LOCUS 62056_11000 4417 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4417) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..4417 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 31..242 /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" LTR 255..523 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" polyA_signal complement(1582..1703) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" polyA_signal complement(1807..2201) /label=beta-globin poly(A) /note="human beta-globin polyadenylation signal" CDS complement(2218..2589) /codon_start=1 /label=BleoR /note="antibiotic-binding protein" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" enhancer complement(3182..3485) /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" rep_origin complement(3565..4153) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" polyA_signal 4261..4309 /label=poly(A) signal /note="synthetic polyadenylation signal" misc_feature 4320..4411 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" ORIGIN 1 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg 61 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa 121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt 181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac 241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc 301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg 361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc 421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac 481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc 541 ctacctgaga tcaccggtga attcgatatc tcgagtgcta gcaccaaggg cccatcggtc 601 ttccccctgg cgccctgctc caggagcacc tccgagagca cagccgccct gggctgcctg 661 gtcaaggact acttccccga accggtgacg gtgtcgtgga actcaggcgc cctgaccagc 721 ggcgtgcaca ccttcccggc tgtcctacag tcctcaggac tctactccct cagcagcgtg 781 gtgaccgtgc cctccagcag cttgggcacg aagacctaca cctgcaacgt agatcacaag 841 cccagcaaca ccaaggtgga caagagagtt gagtccaaat atggtccccc atgcccatca 901 tgcccagcac ctgagttcct ggggggacca tcagtcttcc tgttcccccc aaaacccaag 961 gacactctca tgatctcccg gacccctgag gtcacgtgcg tggtggtgga cgtgagccag 1021 gaagaccccg aggtccagtt caactggtac gtggatggcg tggaggtgca taatgccaag 1081 acaaagccgc gggaggagca gttcaacagc acgtaccgtg tggtcagcgt cctcaccgtc 1141 ctgcaccagg actggctgaa cggcaaggag tacaagtgca aggtctccaa caaaggcctc 1201 ccgtcctcca tcgagaaaac catctccaaa gccaaagggc agccccgaga gccacaggtg 1261 tacaccctgc ccccatccca ggaggagatg accaagaacc aggtcagcct gacctgcctg 1321 gtcaaaggct tctaccccag cgacatcgcc gtggagtggg agagcaatgg gcagccggag 1381 aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt cctctacagc 1441 aggctcaccg tggacaagag caggtggcag gaggggaatg tcttctcatg ctccgtgatg 1501 catgaggctc tgcacaacca ctacacacag aagagcctct ccctgtctcc gggtaaatga 1561 gtcctagctg gccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa 1621 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca 1681 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc 1741 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggaat 1801 taattctaaa atacagcata gcaaaacttt aacctccaaa tcaagcctct acttgaatcc 1861 ttttctgagg gatgaataag gcataggcat caggggctgt tgccaatgtg cattagctgt 1921 ttgcagcctc accttctttc atggagttta agatatagtg tattttccca aggtttgaac 1981 tagctcttca tttctttatg ttttaaatgc actgacctcc cacattccct ttttagtaaa 2041 atattcagaa ataatttaaa tacatcattg caatgaaaat aaatgttttt tattaggcag 2101 aatccagatg ctcaaggccc ttcataatat cccccagttt agtagttgga cttagggaac 2161 aaaggaacct ttaatagaaa ttggacagca agaaagcgag cttctagctt atcctcagtc 2221 ctgctcctct gccacaaagt gcacgcagtt gccggccggg tcgcgcaggg cgaactcccg 2281 cccccacggc tgctcgccga tctcggtcat ggccggcccg gaggcgtccc ggaagttcgt 2341 ggacacgacc tccgaccact cggcgtacag ctcgtccagg ccgcgcaccc acacccaggc 2401 cagggtgttg tccggcacca cctggtcctg gaccgcgctg atgaacaggg tcacgtcgtc 2461 ccggaccaca ccggcgaagt cgtcctccac gaagtcccgg gagaacccga gccggtcggt 2521 ccagaactcg accgctccgg cgacgtcgcg cgcggtgagc accggaacgg cactggtcaa 2581 cttggccatg atggctcctc ctgtcaggag aggaaagaga agaaggttag tacaattgct 2641 atagtgagtt gtattatact atgcagatat actatgccaa tgattaattg tcaaactagg 2701 gctgcagggt tcatagtgcc acttttcctg cactgcccca tctcctgccc accctttccc 2761 aggcatagac agtcagtgac ttaccaaact cacaggaggg agaaggcaga agcttgagac 2821 agacccgcgg gaccgccgaa ctgcgagggg acgtggctag ggcggcttct tttatggtgc 2881 gccggccctc ggaggcaggg cgctcgggga ggcctagcgg ccaatctgcg gtggcaggag 2941 gcggggccga aggccgtgcc tgaccaatcc ggagcacata ggagtctcag ccccccgccc 3001 caaagcaagg ggaagtcacg cgcctgtagc gccagcgtgt tgtgaaatgg gggcttgggg 3061 gggttggggc cctgactagt caaaacaaac tcccattgac gtcaatgggg tggagacttg 3121 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca 3181 tcatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg 3241 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg 3301 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca 3361 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg 3421 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg 3481 taacgcctgc aggttaatta agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 3541 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 3601 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 3661 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 3721 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 3781 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 3841 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 3901 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 3961 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 4021 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 4081 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 4141 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 4201 aaactcacgt taagggattt tggtcatggc tagttaatta acatttaaat cagcggccgc 4261 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgtaacta 4321 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc 4381 agtgcaagtg caggtgccag aacatttctc tatcgaa //