LOCUS       62056_12705        5923 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1891..1907
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2184..2200)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2208..2224)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2232..2262)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2277..2298)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2586..3174)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             3378..4178
                     /codon_start=1
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     CDS             complement(4615..4983)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(5016..5125)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(5363..5374)
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     protein_bind    5445..5544
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     CDS             join(5554..5923,1..1445)
                     /codon_start=1
                     /label=phage phi-C31 integrase
                     /note="integrase from phage phi-C31"
                     /translation="VDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
ORIGIN
        1 tccggcaggg aaacgtcatg gacctgattc acctgattat gcggctcgac gcgtcgcaca
       61 aagaatcttc gctgaagtcg gcgaagattc tcgacacgaa gaaccttcag cgcgaattgg
      121 gcgggtacgt cggcgggaag gcgccttacg gcttcgagct tgtttcggag acgaaggaga
      181 tcacgcgcaa cggccgaatg gtcaatgtcg tcatcaacaa gcttgcgcac tcgaccactc
      241 cccttaccgg acccttcgag ttcgagcccg acgtaatccg gtggtggtgg cgtgagatca
      301 agacgcacaa acaccttccc ttcaagccgg gcagtcaagc cgccattcac ccgggcagca
      361 tcacggggct ttgtaagcgc atggacgctg acgccgtgcc gacccggggc gagacgattg
      421 ggaagaagac cgcttcaagc gcctgggacc cggcaaccgt tatgcgaatc cttcgggacc
      481 cgcgtattgc gggcttcgcc gctgaggtga tctacaagaa gaagccggac ggcacgccga
      541 ccacgaagat tgagggttac cgcattcagc gcgacccgat cacgctccgg ccggtcgagc
      601 ttgattgcgg accgatcatc gagcccgctg agtggtatga gcttcaggcg tggttggacg
      661 gcagggggcg cggcaagggg ctttcccggg ggcaagccat tctgtccgcc atggacaagc
      721 tgtactgcga gtgtggcgcc gtcatgactt cgaagcgcgg ggaagaatcg atcaaggact
      781 cttaccgctg ccgtcgccgg aaggtggtcg acccgtccgc acctgggcag cacgaaggca
      841 cgtgcaacgt cagcatggcg gcactcgaca agttcgttgc ggaacgcatc ttcaacaaga
      901 tcaggcacgc cgaaggcgac gaagagacgt tggcgcttct gtgggaagcc gcccgacgct
      961 tcggcaagct cactgaggcg cctgagaaga gcggcgaacg ggcgaacctt gttgcggagc
     1021 gcgccgacgc cctgaacgcc cttgaagagc tgtacgaaga ccgcgcggca ggcgcgtacg
     1081 acggacccgt tggcaggaag cacttccgga agcaacaggc agcgctgacg ctccggcagc
     1141 aaggggcgga agagcggctt gccgaacttg aagccgccga agccccgaag cttccccttg
     1201 accaatggtt ccccgaagac gccgacgctg acccgaccgg ccctaagtcg tggtgggggc
     1261 gcgcgtcagt agacgacaag cgcgtgttcg tcgggctctt cgtagacaag atcgttgtca
     1321 cgaagtcgac tacgggcagg gggcagggaa cgcccatcga gaagcgcgct tcgatcacgt
     1381 gggcgaagcc gccgaccgac gacgacgaag acgacgccca ggacggcacg gaagacgtag
     1441 cggcgtagcg agacacccgg gaagcctgat ctacgtctgt cgagaagttt ctgatcgaaa
     1501 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     1561 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     1621 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     1681 ttgacattgg ggaatttatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     1741 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1801 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1861 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttgggc
     1921 tgcaggtcga ctctagtatg catgcgagtg tccgttcgag tggcggcttg cgcccgatgc
     1981 tagtcgcggt tgatcggcga tcgcaggtgc acgcggtcga tcttgacggc tggcgagagg
     2041 tgcggggagg atctgaccga cgcggtccac acgtggcacc gcgatgctgt tgtgggcaca
     2101 atcgtgccgg ttggtaggat ccacatatgt tggggatcct ctagaggatc cgcggccgcg
     2161 cgcgatatcg aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc
     2221 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     2281 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     2341 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2401 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2461 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2521 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2581 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2641 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2701 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2761 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2821 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2881 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2941 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3001 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3061 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3121 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3181 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3241 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttggttca tgtgcagctc
     3301 catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag tgccgttgat
     3361 cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa tgtcgtgcaa tacgaatggc
     3421 gaaaagccga gctcatcggt cagcttctca accttggggt tacccccggc ggtgtgctgc
     3481 tggtccacag ctccttccgt agcgtccggc ccctcgaaga tgggccactt ggactgatcg
     3541 aggccctgcg tgctgcgctg ggtccgggag ggacgctcgt catgccctcg tggtcaggtc
     3601 tggacgacga gccgttcgat cctgccacgt cgcccgttac accggacctt ggagttgtct
     3661 ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg
     3721 cagcggggcc acaggcagag cagatcatct ctgatccatt gcccctgcca cctcactcgc
     3781 ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg
     3841 gacacgatgc caacacgacg ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg
     3901 tgccgagaca ctgcaccatt cttcaggatg gcaagttggt acgcgtcgat tatctcgaga
     3961 atgaccactg ctgtgagcgc tttgccttgg cggacaggtg gctcaaggag aagagccttc
     4021 agaaggaagg tccagtcggt catgcctttg ctcggttgat ccgctcccgc gacattgtgg
     4081 cgacagccct gggtcaactg ggccgagatc cgttgatctt cctgcatccg ccagaggcgg
     4141 gatgcgaaga atgcgatgcc gctcgccagt cgattggctg agctcatgag cggagaacga
     4201 gatgacgttg gaggggcaag gtcgcgctga ttgctggggc aacacgtgga gcggatcggg
     4261 gattgtcttt cttcagctcg ctgatgatat gctgacgctc aatgccgttt ggcctccgac
     4321 taacgaaaat cccgcatttg gacggctgat ccgattggca cggcggacgg cgaatggcgg
     4381 agcagacgct cgtccggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtatc
     4441 gggccctggc cagctagcta gagtcgacct gcaggtcccc ggggatcggt cttgccttgc
     4501 tcgtcggtga tgtacttcac cagctccgcg aagtcgctct tcttgatgga gcgcatgggg
     4561 acgtgcttgg caatcacgcg caccccccgg ccgttttagc ggctaaaaaa gtcatggctc
     4621 tgccctcggg cggaccacgc ccatcatgac cttgccaagc tcgtcctgct tctcttcgat
     4681 cttcgccagc agggcgagga tcgtggcatc accgaaccgc gccgtgcgcg ggtcgtcggt
     4741 gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg ccattgatgc gggccagctc
     4801 gcggacgtgc tcatagtcca cgacgcccgt gattttgtag ccctggccga cggccagcag
     4861 gtaggccgac aggctcatgc cggccgccgc cgccttttcc tcaatcgctc ttcgttcgtc
     4921 tggaaggcag tacaccttga taggtgggct gcccttcctg gttggcttgg tttcatcagc
     4981 catccgcttg ccctcatctg ttacgccggc ggtagccggc cagcctcgca gagcaggatt
     5041 cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg
     5101 tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac
     5161 acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat
     5221 cgctataatg accccgaagc agggttatgc agcggaaaag atccgtcgac ctgcaggcat
     5281 gcaagctcta gcgattccag acgtcccgaa ggcgtggcgc ggcttccccg tgccggagca
     5341 atcgccctgg gtgggttaca cgacgcccct ctatggcccg tactgacgga cacaccgaag
     5401 ccccggcggc aaccctcagc ggatgccccg gggcttcacg ttttcccagg tcagaagcgg
     5461 ttttcgggag tagtgcccca actggggtaa cctttgagtt ctctcagttg ggggcgtagg
     5521 gtcgccgaca tgacacaagg ggttgtgacc ggggtggaca cgtacgcggg tgcttacgac
     5581 cgtcagtcgc gcgagcgcga aaattcgagc gcagcaagcc cagcgacaca gcgtagcgcc
     5641 aacgaagaca aggcggccga ccttcagcgc gaagtcgagc gcgacggggg ccggttcagg
     5701 ttcgtcgggc atttcagcga agcgccgggc acgtcggcgt tcgggacggc ggagcgcccg
     5761 gagttcgaac gcatcctgaa cgaatgccgc gccgggcggc tcaacatgat cattgtctat
     5821 gacgtgtcgc gcttctcgcg cctgaaggtc atggacgcga ttccgattgt ctcggaattg
     5881 ctcgccctgg gcgtgacgat tgtttccact caggaaggcg tct
//
