LOCUS V018781 6983 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018781 VERSION V018781 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 665..1663 /label="XCR1(NM_005283)" /note="XCR1(NM_005283)" /gene="XCR1" CDS 1670..1735 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 1759..2347 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2413..2461 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 2663..3091 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3105..3434 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3501..4295 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4471..4592 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4641..4657) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4665..4681 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4689..4719) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4734..4755 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5043..5631) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5802..6662) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6663..6767) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgc 661 caccatggag tcctcaggca acccagagag caccaccttt ttttactatg accttcagag 721 ccagccgtgt gagaaccagg cctgggtctt tgctaccctc gccaccactg tcctatactg 781 cctggtgttt ctcctcagcc tagtgggcaa cagcctggtc ctgtgggtcc tggtgaagta 841 tgagagcctg gagtccctca ccaacatctt catcctcaac ctgtgcctct cagacctggt 901 gttcgcctgc ttgttgcctg tgtggatctc cccataccac tggggctggg tgctgggaga 961 cttcctctgc aaactcctca atatgatctt ctccatcagc ctctacagca gcatcttctt 1021 cctgaccatc atgaccatcc accgctacct gtcggtagtg agccccctct ccaccctgcg 1081 cgtccccacc ctccgctgcc gggtgctggt gaccatggct gtgtgggtag ccagcatcct 1141 gtcctccatc ctcgacacca tcttccacaa ggtgctttct tcgggctgtg attattccga 1201 actcacgtgg tacctcacct ccgtctacca gcacaacctc ttcttcctgc tgtccctggg 1261 gattatcctg ttctgctacg tggagatcct caggaccctg ttccgctcac gctccaagcg 1321 gcgccaccgc acggtcaagc tcatcttcgc catcgtggtg gcctacttcc tcagctgggg 1381 tccctacaac ttcaccctgt ttctgcagac gctgtttcgg acccagatca tccggagctg 1441 cgaggccaaa cagcagctag aatacgccct gctcatctgc cgcaacctcg ccttctccca 1501 ctgctgcttt aacccggtgc tctatgtctt cgtgggggtc aagttccgca cacacctgaa 1561 acatgttctc cggcagttct ggttctgccg gctgcaggca cccagcccag cctcgatccc 1621 ccactcccct ggtgccttcg cctatgaggg cgcctccttc tacctcgagg actacaaaga 1681 ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg acaagtagtg 1741 agggcccgat atctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 1801 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 1861 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 1921 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 1981 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 2041 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 2101 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg 2161 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 2221 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 2281 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 2341 tccccgcctg gaaacggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa 2401 cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt 2461 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat 2521 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa 2581 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga tctgcgcagc 2641 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 2701 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 2761 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc 2821 atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 2881 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 2941 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 3001 tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat 3061 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt 3121 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 3181 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 3241 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 3301 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 3361 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 3421 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga 3481 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg 3541 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 3601 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 3661 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg 3721 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat 3781 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat 3841 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 3901 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 3961 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 4021 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 4081 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 4141 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 4201 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 4261 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgcgaaatg 4321 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 4381 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 4441 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 4501 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 4561 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc 4621 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 4681 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 4741 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 4801 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 4861 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 4921 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 4981 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 5041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 5101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 5161 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 5221 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 5281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 5341 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 5401 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 5461 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 5521 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 5581 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 5641 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 5701 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 5761 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 5821 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 5881 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 5941 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 6001 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 6061 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 6121 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 6181 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 6241 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 6301 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 6361 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 6421 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 6481 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 6541 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 6601 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 6661 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 6721 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 6781 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tcgactctca 6841 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg 6901 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca 6961 attgcatgaa gaatctgctt agg //