LOCUS       V040603                13593 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040603
VERSION     V040603
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..8273
                     /label="ZNF462(NM_021224)"
                     /note="ZNF462(NM_021224)"
                     /gene="ZNF462"
     CDS             8280..8345
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    8369..8957
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    9023..9071
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      9273..9701
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9715..10044
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             10111..10905
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    11081..11202
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(11251..11267)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11275..11291
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11299..11329)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11344..11365
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11653..12241)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12412..13272)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13273..13377)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgga ggtgcttcag tgtgatggct
      781 gtgatttccg agccccgtct tatgaagatc tcaaggcaca cattcaggat gtccacacgg
      841 catttctgca gccaactgat gttgctgagg acaatgtgaa tgagctacga tgtgggtccg
      901 tgaatgccag taatcagaca gaggtggagt tttcttctat aaaggatgaa tttgccattg
      961 cagaagattt atcaggtcaa aatgcaactt cattggggac cggaggttac tatggccaca
     1021 gtccaggata ttatggtcag catattgccg ctaatcccaa accaacaaac aagttttttc
     1081 aatgcaagtt ctgtgtacgc tacttcaggt caaaaaacct cctcatagaa cacactagaa
     1141 aggtccatgg agctcaagct gaagggagtt catcaggacc ccctgtcccg ggatccttaa
     1201 attataatat catgatgcac gagggatttg gaaaggtctt ctcttgccag ttttgcacat
     1261 acaagtcacc aagaagggca agaataatta agcatcagaa gatgtatcac aaaaacaatt
     1321 tgaaggagac cactgctccc ccacctgctc ctgctccaat gccagaccct gtggttccgc
     1381 ccgtatcact gcaggacccc tgcaaggaac tgccagcaga ggttgtggag cgcagcatct
     1441 tagagtctat ggtcaagcct ttgaccaaat ctcgaggcaa cttttgttgt gagtggtgca
     1501 gctaccagac cccccgccga gaacgctggt gtgaccacat gatgaagaaa caccgcagta
     1561 tggtcaagat cctttccagt ctcagacagc aacaagaagg aactaatcta cctgatgtgc
     1621 cgaacaagag tgcccccagc cccacttcca actccaccta tctgaccatg aatgctgcaa
     1681 gccgggagat acccaatact accgtctcca acttcagggg ctccatgggc aactccatca
     1741 tgagacccaa ttcttcagct tccaagtttt cgcccatgtc ttaccctcag atgaagccga
     1801 agtcacctca caattctggt ctagttaact tgacagagag atcccgttat ggaatgactg
     1861 acatgaccaa ttcttctgct gacctggaaa ctaacagcat gctaaatgac tctagttctg
     1921 atgaagagtt aaatgaaata gacagtgaga atggtttaag tgctatggat caccagacat
     1981 caggcctgtc tgcagagcag ctgatgggct cagatggcaa caaattattg gagaccaagg
     2041 ggattccatt tagaagattc atgaataggt tccagtgccc cttttgtcct ttcctcacca
     2101 tgcatcgacg tagcatctct cgtcacatag aaaacatcca cttatctgga aagacagctg
     2161 tctacaaatg tgacgaatgt ccgtttactt gcaagagctc gttgaaactt ggggctcaca
     2221 aacagtgtca cacgggtaca acgtcagatt gggatgctgt gaattcccag agtgaaagca
     2281 tttcttcctc actgaatgaa ggtgtggtgt cttatgagag ctcaagcatc aatggtagaa
     2341 agtcaggagt catgttggat cccttgcagc agcaacagcc accgcagcca ccaccaccgc
     2401 cgccgccacc accaccatca cagccacagc cactgcagca gccacagcca ccacagctgc
     2461 agccaccaca tcaggtgcca ccccagccac aaacacagcc accaccaacg cagcagccac
     2521 agccacccac acaagccgca cctctgcacc catacaaatg caccatgtgt aattactcca
     2581 ccacaactct gaaagggcta agagtccatc agcagcataa acattcattc tgtgacaact
     2641 tgccaaaatt cgaggggcag ccctcaagcc taccattgga aaatgagaca gacagccacc
     2701 cctcttccag caacactgtg aagaaaagtc agacctcaat tcttgggttg tcctccaaga
     2761 acaattttgt agctaaagcc tctaggaagc tcgccaatga ctttcctcta gatttgtcac
     2821 ccgtgaagaa gagaaccagg attgacgaga tagcaagcaa ccttcagagc aaaattaacc
     2881 aaaccaaaca gcaggaagat gcagtgatca atgttgagga tgatgaagag gaagaggaag
     2941 acaacgaagt cgagatagag gttgagttgg acagggagga agaaccgaca gaacccatca
     3001 tagaggttcc cacttccttt tctgcccaac agatatgggt aagagatacc agtgagcccc
     3061 agaaagagcc caacttcaga aacatcaccc acgattacaa tgccaccaat ggggctgaga
     3121 ttgagctcac cctttctgaa gatgaagagg attattatgg ctcctcaaca aacttgaaag
     3181 atcaccaagt ttccaatact gctctgctga atacccaaac tcccatctat gggactgagc
     3241 acaatagtga aaacacagac tttggtgact ctggaaggct ttactattgt aaacactgtg
     3301 actttaacaa caaatctgcc cggagtgtta gcacccacta ccaacgaatg cacccataca
     3361 ttaaattcag ctttaggtac atcttggacc ccaatgatca cagtgcagtg tacaggtgcc
     3421 tggaatgcta catcgattac accaacttcg aagatctcca gcagcattat ggcgagcacc
     3481 acccagaagc catgaatgta ctcaactttg atcactcgga cctgatctac cggtgtcggt
     3541 tttgttcata cacgagcccg aatgttagaa gcctgatgcc acattaccaa agaatgcatc
     3601 ccacggtgaa gatcaacaac gcgatgatat tttcaagcta tgtcgtggag cagcaggaag
     3661 ggctgaatac agaatcccag accctgaggg agattctgaa ttcggctccc aagaacatgg
     3721 cgacttccac acctgtggct cgtggtggtg gtttgccagc tacgttcaac aaaaacactc
     3781 ctaagacctt tactcctgaa tgtgaaaatc agaaggaccc tttggtcaac actgttgttg
     3841 tttatgattg tgatgtttgt tcgtttgcaa gccccaacat gcattctgtc ttggttcatt
     3901 atcagaagaa acaccccgaa gaaaaggctt cctactttag gatccagaaa actatgcgaa
     3961 tggtgtctgt ggacaggggc tctgcccttt ctcaattatc atttgaggtg ggtgctccaa
     4021 tgtctcccaa aatgtccaac atgggttccc cacccccccc acaacccccg ccaccagacc
     4081 tcagtactga gctttactac tgcaaacact gttcctacag caatcggtca gttgtgggag
     4141 tgcttgtcca ctaccagaaa agacacccag aaataaaggt tactgccaaa tatatcagac
     4201 aggctcctcc cacagctgca atgatgagag gggtcgaagg gccccaaggc tccccccggc
     4261 cacccgcccc catacaacag ctgaaccgaa gcagctctga gagagatggc cctcctgtgg
     4321 agaatgagat gttcttttgc cagcactgtg attatgggaa ccggacggtc aaaggggtac
     4381 tcattcatta tcagaagaag caccgagact tcaaggccaa tgcagatgtg atccggcagc
     4441 atacggccac cattcgaagc ctctgcgacc gaaatcagaa gaagcctgcc agctgcgtgc
     4501 ttgtctcccc ctctaatctg gagcgggaca aaacgaaact ccgagcactc aaatgtaggc
     4561 agtgctcata tacctccccc tacttctatg cactgaggaa gcatatcaag aaagaccacc
     4621 ccgccctgaa agccacagtc acgtccatca tgcgatgggc atttctagat ggcttgatag
     4681 aagctggcta ccactgcgag tggtgcatct actcccatac ggagcccaac ggtttgctcc
     4741 tgcattacca acggaggcat ccagaacact atgttgatta cacctacatg gctactaaac
     4801 tgtgggctgg gccagaccca tcccctccct ctctcacaat gccagccgaa gccaaaacct
     4861 acagatgcag ggactgtgtt ttcgaagctg tttccatctg ggacatcact aatcactacc
     4921 aagcattcca cccctgggcc atgaatggtg atgagtcagt gctactggac atcatcaagg
     4981 agaaagatgc tgtggagaag cccattcttt catccgaaga gttggcaggc cctgtgaatt
     5041 gtgaaaacag tatacccacc cctttcccgg agcaggaagc tgaatgtcca gaggatgcaa
     5101 gactgtcccc tgagaaaagc ctgcagctag cttcagccaa ccccgccata tcctccaccc
     5161 cataccagtg cacggtatgc caatctgagt ataacaactt gcacggcctt ctcactcatt
     5221 atgggaagaa gcaccctggc atgaaagtga aggctgctga ctttgcccag gacattgaca
     5281 tcaacccagg tgccgtctac aaatgcaggc attgcccata catcaacacc cgcatccacg
     5341 gcgtactgac ccactaccag aagcgacacc cgtccatcaa ggtgaccgct gaggactttg
     5401 tgcacgacgt agagcagtct gctgacatat cccagaatga cgtggaggag acgagcagga
     5461 tcttcaagca agggtatggc gcctaccggt gcaaactgtg tccgtacaca cacggcactt
     5521 tggagaaact aaaaatccac tacgagaagt atcacaatca gcctgaattt gatgtctttt
     5581 cccagtcgcc cccgaagctg ccagtccccc tcgagcccga gatgaccact gaagtgagcc
     5641 cttcccaagt ctccatcact gaggaggagg tgggagagga gcccgtgtcc acttctcact
     5701 tctctacctc ccacctggtc tcccacactg tgttccggtg ccagctctgc aagtacttct
     5761 gctccacgag gaaggggatc gccaggcact accgcatcaa gcacaataat gtccgagccc
     5821 agccagaagg caagaacaac ctcttcaagt gtgccctgtg tgcctacacc aaccccatcc
     5881 gcaaaggtct ggcagcccac taccagaagc gccacgacat tgatgcgtat tacactcact
     5941 gcttggcagc ctccaggacc atcagcgaca agcccaacaa agtgatcatc ccatccccgc
     6001 ccaaggacga ctcccctcag ctgagcgagg aactccggcg ggcagtggag aagaaaaagt
     6061 gctccttgtg ctctttccag tcgttcagca agaagggcat cgtgtcccat tacatgaaac
     6121 gccacccagg ggtgttccca aagaagcagc acgccagcaa gttggggggc tacttcacgg
     6181 ccgtctatgc agatgagcat gagaagccca cactgatgga agaagaggag agaggcaact
     6241 ttgagaaagc cgaggtggag ggtgaagctc aggaaatcga gtggctccca ttccgctgca
     6301 tcaaatgctt caagctgtcc tttagcactg cagagctgct gtgcatgcat tacactgacc
     6361 accacagtcg ggacctaaag agggacttca tcattctggg caacggcccc cgcttgcaga
     6421 actccaccta ccagtgtaag cactgtgata gcaaactgca aagcacagcc gagctgacct
     6481 cacacttgaa cattcacaat gaggaattcc agaagcgtgc caaacgtcag gagaggagga
     6541 aacagctttt gagcaagcag aaatatgcag atggtgcttt tgcagatttc aaacaagaga
     6601 ggccttttgg tcacttagaa gaggtgccaa agatcaagga gaggaaagtg gtgggctaca
     6661 aatgtaaatt ctgtgtggaa gtgcacccaa cgctccgagc catctgcaat cacctccgaa
     6721 agcacgtcca gtatggcaat gtcccagctg tgtcagctgc tgtgaagggg ctgcgttctc
     6781 atgagaggag ccacctggcc ctggccatgt ttacccgcga ggacaagtac agctgccagt
     6841 attgctcgtt tgtttctgct ttcaggcaca atttggatcg ccatatgcaa acccaccacg
     6901 gacaccataa accattccga tgcaaactct gctccttcaa gtcctcctat aacagccggc
     6961 tgaaaacaca tatactcaaa gctcatgctg gtgagcatgc ctacaagtgt tcttggtgct
     7021 cattctccac catgacaatc agccagctga aggaacactc cctcaaggtc cacggaaaag
     7081 ccctgaccct ccccaggcca cggatcgtca gtctcctctc ctcacactcc caccactcct
     7141 cccaaaaagc taccccggct gaagaagtgg aagactccaa tgactcatca tattcagagc
     7201 ccccagatgt tcagcagcag ttgaaccact atcagtcagc tgccctggca aggaacaaca
     7261 gccgtgttag ccctgtgcct ctttctgggg ctgctgctgg cactgagcag aaaactgaag
     7321 ccgtgcttca ctgcgaattc tgtgaattct cctccggcta catccagagc atcaggcgtc
     7381 attaccggga caagcatggt gggaagaagc ttttcaagtg caaagactgc tccttttaca
     7441 caggctttaa atctgctttt actatgcacg tggaagctgg gcactcagca gttcccgagg
     7501 agggccccaa agatcttcgc tgtcctctct gcctctatca caccaaatac aagcgcaaca
     7561 tgattgacca catcgtgctg caccgagaag agcgtgttgt ccccattgaa gtttgccggt
     7621 ccaaactgtc caaatacttg cagggagtag ttttccgctg tgataagtgt accttcacct
     7681 gctccagtga tgagagcctc cagcaacata tagaaaagca caatgaactg aaaccttaca
     7741 aatgccagct ctgctactat gagaccaagc acacggagga actggacagc caccttcggg
     7801 atgagcataa ggtaagccgt aactttgagc tggttggacg ggttaacttg gatcagctgg
     7861 aacagatgaa ggagaaaatg gagagctcca gcagcgatga tgaggacaag gaagaagaaa
     7921 tgaacagcaa ggctgaagac agagagctga tgagattttc tgaccacggg gctgctctta
     7981 acactgagaa gcgttttcca tgtgaatttt gtggacgggc gttttcacag ggctctgagt
     8041 gggaaagaca tgtgctgaga cacggcatgg cattgaatga caccaagcag gtgagcagag
     8101 aagaaatcca cccaaaagag atcatggaga acagtgttaa aatgccctcc atagaggaaa
     8161 aggaagatga cgaggccatt gggatagact tttccctaaa gaatgaaaca gtagccatct
     8221 gtgtagtaac tgccgacaaa tctctcctgg agaatgcaga ggccaaaaaa gaaggatccg
     8281 actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg
     8341 acaagtagtg agggcccgat atctcgacaa tcaacctctg gattacaaaa tttgtgaaag
     8401 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     8461 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     8521 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     8581 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     8641 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     8701 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     8761 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     8821 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     8881 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     8941 ttgggccgcc tccccgcctg gaaacggggg aggctaactg aaacacggaa ggagacaata
     9001 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg
     9061 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     9121 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     9181 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga
     9241 tctgcgcagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     9301 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     9361 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     9421 aaatcggggc atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     9481 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     9541 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     9601 caaccctatc tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg
     9661 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     9721 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9781 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9841 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     9901 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9961 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
    10021 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
    10081 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
    10141 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
    10201 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
    10261 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
    10321 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
    10381 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
    10441 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
    10501 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
    10561 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
    10621 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
    10681 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
    10741 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
    10801 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
    10861 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
    10921 tcgcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
    10981 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
    11041 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
    11101 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    11161 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
    11221 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    11281 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    11341 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    11401 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    11461 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    11521 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    11581 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    11641 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    11701 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    11761 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    11821 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
    11881 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    11941 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    12001 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    12061 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    12121 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    12181 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    12241 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    12301 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    12361 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    12421 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    12481 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    12541 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    12601 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    12661 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    12721 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    12781 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    12841 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    12901 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    12961 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    13021 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    13081 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    13141 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    13201 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    13261 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    13321 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    13381 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
    13441 tcgactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
    13501 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
    13561 ttgaccgaca attgcatgaa gaatctgctt agg
//
