LOCUS 40924_21963 5064 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pGL3-Enhancer, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5064) AUTHORS Groskreutz DJ, Schenborn ET. TITLE Direct Submission JOURNAL Submitted (26-JAN-1996) D.J. Groskreutz, R REFERENCE 2 (bases 1 to 5064) AUTHORS Kenefick K. TITLE Direct Submission JOURNAL Submitted (05-MAR-2001) Technical Writing, Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711-5399, USA REFERENCE 3 (bases 1 to 5064) TITLE Direct Submission REFERENCE 4 (bases 1 to 5064) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (26-JAN-1996) D.J. Groskreutz, R" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (05-MAR-2001) Technical Writing, Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711-5399, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT On Mar 5, 2001 this sequence version replaced U47297.1. FEATURES Location/Qualifiers source 1..5064 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..58 /label=multiple cloning site /note="multiple cloning site" CDS 88..1737 /label=luciferase /note="firefly luciferase" polyA_signal complement(1781..1902) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" regulatory 2013..2249 /regulatory_class="enhancer" primer_bind complement(2307..2326) /label=RV primer4 sequencing primer binding site /note="RV primer4 sequencing primer binding site" rep_origin 2564 /label=ColE1-derived plasmid replication origin /note="ColE1-derived plasmid replication origin" rep_origin complement(2567..3155) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3329..4186) /label=AmpR /note="beta-lactamase" promoter complement(4187..4291) /label=AmpR promoter rep_origin 4318..4773 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" polyA_signal 4904..4952 /label=poly(A) signal /note="synthetic polyadenylation signal" misc_feature 4966..5057 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" ORIGIN 1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg 61 cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa gaaaggcccg 121 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag 181 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc 241 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg 301 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg 361 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa 421 cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag 481 gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc 541 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat 601 ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca 661 attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct 721 catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc 781 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact 841 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag 901 ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta 961 ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa 1021 attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc 1081 catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt 1141 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg 1201 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt 1261 gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg 1321 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac 1381 ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc 1441 gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt 1501 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag 1561 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag 1621 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac 1681 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa 1741 ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt 1801 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct 1861 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt 1921 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc 1981 tacaaatgtg gtaaaatcga taaggatctg aacgatggag cggagaatgg gcggaactgg 2041 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga 2101 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg 2161 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc 2221 cacaccctaa ctgacacaca ttccacagcg gatccgtcga ccgatgccct tgagagcctt 2281 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac 2341 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc 2401 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 2461 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 2521 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 2581 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 2641 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 2701 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 2761 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 2821 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 2881 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 2941 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 3001 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 3061 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 3121 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 3181 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 3241 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 3301 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 3361 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 3421 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 3481 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 3541 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 3601 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 3661 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 3721 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 3781 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 3841 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 3901 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 3961 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 4021 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 4081 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 4141 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 4201 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 4261 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 4321 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 4381 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 4441 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 4501 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 4561 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 4621 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 4681 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 4741 cgaattttaa caaaatatta acgcttacaa tttgccattc gccattcagg ctgcgcaact 4801 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagcccaag ctaccatgat 4861 aagtaagtaa tattaaggta cgggaggtac ttggagcggc cgcaataaaa tatctttatt 4921 ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gtactaacat acgctctcca 4981 tcaaaacaaa acgaaacaaa acaaactagc aaaataggct gtccccagtg caagtgcagg 5041 tgccagaaca tttctctatc gata //