LOCUS       V036259                11569 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036259
VERSION     V036259
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2058..2175
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     enhancer        2227..2530
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2531..2734
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2815..2824
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2824..2895
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags"
                     /transl_table=1
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
                     /label="3xFLAG"
     CDS             2929..6786
                     /label="NEK1(NM_001199397)"
                     /note="NEK1(NM_001199397)"
                     /gene="NEK1"
     promoter        6826..7325
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             7346..7945
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7959..8547
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8622..8855
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        9060..9164
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             9165..10025
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      10196..10784
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    11195..11216
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        11231..11261
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11269..11285
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     11293..11309
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccac gcgtgccacc
     2821 atggattaca aggatgacga cgataaggac tataaggacg atgatgacaa ggactacaaa
     2881 gatgatgacg ataaacgaag cccgggcgga tcgggtttaa acggatccat ggagaagtat
     2941 gttagactac agaagattgg agaaggttca tttggaaaag ccattcttgt taaatctaca
     3001 gaagatggca gacagtatgt tatcaaggaa attaacatct caagaatgtc cagtaaagaa
     3061 agagaagaat caaggagaga agttgcagta ttggcaaaca tgaagcatcc aaatattgtc
     3121 cagtatagag aatcatttga agaaaatggc tctctctaca tagtaatgga ttactgtgag
     3181 ggaggggatc tgtttaagcg aataaatgct cagaaaggcg ttttgtttca agaggatcag
     3241 attttggact ggtttgtaca gatatgtttg gccctgaaac atgtacatga tagaaaaatt
     3301 cttcatcgag acattaaatc tcagaacata tttttaacta aagatggaac agtacaactt
     3361 ggagattttg gaattgctag agttcttaat agtactgtag agctggctcg aacttgcata
     3421 gggaccccat actacttgtc acctgaaatc tgtgaaaaca aaccttacaa taataaaagt
     3481 gacatttggg ctctggggtg tgtcctttat gagctgtgta cacttaaaca tgcttttgaa
     3541 gctggcagta tgaaaaacct ggtactgaag ataatatctg gatcttttcc acctgtgtct
     3601 ttgcattatt cctatgatct ccgcagtttg gtgtctcagt tatttaaaag aaatcctagg
     3661 gatagaccat cagtcaactc catattggag aaaggtttta tagccaaacg cattgaaaag
     3721 tttctctctc ctcagcttat tgcagaagaa ttttgtctaa aaacattttc gaagtttgga
     3781 tcacagccta taccagctaa aagaccagct tcaggacaaa actcgatttc tgttatgcct
     3841 gctcagaaaa ttacaaagcc tgccgctaaa tatggaatac ctttagcata taagaaatat
     3901 ggagataaaa aattacacga aaagaaacca ctgcaaaaac ataaacaggc ccatcaaact
     3961 ccagagaaga gagtgaatac tggagaagaa aggaggaaaa tatctgagga agcagcaaga
     4021 aagagaaggc tggaatttat tgaaaaagaa aagaaacaaa aggatcagat tattagttta
     4081 atgaaggctg aacaaatgaa aaggcaagaa aaggaaaggt tggaaagaat aaatagggcc
     4141 agggaacaag gatggagaaa tgtgctaagt gctggtggaa gtggtgaagt aaaggctcct
     4201 tttctgggca gtggagggac tatagctcca tcatcttttt cttctcgagg acagtatgaa
     4261 cattaccatg ccatttttga ccaaatgcag caacaaagag cagaagataa tgaagctaaa
     4321 tggaaaagag aaatatatgg tcgaggtctt ccagaaagag gaattctgcc tggagttcgt
     4381 ccaggatttc cttatggggc tgcaggtcat caccattttc ctgatgctga tgatattaga
     4441 aaaactttga aaagattgaa ggcggtgtct aaacaagcca atgcaaacag gcaaaaaggg
     4501 cagctagctg tagaaagagc taaacaagta gaagagttcc tgcagcgaaa acgggaagct
     4561 atgcagaata aagctcgagc cgaaggacat atgggaatcc tgcaaaacct ggcagctatg
     4621 tatggaggca ggcccagctc ttcaagagga gggaagccaa gaaacaaaga ggaagaggtt
     4681 tatctggcaa gactgaggca aataagacta cagaatttca atgagcgcca acagattaaa
     4741 gccaaacttc gtggtgaaaa gaaagaagct aatcattctg aaggacaaga aggaagtgaa
     4801 gaggctgaca tgaggcgcaa aaaaatcgaa tcactgaagg cccatgcaaa tgcacgtgct
     4861 gctgtactaa aagaacaact agaacgaaag agaaaggagg cttatgagag agaaaaaaaa
     4921 gtgtgggaag agcatttggt ggctaaagga gttaagagtt ctgatgtttc tccacctttg
     4981 ggacagcatg aaacaggtgg ctctccatca aagcaacaga tgagatctgt tatttctgta
     5041 acttcagctt tgaaagaagt tggcgtggac agtagtttaa ctgatacccg ggaaacttca
     5101 gaagagatgc aaaagaccaa caatgctatt tcaagtaagc gagaaatact tcgtagatta
     5161 aatgaaaatc ttaaagctca agaagatgaa aaaggaaagc agaatctctc tgatactttt
     5221 gagataaatg ttcatgaaga tgccaaagag catgaaaaag aaaaatcagt ttcatctgat
     5281 cgcaagaagt gggaggcagg aggtcaactt gtgattcctc tggatgagtt aacactagat
     5341 acatccttct ctacaactga aagacataca gtgggagaag ttattaaatt aggtcctaat
     5401 ggatctccaa gaagagcctg ggggaaaagt ccgacagatt ctgttctaaa gatacttgga
     5461 gaagctgaac tacaacttca gacagaacta ttagaaaata caactattag aagtgagatt
     5521 tctcccgaag gggaaaagta caaaccctta attactggag aaaaaaaagt acaatgtatt
     5581 tcacatgaaa taaacccatc agctattgtt gattctcctg ttgagacaaa aagtcccgag
     5641 ttcagtgagg catctccaca gatgtcattg aaactggaag gaaatttaga agaacctgat
     5701 gatttggaaa cagaaattct acaagagcca agtggaacaa acaaagatga gagcttgcca
     5761 tgcactatta ctgatgtgtg gattagtgag gaaaaagaaa caaaggaaac tcagtcggca
     5821 gataggatca ccattcagga aaatgaagtt tctgaagatg gagtctcgag tactgtggac
     5881 caacttagtg acattcatat agagcctgga accaatgatt ctcagcactc taaatgtgat
     5941 gtagataagt ctgtgcaacc ggaaccattt ttccataagg tggttcattc tgaacacttg
     6001 aacttagtcc ctcaagttca atcagttcag tgttcaccag aagaatcctt tgcatttcga
     6061 tctcactcgc atttaccacc aaaaaataaa aacaagaatt ccttgctgat tggactttca
     6121 actggtctgt ttgatgcaaa caacccaaag atgttaagga catgttcact tccagatctc
     6181 tcaaagctgt tcagaaccct tatggatgtt cccaccgtag gagatgttcg tcaagacaat
     6241 cttgaaatag atgaaattga agatgaaaac attaaagaag gaccttctga ttctgaagac
     6301 attgtgtttg aagaaactga cacagattta caagagctgc aggcctcgat ggaacagtta
     6361 cttagggaac aacctggtga agaatacagt gaagaagaag agtcagtctt gaagaacagt
     6421 gatgtggagc caactgcaaa tgggacagat gtggcagatg aagatgacaa tcccagcagt
     6481 gaaagtgccc tgaacgaaga atggcactca gataacagtg atggtgaaat tgctagtgaa
     6541 tgtgaatgcg atagtgtctt taaccattta gaggaactga gacttcatct ggagcaggaa
     6601 atgggctttg aaaaattctt tgaggtttat gagaaaataa aggctattca tgaagatgaa
     6661 gatgaaaata ttgaaatttg ttcaaaaata gttcaaaata ttttgggaaa tgaacatcag
     6721 catctttatg ccaagattct tcatttagtc atggcagatg gagcctacca agaagataat
     6781 gatgaataac tcgagtctag agggccctta gtcgagaatt ctaccgggta ggggaggcgc
     6841 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     6901 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     6961 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     7021 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     7081 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     7141 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     7201 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     7261 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     7321 tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     7381 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     7441 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     7501 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     7561 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     7621 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     7681 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     7741 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     7801 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     7861 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     7921 catgacccgc aagcccggtg cctgaatcga tagatcctaa tcaacctctg gattacaaaa
     7981 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     8041 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     8101 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     8161 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     8221 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     8281 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     8341 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     8401 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     8461 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     8521 ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc
     8581 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     8641 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     8701 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8761 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8821 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     8881 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa
     8941 ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     9001 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     9061 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     9121 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     9181 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     9241 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     9301 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     9361 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     9421 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     9481 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     9541 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     9601 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     9661 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     9721 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     9781 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     9841 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     9901 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     9961 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    10021 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    10081 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    10141 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    10201 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    10261 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    10321 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    10381 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    10441 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    10501 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    10561 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    10621 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    10681 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    10741 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    10801 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    10861 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    10921 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    10981 aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat
    11041 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    11101 aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc
    11161 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    11221 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    11281 aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt
    11341 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    11401 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
    11461 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    11521 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
