LOCUS       V031309                 7416 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031309
VERSION     V031309
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3563
                     /label="Vav1(NM_011691)"
                     /note="Vav1(NM_011691)"
                     /gene="Vav1"
     CDS             3582..3611
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3630..3653
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3673..3689)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3700..4322
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4471..5059)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5388..5435
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5667..6461)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6496..6853)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6855..6959)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6986..7414
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagctctgg cgacagtgca cccactggct gatccagtgt cgggtgctgc
     1081 ctcccagcca ccgtgtgacc tgggaggggg cccaggtgtg tgagctggca caggcactgc
     1141 gggacggtgt cctcttgtgc caattgctta acaacctgct tccccaggcc attaatcttc
     1201 gcgaggttaa cttgcggccc cagatgtccc agttcctttg tcttaagaac attcgaacct
     1261 tcctgtctac ttgctgtgag aagttcggcc tcaagcgcag tgaactcttt gaggcttttg
     1321 acctcttcga tgtgcaggac tttggaaagg tcatctacac cctgtctgct ctgtcatgga
     1381 cacccattgc ccagaacaaa ggaatcatgc ccttcccaac agaggacagc gctctgaacg
     1441 acgaagatat ttacagtggc ctttcagacc agattgatga caccgcagag gaagacgagg
     1501 acctttatga ctgcgtggaa aatgaggagg cagaggggga cgagatctac gaggacctaa
     1561 tgcgcttgga gtcggtgcct acgccaccca agatgacaga gtatgataag cgctgctgct
     1621 gcctgcggga gatccagcag acggaggaga agtatacaga cacactgggc tccatccagc
     1681 agcacttcat gaagcctctg cagcgattcc ttaagcctca agacatggag accatctttg
     1741 tcaacattga ggagctgttc tctgtgcata cccacttctt aaaggaactg aaggatgccc
     1801 tggctggccc gggagcaaca acactgtatc aggtcttcat caagtacaag gagaggttcc
     1861 tggtttatgg ccgttattgc agtcaggtgg agtcagccag caagcacttg gatcaagtgg
     1921 ccacagcacg ggaggatgtg cagatgaagc tggaggaatg ttctcaaaga gctaacaatg
     1981 gccgattcac cctacgggat ctgctgatgg tacctatgca gcgggtgctg aagtaccacc
     2041 tccttctcca ggagctagtg aaacacacac aggatgctac agagaaggag aacctgcggt
     2101 tggccctgga cgccatgagg gacctggcac agtgcgtgaa cgaggtcaag agggacaatg
     2161 aaaccctacg gcagatcaca aactttcagc tgtccattga gaacctggac cagtctctgg
     2221 ctaactatgg ccggcccaag attgacggtg agctcaagat tacctcagtg gagcgtcgct
     2281 caaagacaga caggtatgcc ttcctgctgg acaaagcact gctcatctgt aaacgccgcg
     2341 gggactctta cgacctcaaa gcctcggtga acttgcacag cttccaagtt cgagatgact
     2401 cctccgggga gcgagacaac aagaagtgga gccatatgtt ccttctgatt gaggatcaag
     2461 gcgcccaggg ctatgagctg ttcttcaaga ctcgggagct gaagaagaag tggatggaac
     2521 agttcgaaat ggccatctcc aacatttacc cagagaatgc tacagccaat gggcatgatt
     2581 ttcagatgtt ctcctttgag gagaccactt cctgcaaggc ctgccagatg ttactcagag
     2641 gcacattcta ccagggatat cgctgttaca ggtgccgggc acctgcacac aaggagtgtc
     2701 tggggagagt gcctccctgt ggtcgccatg ggcaagattt cgcaggaacc atgaagaagg
     2761 acaagctcca tcgaagggcc caggacaaga aaaggaatga attgggtctg cctaagatgg
     2821 aagtgtttca ggaatactat gggatccctc ctccacctgg agcctttggg ccatttttac
     2881 ggctcaaccc tggggacatt gtggagctca ctaaggcaga ggctgagcac aactggtggg
     2941 agggaaggaa tactgctaca aatgaagtcg gctggtttcc ctgtaacaga gtgcatccct
     3001 atgtccacgg ccctcctcag gacctgtctg tgcatctctg gtatgcgggc cctatggaac
     3061 gagcaggcgc tgagggcatc ctcaccaacc gttctgatgg gacctatctg gtgcggcaga
     3121 gggtgaaaga tacagcggag ttcgccatca gcattaagta taacgtggag gtcaagcata
     3181 ttaaaatcat gacgtcagag gggttgtacc ggatcacaga gaagaaggct ttccggggcc
     3241 ttctggaact ggtagagttt tatcagcaga attccctcaa agattgcttc aagtcgttgg
     3301 acaccacctt gcagtttcct tataaggaac ctgagaggag agccatcagc aagccaccag
     3361 ctggaagcac caagtatttt ggcactgcca aagcccgcta cgacttctgt gcccgggaca
     3421 ggtcggaact gtcccttaag gagggtgata tcatcaagat cctcaataag aagggacagc
     3481 aaggctggtg gcgtggggag atctacggcc ggatcggctg gttcccttct aactatgtgg
     3541 aggaagacta ttccgaatat tgcacgcgta cgcggccgct cgagcagaaa ctcatctcag
     3601 aagaggatct ggcagcaaat gatatcctgg attacaagga tgacgacgat aaggtttaaa
     3661 cggccggccg cggtcatagc tgtttcctga acagatcccg ggtggcatcc ctgtgacccc
     3721 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     3781 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     3841 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     3901 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     3961 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     4021 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     4081 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     4141 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttaaaata actataccag
     4201 caggaggacg tccagacaca gcataggcta cctggccatg cccaaccggt gggacatttg
     4261 agttgcttgc ttggcactgt cctctcatgc gttgggtcca ctcagtagat gcctgttgaa
     4321 ttgggtacgc ggccagcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     4381 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4441 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     4501 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     4561 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     4621 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4681 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4741 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4801 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4861 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     4921 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4981 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5041 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5101 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5161 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga
     5221 ggctatggca gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact
     5281 tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg
     5341 tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc
     5401 aacaccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat
     5461 tgtctccttc cgtgtttcag ttagcctccc cctagggtgg gcgaagaact ccagcatgag
     5521 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     5581 ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
     5641 ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
     5701 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     5761 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcgatccgcc
     5821 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     5881 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg
     5941 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     6001 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     6061 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     6121 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     6181 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     6241 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg
     6301 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     6361 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     6421 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
     6481 gtctcttgat cgatctttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     6541 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     6601 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     6661 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     6721 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     6781 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt
     6841 ctttccgcct caggactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6901 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6961 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7021 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7081 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7141 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7201 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7261 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7321 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7381 aatgagctga tttaacaaaa atttaacgcg aatttt
//
