LOCUS       V041831                 7783 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041831
VERSION     V041831
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2442
                     /label="YTHDF2(NM_016258)"
                     /note="YTHDF2(NM_016258)"
                     /gene="YTHDF2"
     CDS             2449..2475
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2479..2505
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2509..2535
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    2559..3147
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3213..3261
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3463..3891
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3905..4234
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4301..5095
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5271..5392
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5441..5457)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5465..5481
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5489..5519)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5534..5555
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5843..6431)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6602..7462)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7463..7567)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgtc ggccagcagc
      721 ctcttggagc agagaccaaa aggtcaagga aacaaagtac aaaatggatc tgtacatcaa
      781 aaggatggat taaacgatga tgattttgaa ccttacttga gtccacaggc aaggcccaat
      841 aatgcatata ctgccatgtc agattcctac ttacccagtt actacagtcc ctccattggc
      901 ttctcctatt ctttgggtga agctgcttgg tctacggggg gtgacacagc catgccctac
      961 ttaacttctt atggacagct gagcaacgga gagccccact tcctaccaga tgcaatgttt
     1021 gggcaaccag gagccctagg tagcactcca tttcttggtc agcatggttt taatttcttt
     1081 cccagtggga ttgacttctc agcatgggga aataacagtt ctcagggaca gtctactcag
     1141 agctctggat atagtagcaa ttatgcttat gcacctagct ccttaggtgg agccatgatt
     1201 gatggacagt cagcttttgc caatgagacc ctcaataagg ctcctggcat gaatactata
     1261 gaccaaggga tggcagcact gaagttgggt agcacagaag ttgcaagcaa tgttccaaaa
     1321 gttgtaggtt ctgctgttgg tagcgggtcc attactagta acatcgtggc ttccaatagt
     1381 ttgcctccag ccaccattgc tcctccaaaa ccagcatctt gggctgatat tgctagcaag
     1441 cctgcaaaac agcaacctaa actgaagacc aagaatggca ttgcagggtc aagtcttccg
     1501 ccacccccga taaagcataa catggatatt ggaacttggg ataacaaggg tcccgttgca
     1561 aaagccccct cacaggcttt ggttcagaat ataggtcagc caacccaggg gtctcctcag
     1621 cctgtaggtc agcaggctaa caatagccca ccagtggctc aggcatcagt agggcaacag
     1681 acacagccat tgcctccacc tccaccacag cctgcccagc tttcagtcca gcaacaggca
     1741 gctcagccaa cccgctgggt agcacctcgg aaccgtggca gtgggttcgg tcataatggg
     1801 gtggatggta atggagtagg acagtctcag gctggttctg gatctactcc ttcagaaccc
     1861 cacccagtgt tggagaagct tcggtccatt aataactata accccaaaga ttttgactgg
     1921 aatctgaaac atggccgggt tttcatcatt aagagctact ctgaggacga tattcaccgt
     1981 tccattaagt ataatatttg gtgcagcaca gagcatggta acaagagact ggatgctgct
     2041 tatcgttcca tgaacgggaa aggccccgtt tacttacttt tcagtgtcaa cggcagtgga
     2101 cacttctgtg gcgtggcaga aatgaaatct gctgtggact acaacacatg tgcaggtgtg
     2161 tggtcccagg acaaatggaa gggtcgtttt gatgtcaggt ggatttttgt gaaggacgtt
     2221 cccaatagcc aactgcgaca cattcgccta gagaacaacg agaataaacc agtgaccaac
     2281 tctagggaca ctcaggaagt gcctctggaa aaggctaagc aggtgttgaa aattatagcc
     2341 agctacaagc acaccacttc catttttgat gacttctcac actatgagaa acgccaagag
     2401 gaagaagaaa gtgttaaaaa ggaacgtcaa ggtcgtggga aactcgagta cccatacgac
     2461 gtacctgatt atgcaactta tccttatgat gtacctgatt atgctagcta tccttatgat
     2521 gtcccagact acgcatagtg agggcccgat atctcgacaa tcaacctctg gattacaaaa
     2581 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     2641 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     2701 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     2761 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     2821 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     2881 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     2941 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     3001 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     3061 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     3121 ggatctccct ttgggccgcc tccccgcctg gaaacggggg aggctaactg aaacacggaa
     3181 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     3241 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     3301 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     3361 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     3421 ccatagcaga tctgcgcagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     3481 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3541 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3601 gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta cggcacctcg
     3661 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3721 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3781 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttggggattt
     3841 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     3901 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3961 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4021 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4081 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4141 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     4201 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     4261 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     4321 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     4381 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     4441 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     4501 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     4561 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     4621 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     4681 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     4741 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     4801 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     4861 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     4921 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     4981 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     5041 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     5101 actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     5161 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     5221 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     5281 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5341 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5401 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     5461 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     5521 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     5581 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5641 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5701 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5761 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5821 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5881 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5941 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6001 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
     6061 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6121 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6181 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6241 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     6301 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6361 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6421 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6481 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6541 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6601 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6661 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6721 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6781 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6841 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6901 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6961 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7021 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7081 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7141 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7201 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7261 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7321 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7381 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7441 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7501 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7561 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     7621 tcccctatgg tcgactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     7681 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     7741 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agg
//
