LOCUS       62056_11000        4417 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..242
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             255..523
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     polyA_signal    complement(1582..1703)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(1807..2201)
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(2218..2589)
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        complement(3182..3485)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3565..4153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4261..4309
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4320..4411
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
       61 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
      121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
      301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
      361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
      421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
      481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
      541 ctacctgaga tcaccggtga attcgatatc tcgagtgcta gcaccaaggg cccatcggtc
      601 ttccccctgg cgccctgctc caggagcacc tccgagagca cagccgccct gggctgcctg
      661 gtcaaggact acttccccga accggtgacg gtgtcgtgga actcaggcgc cctgaccagc
      721 ggcgtgcaca ccttcccggc tgtcctacag tcctcaggac tctactccct cagcagcgtg
      781 gtgaccgtgc cctccagcag cttgggcacg aagacctaca cctgcaacgt agatcacaag
      841 cccagcaaca ccaaggtgga caagagagtt gagtccaaat atggtccccc atgcccatca
      901 tgcccagcac ctgagttcct ggggggacca tcagtcttcc tgttcccccc aaaacccaag
      961 gacactctca tgatctcccg gacccctgag gtcacgtgcg tggtggtgga cgtgagccag
     1021 gaagaccccg aggtccagtt caactggtac gtggatggcg tggaggtgca taatgccaag
     1081 acaaagccgc gggaggagca gttcaacagc acgtaccgtg tggtcagcgt cctcaccgtc
     1141 ctgcaccagg actggctgaa cggcaaggag tacaagtgca aggtctccaa caaaggcctc
     1201 ccgtcctcca tcgagaaaac catctccaaa gccaaagggc agccccgaga gccacaggtg
     1261 tacaccctgc ccccatccca ggaggagatg accaagaacc aggtcagcct gacctgcctg
     1321 gtcaaaggct tctaccccag cgacatcgcc gtggagtggg agagcaatgg gcagccggag
     1381 aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt cctctacagc
     1441 aggctcaccg tggacaagag caggtggcag gaggggaatg tcttctcatg ctccgtgatg
     1501 catgaggctc tgcacaacca ctacacacag aagagcctct ccctgtctcc gggtaaatga
     1561 gtcctagctg gccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1621 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1681 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1741 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggaat
     1801 taattctaaa atacagcata gcaaaacttt aacctccaaa tcaagcctct acttgaatcc
     1861 ttttctgagg gatgaataag gcataggcat caggggctgt tgccaatgtg cattagctgt
     1921 ttgcagcctc accttctttc atggagttta agatatagtg tattttccca aggtttgaac
     1981 tagctcttca tttctttatg ttttaaatgc actgacctcc cacattccct ttttagtaaa
     2041 atattcagaa ataatttaaa tacatcattg caatgaaaat aaatgttttt tattaggcag
     2101 aatccagatg ctcaaggccc ttcataatat cccccagttt agtagttgga cttagggaac
     2161 aaaggaacct ttaatagaaa ttggacagca agaaagcgag cttctagctt atcctcagtc
     2221 ctgctcctct gccacaaagt gcacgcagtt gccggccggg tcgcgcaggg cgaactcccg
     2281 cccccacggc tgctcgccga tctcggtcat ggccggcccg gaggcgtccc ggaagttcgt
     2341 ggacacgacc tccgaccact cggcgtacag ctcgtccagg ccgcgcaccc acacccaggc
     2401 cagggtgttg tccggcacca cctggtcctg gaccgcgctg atgaacaggg tcacgtcgtc
     2461 ccggaccaca ccggcgaagt cgtcctccac gaagtcccgg gagaacccga gccggtcggt
     2521 ccagaactcg accgctccgg cgacgtcgcg cgcggtgagc accggaacgg cactggtcaa
     2581 cttggccatg atggctcctc ctgtcaggag aggaaagaga agaaggttag tacaattgct
     2641 atagtgagtt gtattatact atgcagatat actatgccaa tgattaattg tcaaactagg
     2701 gctgcagggt tcatagtgcc acttttcctg cactgcccca tctcctgccc accctttccc
     2761 aggcatagac agtcagtgac ttaccaaact cacaggaggg agaaggcaga agcttgagac
     2821 agacccgcgg gaccgccgaa ctgcgagggg acgtggctag ggcggcttct tttatggtgc
     2881 gccggccctc ggaggcaggg cgctcgggga ggcctagcgg ccaatctgcg gtggcaggag
     2941 gcggggccga aggccgtgcc tgaccaatcc ggagcacata ggagtctcag ccccccgccc
     3001 caaagcaagg ggaagtcacg cgcctgtagc gccagcgtgt tgtgaaatgg gggcttgggg
     3061 gggttggggc cctgactagt caaaacaaac tcccattgac gtcaatgggg tggagacttg
     3121 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     3181 tcatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     3241 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     3301 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     3361 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     3421 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     3481 taacgcctgc aggttaatta agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3541 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3601 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3661 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3721 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3781 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3841 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3901 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3961 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4021 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4081 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4141 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4201 aaactcacgt taagggattt tggtcatggc tagttaatta acatttaaat cagcggccgc
     4261 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgtaacta
     4321 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
     4381 agtgcaagtg caggtgccag aacatttctc tatcgaa
//
