LOCUS       Exported                2980 bp DNA     circular SYN 23-JAN-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2980)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(335..2980,1..334)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        80..283
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        328..346
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      377..410
                     /label=pol I terminator
                     /note="murine terminator"
     promoter        complement(430..641)
                     /label=pol I promoter
                     /note="human pol I promoter
                     "
     polyA_signal    689..913
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1172..1760)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1931..2791)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2792..2896)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
       61 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      121 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      181 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      241 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
      301 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgt
      361 taacgctagc agttaaccgg agtactggtc gacctccgaa gttggggggg aggagacggt
      421 accgtctcca ataacccggc ggcccaaaat gccgactcgg agcgaaagat atacctcccc
      481 cggggccggg aggtcgcgtc accgaccacg ccgccggccc aggcgacgcg cgacacggac
      541 acctgtcccc aaaaacgcca ccatcgcagc cacacacgga gcgcccgggg ccctctggtc
      601 aaccccagga cacacgcggg agcagcgccg ggccggggac gccctcccgg cggtcaccta
      661 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
      721 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
      781 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
      841 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
      901 gtgggctcta tggcttctga ggcggaaaga accagctgca ttaatgaatc ggccaacgcg
      961 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1021 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1081 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1141 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1201 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1261 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1321 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1381 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1441 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1501 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1561 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1621 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1681 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1741 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1801 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1861 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1921 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1981 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2041 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2101 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2161 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2221 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2281 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2341 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2401 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2461 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2521 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2581 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     2641 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     2701 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     2761 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     2821 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     2881 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgat atgccaagta
     2941 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
//
