LOCUS       V006294                 9033 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006294
VERSION     V006294
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9033)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9033)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9033)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9033)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      169..760
                     /label="5' MMLV-LTR"
                     /note="5' MMLV-LTR"
                     /regulatory_class="promoter"
     LTR             289..758
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    823..1180
                     /label="MMLV Psi"
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1245..1661
                     /label="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1671..2045
                     /label="pol region"
                     /note="Moloney murine leukemia virus (MMLV) pol region
                     containing the splice acceptor site"
     misc_feature    2077..2115
                     /label="E-tag"
                     /note="E-tag"
     CDS             2125..4494
                     /gene="TNFAIP3"
                     /label="Tumor necrosis factor alpha-induced protein 3"
                     /note="Tumor necrosis factor alpha-induced protein 3 from
                     Homo sapiens. Accession#: P21580"
     misc_feature    4498..4541
                     /label="3' UTR of hA20"
                     /note="3' UTR of hA20"
     misc_feature    4591..5053
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             5058..5087
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             5192..5209
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             5225..6016
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     LTR             6079..6672
                     /label="LTR"
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    6883..7023
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7209..7797)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7971..8828)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8829..8933)
                     /label="AmpR promoter"
ORIGIN
        1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
       61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
      121 ccctattccc cacactcacc ggagccaaag ccgcgggaca tatacatgtg aaagacccca
      181 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat
      241 aactgagaat agaaaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc
      301 caaagcggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa
      361 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
      421 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga
      481 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
      541 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
      601 aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc
      661 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
      721 ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg
      781 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta
      841 tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta
      901 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc
      961 cggccgcaac cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg
     1021 agtccaaaaa tcccgatcgt tttggactct ttggtgcacc ccccttagag gagggatatg
     1081 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
     1141 cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc
     1201 tctgtctgac tgtgtttctg tatttgtctg aaaatatggg cccgggccag actgttacca
     1261 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcagatcgct cacaaccagt
     1321 cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta
     1381 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca
     1441 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg
     1501 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc
     1561 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc
     1621 gatcctccct ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga
     1681 tcttatatgg ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta
     1741 ctaacagccc ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct
     1801 ggagacctct ggcggcagcc taccaagaac aactggaccg accggtggta cctcaccctt
     1861 accgagtcgg cgacacagtg tgggtccgcc gacaccagac taagaaccta gaacctcgct
     1921 ggaaaggacc ttacacagtc ctgctgacca cccccaccgc cctcaaagta gacggcatcg
     1981 cagcttggat acacgccgcc cacgtgaagg ctgccgaccc cgggggtgga ccatcctcta
     2041 gactgccgga tcgaattgtc gacgaattcc accatgggtg cgccggtgcc gtatccggac
     2101 ccgctggaac cgcgtgcggc cgcaatggct gaacaagtcc ttcctcaggc tttgtatttg
     2161 agcaatatgc ggaaagctgt gaagatacgg gagagaactc cagaagacat ttttaaacct
     2221 actaatggga tcattcatca ttttaaaacc atgcaccgat acacactgga aatgttcaga
     2281 acttgccagt tttgtcctca gtttcgggag atcatccaca aagccctcat cgacagaaac
     2341 atccaggcca ccctggaaag ccagaagaaa ctcaactggt gtcgagaagt ccggaagctt
     2401 gtggcgctga aaacgaacgg tgacggcaat tgcctcatgc atgccacttc tcagtacatg
     2461 tggggcgttc aggacacaga cttggtactg aggaaggcgc tgttcagcac gctcaaggaa
     2521 acagacacac gcaactttaa attccgctgg caactggagt ctctcaaatc tcaggaattt
     2581 gttgaaacgg ggctttgcta tgatactcgg aactggaatg atgaatggga caatcttatc
     2641 aaaatggctt ccacagacac acccatggcc cgaagtggac ttcagtacaa ctcactggaa
     2701 gaaatacaca tatttgtcct ttgcaacatc ctcagaaggc caatcattgt catttcagac
     2761 aaaatgctaa gaagtttgga atcaggttcc aatttcgccc ctttgaaagt gggtggaatt
     2821 tacttgcctc tccactggcc tgcccaggaa tgctacagat accccattgt tctcggctat
     2881 gacagccatc attttgtacc cttggtgacc ctgaaggaca gtgggcctga aatccgagct
     2941 gttccacttg ttaacagaga ccggggaaga tttgaagact taaaagttca ctttttgaca
     3001 gatcctgaaa atgagatgaa ggagaagctc ttaaaagagt acttaatggt gatagaaatc
     3061 cccgtccaag gctgggacca tggcacaact catctcatca atgccgcaaa gttggatgaa
     3121 gctaacttac caaaagaaat caatctggta gatgattact ttgaacttgt tcagcatgag
     3181 tacaagaaat ggcaggaaaa cagcgagcag gggaggagag aggggcacgc ccagaatccc
     3241 atggaacctt ccgtgcccca gctttctctc atggatgtaa aatgtgaaac gcccaactgc
     3301 cccttcttca tgtctgtgaa cacccagcct ttatgccatg agtgctcaga gaggcggcaa
     3361 aagaatcaaa acaaactccc aaagctgaac tccaagccgg gccctgaggg gctccctggc
     3421 atggcgctcg gggcctctcg gggagaagcc tatgagccct tggcgtggaa ccctgaggag
     3481 tccactgggg ggcctcattc ggccccaccg acagcaccca gcccttttct gttcagtgag
     3541 accactgcca tgaagtgcag gagccccggc tgccccttca cactgaatgt gcagcacaac
     3601 ggattttgtg aacgttgcca caacgcccgg caacttcacg ccagccacgc cccagaccac
     3661 acaaggcact tggatcccgg gaagtgccaa gcctgcctcc aggatgttac caggacattt
     3721 aatgggatct gcagtacttg cttcaaaagg actacagcag aggcctcctc cagcctcagc
     3781 accagcctcc ctccttcctg tcaccagcgt tccaagtcag atccctcgcg gctcgtccgg
     3841 agcccctccc cgcattcttg ccacagagct ggaaacgacg cccctgctgg ctgcctgtct
     3901 caagctgcac ggactcctgg ggacaggacg gggacgagca agtgcagaaa agccggctgc
     3961 gtgtattttg ggactccaga aaacaagggc ttttgcacac tgtgtttcat cgagtacaga
     4021 gaaaacaaac attttgctgc tgcctcaggg aaagtcagtc ccacagcgtc caggttccag
     4081 aacaccattc cgtgcctggg gagggaatgc ggcacccttg gaagcaccat gtttgaagga
     4141 tactgccaga agtgtttcat tgaagctcag aatcagagat ttcatgaggc caaaaggaca
     4201 gaagagcaac tgagatcgag ccagcgcaga gatgtgcctc gaaccacaca aagcacctca
     4261 aggcccaagt gcgcccgggc ctcctgcaag aacatcctgg cctgccgcag cgaggagctc
     4321 tgcatggagt gtcagcatcc caaccagagg atgggccctg gggcccaccg gggtgagcct
     4381 gcccccgaag acccccccaa gcagcgttgc cgggcccccg cctgtgatca ttttggcaat
     4441 gccaagtgca acggctactg caacgaatgc tttcagttca agcagatgta tggctaaccg
     4501 gaaacaggtg ggtcacctcc tgcaagaagt ggggcctcga gcggccgcga tccggttatt
     4561 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     4621 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     4681 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     4741 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     4801 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     4861 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     4921 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     4981 gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     5041 acacgatcga taatatggaa caaaaactta tttctgaaga agacttggac accaaacttt
     5101 cctgccgctc gatttctcca cccaggtgaa ctcctccctc acctccccga cggggcgagg
     5161 ctccatggct gccccctcgc tgcacccgtc cctggtgcca cgcggttcca tgggatcgtt
     5221 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     5281 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     5341 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     5401 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     5461 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     5521 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     5581 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     5641 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     5701 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     5761 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     5821 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     5881 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     5941 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     6001 tcttgacgag ttcttctgag cgggactctg gggttcgata aaataaaaga ttttatttag
     6061 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     6121 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag
     6181 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     6241 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
     6301 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     6361 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     6421 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     6481 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
     6541 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     6601 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     6661 gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc
     6721 caccaccggg aggtaagctg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     6781 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     6841 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     6901 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
     6961 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     7021 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7081 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7141 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7201 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7261 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7321 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7381 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7441 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7501 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7561 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7621 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     7681 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7741 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7801 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7861 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7921 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7981 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8041 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8101 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8161 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8221 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8281 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8341 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8401 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8461 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8521 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8581 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8641 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8701 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8761 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8821 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8881 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8941 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     9001 aataggcgta tcacgaggcc ctttcgtctt caa
//
