LOCUS       V036788                 8175 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036788
VERSION     V036788
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2901
                     /label="XRCC5(NM_021141)"
                     /note="XRCC5(NM_021141)"
                     /gene="XRCC5"
     CDS             2908..2931
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHHHH"
                     /label="8xHis"
     misc_feature    2951..3539
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3605..3653
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3855..4283
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4297..4626
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4693..5487
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5663..5784
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5833..5849)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5857..5873
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5881..5911)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5926..5947
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6235..6823)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6994..7854)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7855..7959)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggt gcggtcgggg
      721 aataaggcag ctgttgtgct gtgtatggac gtgggcttta ccatgagtaa ctccattcct
      781 ggtatagaat ccccatttga acaagcaaag aaggtgataa ccatgtttgt acagcgacag
      841 gtgtttgctg agaacaagga tgagattgct ttagtcctgt ttggtacaga tggcactgac
      901 aatccccttt ctggtgggga tcagtatcag aacatcacag tgcacagaca tctgatgcta
      961 ccagattttg atttgctgga ggacattgaa agcaaaatcc aaccaggttc tcaacaggct
     1021 gacttcctgg atgcactaat cgtgagcatg gatgtgattc aacatgaaac aataggaaag
     1081 aagtttgaga agaggcatat tgaaatattc actgacctca gcagccgatt cagcaaaagt
     1141 cagctggata ttataattca tagcttgaag aaatgtgaca tctccctgca attcttcttg
     1201 cctttctcac ttggcaagga agatggaagt ggggacagag gagatggccc ctttcgctta
     1261 ggtggccatg ggccttcctt tccactaaaa ggaattaccg aacagcaaaa agaaggtctt
     1321 gagatagtga aaatggtgat gatatcttta gaaggtgaag atgggttgga tgaaatttat
     1381 tcattcagtg agagtctgag aaaactgtgc gtcttcaaga aaattgagag gcattccatt
     1441 cactggccct gccgactgac cattggctcc aatttgtcta taaggattgc agcctataaa
     1501 tcgattctac aggagagagt taaaaagact tggacagttg tggatgcaaa aaccctaaaa
     1561 aaagaagata tacaaaaaga aacagtttat tgcttaaatg atgatgatga aactgaagtt
     1621 ttaaaagagg atattattca agggttccgc tatggaagtg atatagttcc tttctctaaa
     1681 gtggatgagg aacaaatgaa atataaatcg gaggggaagt gcttctctgt tttgggattt
     1741 tgtaaatctt ctcaggttca gagaagattc ttcatgggaa atcaagttct aaaggtcttt
     1801 gcagcaagag atgatgaggc agctgcagtt gcactttcct ccctgattca tgctttggat
     1861 gacttagaca tggtggccat agttcgatat gcttatgaca aaagagctaa tcctcaagtc
     1921 ggcgtggctt ttcctcatat caagcataac tatgagtgtt tagtgtatgt gcagctgcct
     1981 ttcatggaag acttgcggca atacatgttt tcatccttga aaaacagtaa gaaatatgct
     2041 cccaccgagg cacagttgaa tgctgttgat gctttgattg actccatgag cttggcaaag
     2101 aaagatgaga agacagacac ccttgaagac ttgtttccaa ccaccaaaat cccaaatcct
     2161 cgatttcaga gattatttca gtgtctgctg cacagagctt tacatccccg ggagcctcta
     2221 cccccaattc agcagcatat ttggaatatg ctgaatcctc ccgctgaggt gacaacaaaa
     2281 agtcagattc ctctctctaa aataaagacc ctttttcctc tgattgaagc caagaaaaag
     2341 gatcaagtga ctgctcagga aattttccaa gacaaccatg aagatggacc tacagctaaa
     2401 aaattaaaga ctgagcaagg gggagcccac ttcagcgtct ccagtctggc tgaaggcagt
     2461 gtcacctctg ttggaagtgt gaatcctgct gaaaacttcc gtgttctagt gaaacagaag
     2521 aaggccagct ttgaggaagc gagtaaccag ctcataaatc acatcgaaca gtttttggat
     2581 actaatgaaa caccgtattt tatgaagagc atagactgca tccgagcctt ccgggaagaa
     2641 gccattaagt tttcagaaga gcagcgcttt aacaacttcc tgaaagccct tcaagagaaa
     2701 gtggaaatta aacaattaaa tcatttctgg gaaattgttg tccaggatgg aattactctg
     2761 atcaccaaag aggaagcctc tggaagttct gtcacagctg aggaagccaa aaagtttctg
     2821 gcccccaaag acaaaccaag tggagacaca gcagctgtat ttgaagaagg tggtgatgtg
     2881 gacgatttat tggacatgat actcgagcac catcaccatc accatcacca ttagggcccg
     2941 atatctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     3001 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     3061 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     3121 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     3181 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     3241 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     3301 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     3361 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     3421 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     3481 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     3541 tggaaacggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     3601 atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg
     3661 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     3721 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     3781 gctcgcagcc aacgtcgggg cggcaggccc tgccatagca gatctgcgca gctggggctc
     3841 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3901 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3961 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt
     4021 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4081 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4141 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4201 ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat
     4261 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     4321 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4381 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4441 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4501 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4561 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4621 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     4681 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     4741 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     4801 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     4861 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     4921 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     4981 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     5041 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     5101 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     5161 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     5221 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     5281 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     5341 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     5401 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     5461 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa tgaccgacca
     5521 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     5581 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     5641 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     5701 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5761 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     5821 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5881 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5941 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6001 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6061 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6121 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6181 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7981 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc
     8041 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     8101 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     8161 aagaatctgc ttagg
//
