LOCUS       V031005                 6195 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031005
VERSION     V031005
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2342
                     /label="Usp27x(NM_019461)"
                     /note="Usp27x(NM_019461)"
                     /gene="Usp27x"
     CDS             2361..2390
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2409..2432
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2452..2468)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2479..3101
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3250..3838)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4167..4214
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4446..5240)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5275..5632)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5634..5738)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5765..6193
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gtgtaaagac tatgtgtatg acatagacat tgagcaaatt gccaaagaag
     1081 agcaaggaga agccttgaaa ttacaagcgt ccacctcgac agaggtttct cagcaacagt
     1141 gttcagtgcc gggacttggg gagaaatatc ccacctggga aacgaccaaa cctgaactag
     1201 aacttctggg gcacaaccca cggagaagaa ggatcgcctc cagctttacc atcggcttac
     1261 gaggactaat taatcttggc aacacgtgtt ttatgaactg cattgtccag gccttgaccc
     1321 acactccaat actgagagat ttctttctct ctgacaggca ccggtgtgaa atgcccagcc
     1381 ctgagttgtg tctggtctgt gagatgtcgt cgctctttcg ggagctgtat tctggaaacc
     1441 catctcctca tgtgccctat aagttactgc acctggtgtg gatacacgct cgtcatctag
     1501 cagggtacag gcagcaggat gcccacgagt tcctcatcgc tgcgctagat gtcctgcata
     1561 ggcactgcaa aggtgacgat gttggcaagg tggccagcaa ccccaaccac tgtaactgca
     1621 tcatagacca aatcttcaca ggtggcctgc agtccgatgt cacctgtcaa gcctgccatg
     1681 gtgtctccac cactatagac ccatgctggg acattagtct ggacttgcct ggctcttgca
     1741 catccttctg gcccatgagc cctgggaggg agagcagttt gaatggcgaa agccacatcc
     1801 caggcatcac cactctcacg gactgcttgc gaaggtttac aaggccagag cacttaggaa
     1861 gtagtgccaa aatcaagtgt ggtagttgcc aaagctacca agaatctacc aaacagctca
     1921 ctatgaagaa gttaccagta gttgcctgct ttcatttcaa acggtttgaa cactcagcga
     1981 aacagaggcg caagatcact acgtacattt cctttcctct ggagctggat atgacaccat
     2041 ttatggcgtc aagtaaagag acccgggtga atggacagtt gcagctccca accaatagtg
     2101 cgaacaacga gaataagtat tccttgtttg ctgtggttaa tcaccaagga accttggaga
     2161 gtggccacta caccagcttc attcggcacc acagggacca gtggttcaag tgtgatgatg
     2221 ccgtaatcac caaggccagt attaaggatg tgctggacag tgaagggtat ttactgttct
     2281 atcacaaaca ggtcctggaa cctgagccag aaaaggtgaa agaaatgact ccacaagcct
     2341 acacgcgtac gcggccgctc gagcagaaac tcatctcaga agaggatctg gcagcaaatg
     2401 atatcctgga ttacaaggat gacgacgata aggtttaaac ggccggccgc ggtcatagct
     2461 gtttcctgaa cagatcccgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc
     2521 ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt
     2581 ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag
     2641 gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt
     2701 gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg
     2761 cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg
     2821 tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct
     2881 caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct
     2941 cccttccctg tccttctgat tttaaaataa ctataccagc aggaggacgt ccagacacag
     3001 cataggctac ctggccatgc ccaaccggtg ggacatttga gttgcttgct tggcactgtc
     3061 ctctcatgcg ttgggtccac tcagtagatg cctgttgaat tgggtacgcg gccagcggcg
     3121 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3181 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3241 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3301 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3361 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3421 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3481 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3541 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3601 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3661 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     3721 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3781 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3841 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3901 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3961 aagttttaaa tcaatctaaa gtatatatga gtaacctgag gctatggcag ggcctgccgc
     4021 cccgacgttg gctgcgagcc ctgggccttc acccgaactt ggggggtggg gtggggaaaa
     4081 ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt ggggtatcga cagagtgcca
     4141 gccctgggac cgaaccccgc gtttatgaac aaacgaccca acaccgtgcg ttttattctg
     4201 tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt
     4261 tagcctcccc ctagggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat
     4321 ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga
     4381 atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga accccagagt
     4441 cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg
     4501 gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata
     4561 tcacgggtag ccaacgctat gtcctgatag cgatccgcca cacccagccg gccacagtcg
     4621 atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg
     4681 gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga gcctggcgaa cagttcggct
     4741 ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc
     4801 cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga
     4861 tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca
     4921 aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc
     4981 gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat
     5041 agccgcgctg cctcgtcttg cagttcattc agggcaccgg acaggtcggt cttgacaaaa
     5101 agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc
     5161 tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc
     5221 aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc gatctttgca
     5281 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
     5341 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     5401 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     5461 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct
     5521 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     5581 ctttccacac cctaactgac acacattcca cagctggttc tttccgcctc aggactcttc
     5641 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     5701 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     5761 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5821 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5881 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5941 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6001 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6061 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6121 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6181 tttaacgcga atttt
//
