LOCUS       V041692                11982 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041692
VERSION     V041692
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..6662
                     /label="Trp53bp1(NM_013735)"
                     /note="Trp53bp1(NM_013735)"
                     /gene="Trp53bp1"
     CDS             6669..6734
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    6758..7346
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7412..7460
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7662..8090
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8104..8433
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8500..9294
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9470..9591
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9640..9656)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9664..9680
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9688..9718)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9733..9754
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10042..10630)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10801..11661)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11662..11766)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgcc aggggagcag atggacccta
      781 ctggaagtca attggattca gatttctctc agcaagacac tccttgcctg ataatagaag
      841 attctcagcc tgaaagccag gttctggaag aagatgcagg ctctcacttc agcgtgctat
      901 ctcgacacct tcctaatctg cagatgcaca aagagaaccc cgtgttggat attgtatcaa
      961 atccggaaca atctgctgta gaacaaggag acagtaatag ctcattcaat gaacatctga
     1021 aagaaaagaa agcttcagat cctgtggagt cttctcattt gggtaccagt ggttccatca
     1081 gtcaggtcat tgaacggtta cctcagccaa acaggacaag cagtgctctg gcagtgacag
     1141 tagaagctgc ttctctccca gaggaggaga aggaagaaga ggagttagag gaggagaagg
     1201 aaggggtggg agcaaacgct cccggtgctg actcccttgc tgctgaagat tctgcttcat
     1261 cacagttggg ctttggagtt ctggaactgt cccagagcca ggatgttgaa gaacatacag
     1321 tgccatatga tgtcaaccaa gagcatctgc agttggtgac cactaactcg ggttctagcc
     1381 cgctatctga tgtggatgcg agcactgcaa ttaaatgtga agaacagccc actgaagata
     1441 ttgccatgat agaacagccc agcaaagaca tccctgttac agtacagccc ggtaaaggta
     1501 tccatgtggt agaagaacaa aatctaccac ttgtaaggtc cgaagaccgg ccgtccagtc
     1561 ctcaagtttc tgttgctgct gtggaaacaa aggaacaggt acctgcccgg gagctgctgg
     1621 aagaggggcc gcaggttcag ccgtcatcag agcctgaggt ttcctcaacc caggaggact
     1681 tgtttgacca gagtagtaaa acagcttctg atggttgttc tactccttca agggaggaag
     1741 gtgggtgctc tccggtttcc acacctgcta ccaccctgca gctcctgcag ctctctggtc
     1801 agaagcccct tgttcaggag agtctttcca cgaattcctc agatcttgtt gctccttccc
     1861 ctgatgcttt ccgatctacc ccttttatcg ttcctagcag tcccacagag caaggaggga
     1921 gaaaagatga gcccatggat atgtcagtga tacctgtagg aggggagcct ttccagaagc
     1981 ttcatgatga cgaagcaatg gagacagaaa aaccccttct cccgtctcag cctgctgtgt
     2041 caccgcaagc atcaacacca gtgtctcgga gcacgccagt tttcactcct ggctctcttc
     2101 ccatcccgtc ccagcccgag ttctctcatg acattttcat tccatcacca agtctggaag
     2161 aaccatcaga tgatgtgaag aaaggtggag gtttacatag ctcatctctt actgttgagt
     2221 gttccaagac ttcagagagt gaaccaaaga atttcactga tgaccttggg ctctccatga
     2281 caggggattc ttgcaaactg atgctttcta caagtgagta tagtcagtcc tcaaagatgg
     2341 agagcttggg ttctcccagg actgaggaag acagagagaa cacacagatt gacgatactg
     2401 aacctttgtc tccagttagc aattctaaac ttcctgctga cagtgagaat gtcctggtga
     2461 ctccatcgca ggacgaccag gtagaaatga gtcagaatgt agataaagca aaagaggatg
     2521 aaacggagga cagaggtgac tgtaaaggca gagaagacgc ggttgctgaa gatgtttgca
     2581 tcgacctcac ttgtgattct gggagtcagg cagttccttc tccagctacc cgttcggagg
     2641 cactttccag tgtcttagat caggaggaag ctatggacac taaagaacac catccagagg
     2701 aagggttttc gggatctgaa gtagaagaag tccctgagac tccctgtgga agtcacagag
     2761 aggagcccaa agaagaaccg atggaaagta tcccactgca cctttctctg actgaaaccc
     2821 agtctgaggc attgtgtctg cagaaggaag cccccaaaga ggaatgccca gaagctatgg
     2881 aagttgaaac cagtgtgatt agtattgact ccccccagaa actgcaagta cttgaccagg
     2941 aattagagca taaggaccca gacacctggg aagaagctac ttctgaggac tccagtgttg
     3001 ttatcgttga tgtgaaggag ccctctcctc gagctgatgt ttcctgtgaa cctttggagg
     3061 aggtggagaa atgctctgac tcccagtcct gggagggtgt ggctccagag gaagaaccat
     3121 gtgctgagaa tagattagat actccagaag aaaagcgtat agaatgtgat ggagattcaa
     3181 aagcagagac cacagaaaag gacgctgtaa cagaagactc tccacaacct cctttgcctt
     3241 cagtgagaga cgaacctgtc agacccgatc aggagacaca gcagccccaa gttcaagaga
     3301 aagagagccc agtgactgta gatgcagaag tggctgatga caagcagctg ggaccagagg
     3361 gcgcatgcca gcagcttgag aaggcccctg cctgtgcctc acagagtttc tgtgaaagtt
     3421 ctagtgaaac tccatttcat ttcactttgc ctaaagaagg tgatattatc ccaccattga
     3481 ctggcgcaac cccacctctt attgggcacc taaaattgga gcccaagaga catagtactc
     3541 ctattgggat tagcaactat ccagaaagca ccatagcaac cagtgatgtc acatctgaaa
     3601 gcatggtgga gatcaatgat cctctacttg ggaatgaaaa aggggattct gagtctgccc
     3661 cagaaatgga tggaaaactg tctctgaaaa tgaaactggt tagtcctgag acagaggcca
     3721 gtgaagaatc tttgcaattt agcctggaaa agcctacaac tgctgagaga aaaaatggat
     3781 ctactgccat tgcagagcct gttgccagtc tccagaagcc ggtgcctgtg tttggctgca
     3841 tctatgaagc ccagcaagag aaggaggctc aaagtgaggc tcctccctct gcacccgaca
     3901 gggcgaattt gctccatttt ccaagtgctc aagaagaaga caaagaaaga ccagatgtta
     3961 ccccaaaact caggcagagt gaacagcctg tgaagcctgt tgggcctgtc atggacgatg
     4021 ctgctcctga ggactcagct tctcctgttt cgcagcagag ggcctcacag gagcagaggg
     4081 cctcacagga gcccttcagt cctgcagagg acgtgatgga aacagacctg ctggaaggac
     4141 tggctgctaa ccaggacaga cctagtaaga tgttgatgga caggcccacc cagagtaaca
     4201 tcgggatcca gaccgtggac cattccctgt gcgccccaga aactgtctcg gcagcaactc
     4261 agactgtgaa gagtgtgtgt gagcagggga ccagtacagc ggagcagaac tctgggaaac
     4321 aagatgccac tgtgcagact gagaggggga gtggagagaa acctgccagt gctcccgtgg
     4381 acgacacaga gtccctccac agccaggggg aagaggaatt tgaaatgccc cagcctccgc
     4441 atggccatgt cttgcatcgc cacatgagaa ccattcgaga agtccgtaca ctcgtcaccc
     4501 gcgtcatcac agatgtttat tatgtggatg ggacagaagt ggaaaggaaa gtaacggagg
     4561 agactgaaga accaatcgta gagtgtcagg aatgtgaaac agaagtttcc ccttcacaga
     4621 ctggaggttc ctctggggac ctgggggaca tcagctcctt ctcctccaaa gcttccagct
     4681 cacaccacac atccagtgga acaagtctct cagccataca cagcagtggc agctcagggc
     4741 gaggagctgg gccactcaaa ggaaaagcca gtgggacaga agctgcagat tttgccttac
     4801 ccagttcccg aggaggccca ggaaaactga gtcctagaaa agggatcagt cagacagggg
     4861 caccggtgtg tgaggaagat ggtgatgcag gccttggcat cagacaggga gggaaggctc
     4921 cagttacacc tcgtgggcgt gggcgaaggg gccgcccacc ttctcggacc actggaacca
     4981 gagaaacagt tgtctctggt ccgttgggcg tagaagatat ttcacctagc atgtcaccag
     5041 atgacaagtc cttcacccgc attatgcctc gtgtgccaga ttctaccaaa cggacggatg
     5101 ccagttctag tactttgcgg cggagtgatt ctccagagat tccttttcag gctgctactg
     5161 gttcctctga tggcttggat tcctcatctt caggaaacag ctttgtgggt ctccgtgttg
     5221 tagctaagtg gtcatccaat ggctactttt actctgggaa gatcacccga gatgtggggg
     5281 ctgggaagta caagctgctc tttgatgatg ggtacgaatg tgacgtgctg ggcaaagaca
     5341 ttctcctgtg tgaccccatc cccctggaca ctgaagtgac agccctctca gaagatgaat
     5401 atttcagtgc aggagtggtg aaaggacata ggaaggagtc tggggagctg tattacagca
     5461 ttgaaaaaga aggccaaagg aagtggtata aacgaatggc agtcattctg tccttggagc
     5521 aaggaaacag actgagagag caatatgggc ttggcccata tgaagctgtc acaccgctca
     5581 cgaaggcagc agatatcagc ttagacaatt tggtggaagg aaagcggaaa cggcgcagta
     5641 acatcagctc cccagtcacc cccactgccg ccagtagcag cagcacaacg cccacacgta
     5701 aagccacgga gagtccccgt gcttccacgg gagtcccatc aggcaaaagg aaacttccga
     5761 cttctgaaga ggaacgctct cctgctaagc gaggtcgaaa gtctgccacc gtgaaacctg
     5821 gaacagtggg ggcagcagag tttgtgagtc cctgtgagac tggagacaac ataggtgagc
     5881 cttctgtcct agaagagcca agagggcctt tgcccctcaa caagaccttg tttctgggct
     5941 atgcctttct cctcaccatg gctacaacta gtgacaagct ggccagtcgc tctaagctgc
     6001 tagatggtcc tacaggaagc agtgaagaag aggaggaatt tttagaaatt cctcctttca
     6061 acaagcagta tacagaatgc cagcttcgag caggagctgg gtatatcctt gaagacttca
     6121 atgaagccca gtgtaacaca gcctaccagt gtctcctaat tgcggaccag cactgtcgaa
     6181 cccggaagta cttcctgtgc cttgccagtg gcattccttg tgtgtctcat gtctgggtcc
     6241 atgacagttg ccatgccaac caacttcaaa actatcgtaa ttatctgctg cctgctgggt
     6301 atagccttga agagcaacga attctggatt ggcaaccccg tgaaaaccct ttccagaatc
     6361 tgaaggtcct cttggtgtca gatcaacaac agaacttctt ggagctctgg tctgagatcc
     6421 ttatgactgg aggggcagcc tctgtgaagc agcaccattc aagtgcccac aacaaagaca
     6481 ttgctttagg ggtatttgat gtggtggtga cagacccctc atgcccagcc tcggtgctca
     6541 agtgtgctga agccttgcaa ctgcctgtgg tatcacaaga atgggtgatc cagtgcctca
     6601 ttgttgggga gagaattgga ttcaagcagc atccaaaata taaacatgat tatgtttctc
     6661 acggatccga ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg
     6721 atgacgatga caagtagtga gggcccgata tctcgacaat caacctctgg attacaaaat
     6781 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     6841 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     6901 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     6961 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7021 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     7081 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     7141 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     7201 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     7261 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     7321 gatctccctt tgggccgcct ccccgcctgg aaacggggga ggctaactga aacacggaag
     7381 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     7441 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     7501 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     7561 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     7621 catagcagat ctgcgcagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     7681 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     7741 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     7801 tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga
     7861 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     7921 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     7981 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tggggatttc
     8041 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     8101 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8161 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     8221 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     8281 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8341 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     8401 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     8461 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     8521 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     8581 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     8641 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     8701 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     8761 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     8821 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     8881 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     8941 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     9001 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     9061 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     9121 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     9181 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     9241 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     9301 ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     9361 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     9421 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     9481 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9541 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9601 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     9661 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     9721 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     9781 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     9841 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     9901 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9961 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    10021 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    10081 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    10141 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    10201 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
    10261 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    10321 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    10381 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    10441 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
    10501 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    10561 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    10621 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    10681 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    10741 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    10801 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    10861 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10921 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10981 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    11041 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    11101 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    11161 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    11221 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    11281 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    11341 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    11401 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    11461 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    11521 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    11581 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    11641 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    11701 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    11761 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
    11821 cccctatggt cgactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
    11881 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
    11941 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gg
//
