LOCUS       40924_21389        3995 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(88..105)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(873..905)
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     RBS             complement(913..935)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(967..985)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1248..1836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2010..2867)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2868..2972)
                     /label=AmpR promoter
     rep_origin      3306..3761
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     terminator      complement(3860..3907)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3979..3995
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaatactcaa gcttatgcat gcggccgcat ctagagggcc cggatccctc gaggtcgacg
       61 aattcgagct cggccgactt ggccttccct ttagtgaggg ttaataaact tggccaattc
      121 gatccggtac cagcaccacc agcggtgagg tgcggaactt ctacaacctc aaagcccata
      181 acgttgcgga tagaaccctt ctcagggtca atcagagcag cgtagtttgc tgcgttcggc
      241 atcagtgctg ccagaatcgc agagtagcta tctgggtcac agtagaacac acggtcagca
      301 gccggaacat agttcttggt cagagccgca cgagccttag tcagagccgc aataatctcc
      361 ttacccagcg caacttggtc ggtaagtgcg gccttgttct gagtggtctc aattacggta
      421 gcagtaccta agccctcgat gttctcatta tatttgcttt ccacgttaca cagaccggca
      481 atctcagcca gaaccgcacc atccgcagcc atcgccagag attcacccaa ctgagaggta
      541 tactcagagc gaacgtcgta gtggttcatc gcgtcctcaa tatcataaat cagaacgtca
      601 gccgtcagga gaccgtcaat ggtgattacc ttctcggtgt gtttgatgtc cttacgttta
      661 tcgtcgaggt tctcgcccgg agccagatac gctgcctgag tgcgacccag aacagggaac
      721 tgagcggatt taccgctgga gatggaacgt accatgtggc gagaagtggt cacggaggta
      781 cgagcgaacg cagtcaggac ttcaccgcca aataccttca agaacaacgc cagtttatct
      841 ccagcagcaa ctacaccttt accttggtta gtacccattt gctgtccacc agtcatgcta
      901 gccatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg aaaccgttgt
      961 ggtctcccta tagtgagtcg tattaatttc gcgggatcga gatctcccgg gctgcattaa
     1021 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt cgataggctc
     1261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1681 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1921 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1981 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2041 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2101 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2161 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2221 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2281 agttcgccag ttaatagttt gcgcaacgtt gttggcattg ctacaggcat cgtggtgtca
     2341 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2401 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2461 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2521 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2581 gaataccgcg cccggcgacc gagttgctct tgcccggcgt caatacggga taatagtgta
     2641 tgacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2701 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2761 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2821 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2881 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2941 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3001 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     3061 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     3121 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     3181 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     3241 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     3301 aggcgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3361 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3421 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3481 atttagagct ttacggcacc tcgaccgcaa aaaacttgat ttgggtgatg gttcacgtag
     3541 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     3601 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     3661 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaat
     3721 atttaacgcg aattttaaca aaatattaac gtttacaatt tccattcgcc attcaggctg
     3781 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca ggttatccgg
     3841 atatagttcc tcctttcagc aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt
     3901 atgctagtta ttgctcagcg gtggcagcag ccaactcagc ttcctttcgg gctttgttag
     3961 cagccggatc aagctgggat ttaggtgaca ctata
//
