LOCUS V036259 11569 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V036259 VERSION V036259 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2058..2175 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" enhancer 2227..2530 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2531..2734 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" regulatory 2815..2824 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 2824..2895 /codon_start=1 /product="three tandem FLAG® epitope tags" /transl_table=1 /translation="DYKDDDDKDYKDDDDKDYKDDDDK" /label="3xFLAG" CDS 2929..6786 /label="NEK1(NM_001199397)" /note="NEK1(NM_001199397)" /gene="NEK1" promoter 6826..7325 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 7346..7945 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7959..8547 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 8622..8855 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" promoter 9060..9164 /gene="bla" /label="AmpR promoter" CDS 9165..10025 /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" rep_origin 10196..10784 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" protein_bind 11195..11216 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" promoter 11231..11261 /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 11269..11285 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" primer_bind 11293..11309 /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga 2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg 2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccac gcgtgccacc 2821 atggattaca aggatgacga cgataaggac tataaggacg atgatgacaa ggactacaaa 2881 gatgatgacg ataaacgaag cccgggcgga tcgggtttaa acggatccat ggagaagtat 2941 gttagactac agaagattgg agaaggttca tttggaaaag ccattcttgt taaatctaca 3001 gaagatggca gacagtatgt tatcaaggaa attaacatct caagaatgtc cagtaaagaa 3061 agagaagaat caaggagaga agttgcagta ttggcaaaca tgaagcatcc aaatattgtc 3121 cagtatagag aatcatttga agaaaatggc tctctctaca tagtaatgga ttactgtgag 3181 ggaggggatc tgtttaagcg aataaatgct cagaaaggcg ttttgtttca agaggatcag 3241 attttggact ggtttgtaca gatatgtttg gccctgaaac atgtacatga tagaaaaatt 3301 cttcatcgag acattaaatc tcagaacata tttttaacta aagatggaac agtacaactt 3361 ggagattttg gaattgctag agttcttaat agtactgtag agctggctcg aacttgcata 3421 gggaccccat actacttgtc acctgaaatc tgtgaaaaca aaccttacaa taataaaagt 3481 gacatttggg ctctggggtg tgtcctttat gagctgtgta cacttaaaca tgcttttgaa 3541 gctggcagta tgaaaaacct ggtactgaag ataatatctg gatcttttcc acctgtgtct 3601 ttgcattatt cctatgatct ccgcagtttg gtgtctcagt tatttaaaag aaatcctagg 3661 gatagaccat cagtcaactc catattggag aaaggtttta tagccaaacg cattgaaaag 3721 tttctctctc ctcagcttat tgcagaagaa ttttgtctaa aaacattttc gaagtttgga 3781 tcacagccta taccagctaa aagaccagct tcaggacaaa actcgatttc tgttatgcct 3841 gctcagaaaa ttacaaagcc tgccgctaaa tatggaatac ctttagcata taagaaatat 3901 ggagataaaa aattacacga aaagaaacca ctgcaaaaac ataaacaggc ccatcaaact 3961 ccagagaaga gagtgaatac tggagaagaa aggaggaaaa tatctgagga agcagcaaga 4021 aagagaaggc tggaatttat tgaaaaagaa aagaaacaaa aggatcagat tattagttta 4081 atgaaggctg aacaaatgaa aaggcaagaa aaggaaaggt tggaaagaat aaatagggcc 4141 agggaacaag gatggagaaa tgtgctaagt gctggtggaa gtggtgaagt aaaggctcct 4201 tttctgggca gtggagggac tatagctcca tcatcttttt cttctcgagg acagtatgaa 4261 cattaccatg ccatttttga ccaaatgcag caacaaagag cagaagataa tgaagctaaa 4321 tggaaaagag aaatatatgg tcgaggtctt ccagaaagag gaattctgcc tggagttcgt 4381 ccaggatttc cttatggggc tgcaggtcat caccattttc ctgatgctga tgatattaga 4441 aaaactttga aaagattgaa ggcggtgtct aaacaagcca atgcaaacag gcaaaaaggg 4501 cagctagctg tagaaagagc taaacaagta gaagagttcc tgcagcgaaa acgggaagct 4561 atgcagaata aagctcgagc cgaaggacat atgggaatcc tgcaaaacct ggcagctatg 4621 tatggaggca ggcccagctc ttcaagagga gggaagccaa gaaacaaaga ggaagaggtt 4681 tatctggcaa gactgaggca aataagacta cagaatttca atgagcgcca acagattaaa 4741 gccaaacttc gtggtgaaaa gaaagaagct aatcattctg aaggacaaga aggaagtgaa 4801 gaggctgaca tgaggcgcaa aaaaatcgaa tcactgaagg cccatgcaaa tgcacgtgct 4861 gctgtactaa aagaacaact agaacgaaag agaaaggagg cttatgagag agaaaaaaaa 4921 gtgtgggaag agcatttggt ggctaaagga gttaagagtt ctgatgtttc tccacctttg 4981 ggacagcatg aaacaggtgg ctctccatca aagcaacaga tgagatctgt tatttctgta 5041 acttcagctt tgaaagaagt tggcgtggac agtagtttaa ctgatacccg ggaaacttca 5101 gaagagatgc aaaagaccaa caatgctatt tcaagtaagc gagaaatact tcgtagatta 5161 aatgaaaatc ttaaagctca agaagatgaa aaaggaaagc agaatctctc tgatactttt 5221 gagataaatg ttcatgaaga tgccaaagag catgaaaaag aaaaatcagt ttcatctgat 5281 cgcaagaagt gggaggcagg aggtcaactt gtgattcctc tggatgagtt aacactagat 5341 acatccttct ctacaactga aagacataca gtgggagaag ttattaaatt aggtcctaat 5401 ggatctccaa gaagagcctg ggggaaaagt ccgacagatt ctgttctaaa gatacttgga 5461 gaagctgaac tacaacttca gacagaacta ttagaaaata caactattag aagtgagatt 5521 tctcccgaag gggaaaagta caaaccctta attactggag aaaaaaaagt acaatgtatt 5581 tcacatgaaa taaacccatc agctattgtt gattctcctg ttgagacaaa aagtcccgag 5641 ttcagtgagg catctccaca gatgtcattg aaactggaag gaaatttaga agaacctgat 5701 gatttggaaa cagaaattct acaagagcca agtggaacaa acaaagatga gagcttgcca 5761 tgcactatta ctgatgtgtg gattagtgag gaaaaagaaa caaaggaaac tcagtcggca 5821 gataggatca ccattcagga aaatgaagtt tctgaagatg gagtctcgag tactgtggac 5881 caacttagtg acattcatat agagcctgga accaatgatt ctcagcactc taaatgtgat 5941 gtagataagt ctgtgcaacc ggaaccattt ttccataagg tggttcattc tgaacacttg 6001 aacttagtcc ctcaagttca atcagttcag tgttcaccag aagaatcctt tgcatttcga 6061 tctcactcgc atttaccacc aaaaaataaa aacaagaatt ccttgctgat tggactttca 6121 actggtctgt ttgatgcaaa caacccaaag atgttaagga catgttcact tccagatctc 6181 tcaaagctgt tcagaaccct tatggatgtt cccaccgtag gagatgttcg tcaagacaat 6241 cttgaaatag atgaaattga agatgaaaac attaaagaag gaccttctga ttctgaagac 6301 attgtgtttg aagaaactga cacagattta caagagctgc aggcctcgat ggaacagtta 6361 cttagggaac aacctggtga agaatacagt gaagaagaag agtcagtctt gaagaacagt 6421 gatgtggagc caactgcaaa tgggacagat gtggcagatg aagatgacaa tcccagcagt 6481 gaaagtgccc tgaacgaaga atggcactca gataacagtg atggtgaaat tgctagtgaa 6541 tgtgaatgcg atagtgtctt taaccattta gaggaactga gacttcatct ggagcaggaa 6601 atgggctttg aaaaattctt tgaggtttat gagaaaataa aggctattca tgaagatgaa 6661 gatgaaaata ttgaaatttg ttcaaaaata gttcaaaata ttttgggaaa tgaacatcag 6721 catctttatg ccaagattct tcatttagtc atggcagatg gagcctacca agaagataat 6781 gatgaataac tcgagtctag agggccctta gtcgagaatt ctaccgggta ggggaggcgc 6841 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca 6901 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt 6961 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg 7021 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg 7081 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat 7141 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg 7201 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat 7261 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc 7321 tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac 7381 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc 7441 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact 7501 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc 7561 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg 7621 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct 7681 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc 7741 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga 7801 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga 7861 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg 7921 catgacccgc aagcccggtg cctgaatcga tagatcctaa tcaacctctg gattacaaaa 7981 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 8041 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 8101 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 8161 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 8221 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 8281 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 8341 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc 8401 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 8461 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 8521 ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc 8581 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc 8641 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 8701 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 8761 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 8821 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 8881 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa 8941 ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg 9001 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc 9061 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 9121 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 9181 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 9241 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 9301 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 9361 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc 9421 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 9481 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 9541 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 9601 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 9661 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa 9721 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 9781 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 9841 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 9901 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 9961 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 10021 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 10081 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 10141 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 10201 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 10261 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 10321 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga 10381 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 10441 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 10501 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 10561 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 10621 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 10681 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 10741 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 10801 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 10861 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 10921 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca 10981 aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat 11041 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 11101 aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc 11161 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg 11221 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat 11281 aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt 11341 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga 11401 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta 11461 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt 11521 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt //