LOCUS       62056_11010        4194 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..242
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             255..523
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     573..632
                     /label=IL-2 signal sequence
                     /note="human interleukin-2 signal sequence"
     polyA_signal    complement(1359..1480)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(1584..1978)
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(1995..2366)
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        complement(2959..3262)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3342..3930)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4038..4086
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4097..4188
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
       61 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
      121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
      301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
      361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
      421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
      481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
      541 ctacctgaga tcaccggcga aggagggcca ccatgtacag gatgcaactc ctgtcttgca
      601 ttgcactaag tcttgcactt gtcacgaatt cgatatcggc catggttaga tctgacaaaa
      661 ctcacacatg cccaccgtgc ccagcacctg aactcctggg gggaccgtca gtcttcctct
      721 tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc acatgcgtgg
      781 tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg gacggcgtgg
      841 aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg taccgtgtgg
      901 tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac aagtgcaagg
      961 tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc aaagggcagc
     1021 cccgagaacc acaggtgtac accctgcccc catcccggga ggagatgacc aagaaccagg
     1081 tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg gagtgggaga
     1141 gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac tccgacggct
     1201 ccttcttcct ctacagcaag ctcaccgtgg acaagagcag gtggcagcag gggaacgtct
     1261 tctcatgctc cgtgatgcac gaggctctgc acaaccacta cacgcagaag agcctctccc
     1321 tgtctccggg taaatgagtg ctagctggcc agacatgata agatacattg atgagtttgg
     1381 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     1441 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     1501 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     1561 caaatgtggt atggaattaa ttctaaaata cagcatagca aaactttaac ctccaaatca
     1621 agcctctact tgaatccttt tctgagggat gaataaggca taggcatcag gggctgttgc
     1681 caatgtgcat tagctgtttg cagcctcacc ttctttcatg gagtttaaga tatagtgtat
     1741 tttcccaagg tttgaactag ctcttcattt ctttatgttt taaatgcact gacctcccac
     1801 attccctttt tagtaaaata ttcagaaata atttaaatac atcattgcaa tgaaaataaa
     1861 tgttttttat taggcagaat ccagatgctc aaggcccttc ataatatccc ccagtttagt
     1921 agttggactt agggaacaaa ggaaccttta atagaaattg gacagcaaga aagcgagctt
     1981 ctagcttatc ctcagtcctg ctcctctgcc acaaagtgca cgcagttgcc ggccgggtcg
     2041 cgcagggcga actcccgccc ccacggctgc tcgccgatct cggtcatggc cggcccggag
     2101 gcgtcccgga agttcgtgga cacgacctcc gaccactcgg cgtacagctc gtccaggccg
     2161 cgcacccaca cccaggccag ggtgttgtcc ggcaccacct ggtcctggac cgcgctgatg
     2221 aacagggtca cgtcgtcccg gaccacaccg gcgaagtcgt cctccacgaa gtcccgggag
     2281 aacccgagcc ggtcggtcca gaactcgacc gctccggcga cgtcgcgcgc ggtgagcacc
     2341 ggaacggcac tggtcaactt ggccatgatg gctcctcctg tcaggagagg aaagagaaga
     2401 aggttagtac aattgctata gtgagttgta ttatactatg cagatatact atgccaatga
     2461 ttaattgtca aactagggct gcagggttca tagtgccact tttcctgcac tgccccatct
     2521 cctgcccacc ctttcccagg catagacagt cagtgactta ccaaactcac aggagggaga
     2581 aggcagaagc ttgagacaga cccgcgggac cgccgaactg cgaggggacg tggctagggc
     2641 ggcttctttt atggtgcgcc ggccctcgga ggcagggcgc tcggggaggc ctagcggcca
     2701 atctgcggtg gcaggaggcg gggccgaagg ccgtgcctga ccaatccgga gcacatagga
     2761 gtctcagccc cccgccccaa agcaagggga agtcacgcgc ctgtagcgcc agcgtgttgt
     2821 gaaatggggg cttggggggg ttggggccct gactagtcaa aacaaactcc cattgacgtc
     2881 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
     2941 ctgccaaaac cgcatcatca tggtaatagc gatgactaat acgtagatgt actgccaagt
     3001 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     3061 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     3121 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     3181 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     3241 atttaccgta agttatgtaa cgcctgcagg ttaattaaga acatgtgagc aaaaggccag
     3301 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3361 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3421 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3481 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3541 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3601 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3661 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3721 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3781 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3841 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3901 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3961 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatggctag ttaattaaca
     4021 tttaaatcag cggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt
     4081 tgtgtgaatc gtaactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag
     4141 caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctat cgaa
//
