LOCUS V040982 6604 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V040982 VERSION V040982 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..1260 /label="Ube2f(NM_026454)" /note="Ube2f(NM_026454)" /gene="Ube2f" CDS 1267..1356 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" misc_feature 1380..1968 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2034..2082 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 2284..2712 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2726..3055 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3122..3916 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4092..4213 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4262..4278) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4286..4302 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4310..4340) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4355..4376 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4664..5252) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5423..6283) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6284..6388) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgct aacgctggca 721 agcaagttga agcgggatga tggtctcaaa ggatcccgga catcagcctc cacatctgac 781 tctacccgga gggtttctgt gagagacaag ttgcttgtta aagaggttgc agaacttgaa 841 gctaatttac cttgtacatg taaagtacat tttcctgatc caaacaagct tcattgcttt 901 cagctgactg taagcccaga tgagggttac taccagggtg gaaaatttca gtttgaaact 961 gaagttcccg atgcctacaa catggtgcct cccaaggtga aatgcttgac taaaatctgg 1021 caccccaaca tcacagaaac gggggaaata tgtctaagtt tactaagaga acattcaatt 1081 gatggcactg gctgggctcc cactagaaca ttaaaggatg ttgtttgggg attaaactct 1141 ttatttaccg atctcttgaa ttttgatgat ccactgaata ttgaagctgc agaacatcat 1201 ttgcgggaca aggaggattt tcgggacaaa gtggatgaat acatcaaacg ctatgccaga 1261 ctcgaggaac aaaaactaat atcagaggaa gatttggaac agaaactaat cagtgaagaa 1321 gatttagagc aaaagttaat ttccgaggag gacttatagt gagggcccga tatctcgaca 1381 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 1441 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 1501 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 1561 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 1621 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 1681 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 1741 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg 1801 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 1861 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 1921 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaaacgggg 1981 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat 2041 aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt 2101 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg 2161 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca 2221 acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct agggggtatc 2281 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 2341 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 2401 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat 2461 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 2521 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 2581 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 2641 tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 2701 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc 2761 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 2821 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 2881 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 2941 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 3001 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 3061 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg 3121 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 3181 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 3241 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 3301 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 3361 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 3421 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 3481 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 3541 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 3601 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 3661 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 3721 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 3781 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 3841 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 3901 tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca 3961 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 4021 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 4081 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 4141 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 4201 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat 4261 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 4321 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 4381 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 4441 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 4501 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 4561 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 4621 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 4681 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 4741 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 4801 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat 4861 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 4921 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 4981 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 5041 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 5101 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 5161 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 5221 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 5281 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 5341 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 5401 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 5461 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 5521 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 5581 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 5641 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 5701 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 5761 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 5821 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 5881 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 5941 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 6001 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 6061 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 6121 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 6181 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 6241 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 6301 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 6361 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg 6421 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc 6481 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc 6541 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct 6601 tagg //