LOCUS       Exported                5748 bp DNA     circular SYN 19-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          998..1930
                     /plasmid="pFR-Luc"
                     /lab_host="Escherichia coli K12"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:75696"
                     /organism="Cloning vector pFR-Luc"
     promoter        32..136
                     /gene="bla"
                     /label=AmpR promoter
     CDS             137..997
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      998..1930
                     /label=Col EI origin
                     /note="Col EI origin"
     misc_feature    1934..2074
                     /label=bom
                     /note="basis of mobility region from pBR322"
     polyA_signal    2313..2445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2539..2671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2693..2787
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             2868..4520
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     intron          4651..4716
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    5286..5420
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
     1561 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1741 gcggagctat ggaaaaacgc cagcaacgcg cctttttacg gttcctggcc ttttgctggc
     1801 cttttgctca catgttcttt cctgcgttat ccctgattct gtggataacc gtattaccgc
     1861 ctttgagtgc tgataccgct cgccgcagcc gaacgaccga gcgcaagtca gtgagcgagg
     1921 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     1981 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     2041 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     2101 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2161 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggatca
     2221 gccatatcac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2281 acctgaaaca taaaatgaat gcaattgttg ttaacttgtt tattgcagct tataatggtt
     2341 acaaataaag caatagcatc acaaatttca caaataaagc atttttcact gcattctagt
     2401 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcagcca tatcacattt
     2461 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     2521 atgaatgcaa ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2581 agcatcacaa atttcacaaa taaagcaatt tttcactgca ttctattgtg gtttgtccaa
     2641 actcatcaat gtatcttatc atgtctggat ccaagcttgc atgcctgcag gtcggagtac
     2701 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt
     2761 cctccgagcg gagtactgtc ctccgagcgg agactctaga gggtatataa tggatccccg
     2821 ggtaccgagc tcgaattcca gcttggcatt ccggtactgt tggtaaaatg gaagacgcca
     2881 aaaacataaa gaaaggcccg gcgccattct atcctctaga ggatggaacc gctggagagc
     2941 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg
     3001 cacatatcga ggtgaacatc acgtacgcgg aatacttcga aatgtccgtt cggttggcag
     3061 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
     3121 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
     3181 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gaacatttcg cagcctaccg
     3241 tagtgtttgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aaattaccaa
     3301 taatccagaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt
     3361 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtaccagagt
     3421 cctttgatcg tgacaaaaca attgcactga taatgaattc ctctggatct actgggttac
     3481 ctaagggtgt ggcccttccg catagaactg cctgcgtcag attctcgcat gccagagatc
     3541 ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc
     3601 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa
     3661 tgtatagatt tgaagaagag ctgtttttac gatcccttca ggattacaaa attcaaagtg
     3721 cgttgctagt accaacccta ttttcattct tcgccaaaag cactctgatt gacaaatacg
     3781 atttatctaa tttacacgaa attgcttctg ggggcgcacc tctttcgaaa gaagtcgggg
     3841 aagcggttgc aaaacgcttc catcttccag ggatacgaca aggatatggg ctcactgaga
     3901 ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag
     3961 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta
     4021 atcagagagg cgaattatgt gtcagaggac ctatgattat gtccggttat gtaaacaatc
     4081 cggaagcgac caacgccttg attgacaagg atggatggct acattctgga gacatagctt
     4141 actgggacga agacgaacac ttcttcatag ttgaccgctt gaagtcttta attaaataca
     4201 aaggatatca ggtggccccc gctgaattgg aatcgatatt gttacaacac cccaacatct
     4261 tcgacgcggg cgtggcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg
     4321 ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc
     4381 aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag
     4441 gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg
     4501 gcggaaagtc caaattgtaa aatgtaactg tattcagcga tgacgaaatt cttagctatt
     4561 gtaatactct agaggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     4621 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     4681 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aacttatgaa
     4741 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     4801 tctagtgatg atgaggctac tgctgactct caacattcta ctctcaaaag aagagaaagg
     4861 tagagaccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
     4921 aatagaactc ttgcttgctt tgctatttac aaccacaaag gaaaaagctg cactgctata
     4981 caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca
     5041 taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc
     5101 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat
     5161 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg
     5221 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     5281 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5341 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5401 tatcttatca tgtctggatc cccaggaagc tcctctgtgt cctcataaac cctaacctcc
     5461 tctacttgag aggacattcc aatcataggc tgcccatcca ccctctgtgt cctcctgtta
     5521 attaggtcac ttaacaaaaa ggaaattggg taggggtttt tcacagaccg ctttctaagg
     5581 ggtaatttta aaatatctgg gaagtccctt ccactgctgt gttccagaag tgttggtaaa
     5641 cagcccacaa atgtcaacag cagaaacata caagctgtca ctttgcacaa agggcctcgt
     5701 gatacgccta tttttatagg ttaatgtcat gataataatg gtttctta
//
