgaggttag
     1741 acggcctacc ctctcctggc ctcgcgagat ccctctcgtt aagggaggca agcccgacgt
     1801 cgtccagatt gtccgcaact acaacgccta ccttcgggcc agcgacgatc tgcctaagat
     1861 gttcatcgag tccgaccctg ggttcttttc caacgctatt gtcgagggag ctaagaagtt
     1921 ccctaacacc gagttcgtga aggtgaaggg cctccacttc agccaggagg acgctccaga
     1981 tgaaatgggt aagtacatca agagcttcgt ggagcgcgtg ctgaagaacg agcagtaagt
     2041 cgacgctagc tcgggccggg aggagccgca gccggaggag ggggaggagg aagaagagaa
     2101 ggaagaggag agggggccgc agtggcgact cggcgctcgg aagccgggct catggacggg
     2161 tgaggcggcg gtgtgcgcag acagtgctcc agccgcgcgc gctccccagg ccctggcccg
     2221 ggcctcgggc cggggaggaa gagtagctcg ccgaggcgcc gaggagagcg ggccgcccca
     2281 cagcccgagc cggagaggga gcgcgagccg cgccggcccc ggtcgggcct ccgaaaccat
     2341 gatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg
     2401 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat
     2461 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc
     2521 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt
     2581 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt
     2641 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat
     2701 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca
     2761 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg
     2821 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga
     2881 ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg
     2941 atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc
     3001 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt
     3061 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt
     3121 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta
     3181 caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct
     3241 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc
     3301 taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata
     3361 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg
     3421 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa
     3481 aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg
     3541 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc
     3601 tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc
     3661 tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca
     3721 acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact
     3781 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga
     3841 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga
     3901 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat
     3961 aaaggccaag aagggcggaa agatcgccgt gtaagcggcc gcttcccttt agtgagggtt
     4021 aatgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     4081 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     4141 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     4201 gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatccg
     4261 ataaggatcg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4321 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
     4381 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4441 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4501 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4561 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4621 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4681 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4741 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     4801 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgcgg
     4861 atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt aggtaccttc
     4921 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     4981 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5041 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5101 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5161 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5221 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     5281 cttgattctt ctgacacaac agtctcgaac ttaaggctag agccaccatg attgaacaag
     5341 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     5401 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     5461 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     5521 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     5581 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     5641 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     5701 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     5761 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     5821 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     5881 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     5941 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6001 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6061 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6121 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgatg
     6181 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga
     6241 tagcgataag gatccgcgta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     6301 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     6361 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     6421 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     6481 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     6541 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6601 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6661 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6721 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6781 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6841 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6901 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6961 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7021 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7081 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7141 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7201 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7261 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7321 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7381 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7441 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7501 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7561 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7621 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7681 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7741 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7801 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7861 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7921 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7981 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8041 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8101 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8161 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8221 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8281 cctttttacg gttcctggcc ttttgctggc cttttgctca catggctcga cagatct
//
