LOCUS       V047412                 8932 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047412
VERSION     V047412
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..3591
                     /label="XPOT(NM_007235)"
                     /note="XPOT(NM_007235)"
                     /gene="XPOT"
     CDS             3598..3624
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3628..3654
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3658..3684
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    3708..4296
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4362..4410
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4612..5040
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5054..5383
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5450..6244
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6420..6541
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6590..6606)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6614..6630
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6638..6668)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6683..6704
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6992..7580)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7751..8611)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8612..8716)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgga tgaacaggct
      721 ctattagggc taaatccaaa tgctgattca gactttagac aaagggccct ggcctatttt
      781 gagcagttaa aaatttcccc agatgcctgg caggtgtgtg cagaagctct agcccagagg
      841 acatacagtg atgatcatgt gaagtttttc tgctttcaag tactggaaca tcaagttaaa
      901 tacaaatact cagaactaac cactgttcaa caacagctaa ttagggagac gctcatatca
      961 tggctgcaag ctcagatgct gaatccccaa ccagagaaga cctttatacg aaataaagcc
     1021 gcccaagtct tcgccttgct ttttgttaca gagtatctca ctaagtggcc caagtttttt
     1081 tttgacattc tctcagtagt ggacctaaat ccaaggggag tagatctcta cctgcgaatc
     1141 ctcatggcta ttgattcaga gttggtggat cgtgatgtgg tgcatacatc agaggaggct
     1201 cgtaggaata ctctcataaa agataccatg agggaacagt gcattccaaa tctggtggaa
     1261 tcatggtacc aaatattaca aaattatcag tttactaatt ctgaagtgac gtgtcagtgc
     1321 cttgaagtag ttggggctta tgtctcttgg atagacttat cccttatagc caatgatagg
     1381 tttataaata tgctgctagg tcatatgtca atagaagttc tacgggaaga agcatgtgac
     1441 tgtttatttg aagttgtaaa taaaggaatg gaccctgttg ataaaatgaa actagtggaa
     1501 tctttgtgtc aagtattaca gtctgctggg tttttcagca ttgaccagga agaagatgtt
     1561 gacttcctgg ccagattttc taagttggta aatggaatgg gacagtcatt gatagttagt
     1621 tggagtaaat taattaagaa tggggatatt aagaatgctc aagaggcact acaagctatt
     1681 gaaacaaaag tggcactgat gttgcagcta ctaattcatg aggatgatga tatttcttct
     1741 aatattattg gattttgtta cgattatctt catattttga aacagcttac agtgctctcg
     1801 gatcagcaaa aagctaatgt agaggcaatc atgttggccg ttatgaaaaa attgacttac
     1861 gatgaagaat ataactttga aaatgagggt gaagatgaag ccatgtttgt agaatataga
     1921 aaacaactga agttactgtt ggacaggctt gctcaagttt caccagagtt actactggcc
     1981 tctgttcgca gagtttttag ttctacactg cagaattggc agactacacg gtttatggaa
     2041 gttgaagtag caataagatt gctgtatatg ttggcagaag ctcttccagt atctcatggt
     2101 gctcacttct caggtgatgt ttcaaaagct agtgctttgc aggatatgat gcgaactctg
     2161 gtaacatcag gagtcagttc ctatcagcat acatctgtga cattggagtt cttcgaaact
     2221 gttgttagat atgaaaagtt tttcacagtt gaacctcagc acattccatg tgtactaatg
     2281 gctttcttag atcacagagg tctgcggcat tccagtgcaa aagttcggag caggacggct
     2341 tacctgtttt ctagatttgt caaatctctc aataagcaaa tgaatccttt cattgaggat
     2401 attttgaata gaatacaaga tttattagag ctttctccac ctgagaatgg ccaccagtcc
     2461 ttactgagca gcgatgatca actttttatt tatgagacag ctggagtgct gattgttaat
     2521 agtgaatatc cggcagaaag gaaacaagcc ttaatgagga atctgttgac tccactaatg
     2581 gagaagttta aaattctgtt agaaaagttg atgctggcac aagatgaaga aaggcaagcc
     2641 tctctagcag actgtcttaa ccatgctgtt ggatttgcaa gtcgaaccag taaagctttc
     2701 agcaacaaac agactgtgaa acaatgtggc tgttccgaag tttatctgga ctgtttacag
     2761 acattcttgc cagccctcag ttgtccctta caaaaggata ttctcagaag tggagtccgt
     2821 actttccttc atcgaatgat tatttgcctg gaggaagaag ttcttccgtt cattccatct
     2881 gcttcagaac atatgctcaa agattgtgaa gcaaaagatc tccaggagtt cattcctctt
     2941 atcaaccaga ttacggccaa attcaagata caggtatccc cgtttttaca acagatgttc
     3001 atgcccctgc ttcatgcaat ttttgaagtg ctgctccggc cagcagaaga aaatgaccag
     3061 tctgctgctt tagagaagca gatgttgcgg aggagttact ttgctttcct gcaaacagtc
     3121 acaggcagtg ggatgagcga agttatagca aatcaaggtg cagagaatgt agaaagagtg
     3181 ttggttactg ttatccaagg agcagttgaa tatccagatc caattgcaca gaaaacatgt
     3241 tttatcatcc tctcaaagtt ggtagaactc tggggaggta aagatggacc agtgggattt
     3301 gctgattttg tttataagca cattgtcccc gcatgtttcc tagcaccttt aaaacaaacc
     3361 tttgacctgg cagatgcaca aacagtattg gctttatctg agtgtgcagt gacactgaaa
     3421 acaattcatc tcaaacgggg cccagaatgt gttcagtatc ttcaacaaga atacctgccc
     3481 tccttgcaag tagctccaga aataattcag gagttttgtc aagcgcttca gcagcctgat
     3541 gctaaagttt ttaaaaatta cttaaaggtg ttcttccaga gagcaaagcc cctcgagtac
     3601 ccatacgacg tacctgatta tgcaacttat ccttatgatg tacctgatta tgctagctat
     3661 ccttatgatg tcccagacta cgcatagtga gggcccgata tctcgacaat caacctctgg
     3721 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     3781 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     3841 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     3901 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     3961 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     4021 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     4081 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     4141 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     4201 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     4261 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aaacggggga ggctaactga
     4321 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     4381 taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     4441 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     4501 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     4561 caggccctgc catagcagat ctgcgcagct ggggctctag ggggtatccc cacgcgccct
     4621 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4681 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4741 gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac
     4801 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4861 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4921 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4981 tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5041 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     5101 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     5161 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     5221 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5281 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     5341 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     5401 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     5461 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     5521 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     5581 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     5641 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     5701 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     5761 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     5821 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     5881 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     5941 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     6001 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     6061 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     6121 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     6181 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     6241 ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     6301 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     6361 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     6421 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6481 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6541 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     6601 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6661 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6721 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6781 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6841 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6901 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6961 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7021 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7081 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7141 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     7201 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7261 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7321 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7381 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     7441 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7501 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7561 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7621 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7681 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7741 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7801 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7861 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7921 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7981 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8041 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8101 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8161 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8221 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8281 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8341 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8401 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8461 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8521 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8581 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8641 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8701 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     8761 atctcccgat cccctatggt cgactctcag tacaatctgc tctgatgccg catagttaag
     8821 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     8881 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gg
//
