LOCUS       V044076                12132 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044076
VERSION     V044076
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..6812
                     /label="Tnc(NM_053861)"
                     /note="Tnc(NM_053861)"
                     /gene="Tnc"
     CDS             6819..6884
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    6908..7496
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7562..7610
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7812..8240
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8254..8583
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8650..9444
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9620..9741
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9790..9806)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9814..9830
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9838..9868)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9883..9904
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10192..10780)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10951..11811)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11812..11916)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatggg ggccgtgacc tggctattgc
      781 caggcatctt cctagctttg tttgccctca ctcctgaagg tggggtcctc aagaaagtca
      841 tcaggcacaa gcgagagagt gggctgaact tgaccttgcc agaggagaat cagccagtgg
      901 tgttcaacca tatctacaac atcaagttgc ccatgggttc tcagtgctca gtggatctag
      961 agtcaacgag tggagagaaa gacctgagcc ccacaccaga gtccagtgga agcttccagg
     1021 agcatacagt ggatggggaa aatcagattg tgttcacaca ccgcatcaac atccctcgtc
     1081 gggcctgtgg ctgtgccgca gctccagatg tgaaggagct cctgagcaga ctggaggaac
     1141 tggagatgtt ggtatcttct ctaagggagc agtgcaccat gggtacaggc tgttgcctcc
     1201 aacctgcaga aggccgtctg gacagcaggc ccttctgcag cggcaggggt aacttcagtg
     1261 ctgaaggttg tggctgtgtc tgtgaaccag gctggaaagg tcccaactgc tctgagcctg
     1321 aatgccctgg aaactgtaat ctcagaggcc agtgccttga tggacagtgt atctgtgacc
     1381 agggtttcac tggggaagac tgcagccagc tagcctgtcc caatgactgc aatgaccagg
     1441 gcaagtgtgt gaatggggtc tgtgtgtgct tcgaaggcta tgccggcctt gactgtggcc
     1501 tggaagtctg cccagtgccg tgcagcgagg aacacgggat gtgtgtggat ggcaggtgtg
     1561 tgtgcaaaga tggctttgct ggtgaagact gcaatgagcc cctttgcttc aacaactgca
     1621 acaaccgtgg gcggtgtgtg gagaacgaat gcgtctgtga tgagggcttc acgggcgaag
     1681 actgcagcga gctcatttgc cccaatgact gcttcgaccg aggtcgctgc atcaacggca
     1741 cctgctactg tgaagaaggt ttcacaggtg aagactgtgg tgagctcacc tgccccaaca
     1801 actgtcaggg ccatggccag tgtgaagagg gacagtgtgt ttgcaacgag ggctttgcag
     1861 gggcagactg cagtgaaaag cggtgtcccg cagactgtca ccaccatggc cgctgtctca
     1921 acggacagtg tgagtgtgac gatgggttca caggggctga ctgtggggaa ctccagtgcc
     1981 ccaatggctg cagtgggcat ggccgctgtg tcaatgggca gtgtgtgtgt gacgagggct
     2041 acaccggaga agactgtagc caacggcgat gccccaatga ctgccacaac cggggtcact
     2101 gtgtacaggg caaatgcata tgtgagcaag gcttcaaagg ctttgactgt agtgagatga
     2161 gctgtcccaa tgactgccac cagcatggcc gctgtgtgaa tggcatgtgc atctgtgatg
     2221 atgactacac tggggaagac tgcagagacc gccgctgtcc ccgggactgt agccagaggg
     2281 ggcgctgtgt ggacggacag tgcatttgtg aggatggttt cactggttct gactgtgctg
     2341 agctctcctg ccctggtgac tgccacggcc atggccgctg tgtgaatggc caatgcatct
     2401 gccacgaggg cttcaccggc aaagactgca aagagcaaag gtgccccagt gactgccacg
     2461 gccaaggccg ctgtgaggac ggccaatgta tctgtcatga gggcttcaca ggcctggact
     2521 gtgggcagcg ctcctgtccc aatgactgca gcaaccaggg acaatgtgtg gcaggccgct
     2581 gcatctgcaa tgaaggctac acaggggtag actgctctga ggtgtcccct cccaaagacc
     2641 ttattgtgac agaagtaaca gaggagactg taaatctggc atgggacaat gagatgcggg
     2701 tcactgagta cctcattatg tacacaccca cccatgctga tggcctggag atgcagttcc
     2761 gtgtgcctgg ggaccagaca tctaccacca tccgggagct ggagccagga gtggagtact
     2821 tcattcatgt gttcgccatc ttggagaaca agaggagcat ccctgtcagt gccagagttg
     2881 ccacctactt gcctgcacct gaaggcctaa aattcaagtc tatcaaggag acatctgtgg
     2941 aagtagagtg ggatcctctg gacatcgctt ttgaaacgtg ggagatcatt ttcagaaata
     3001 tgaacaaaga agatgaggga gagatcacaa aaagcttgag gaggccagag acctcctacc
     3061 gccaaactgg cctggctcct ggccaagaat atgaaatatc tcttcacatt gtgaaaaaca
     3121 acacccgagg ccctggcttg aagaaagtga ccacaactcg cctggatgcc cccagccaaa
     3181 ttgaggtgag agacatcaca gacactacag cactgatcac ctggtccaag cccttggctg
     3241 aaattgatag cattgagctc tcctatggca tcaaggatgt gcctggagac cggaccacca
     3301 tcgacctcac acatgaagac aaccagtact ccatcgggaa cctgaaacct gacacggagt
     3361 atgaggtgtc cctcatctcc cgcagagtgg acatggcaag caaccctgcc aaggagacct
     3421 tcatcacagg cctggatgct cccaggaatc tccgtcgcgt ctcacagaca gacaacagca
     3481 tcaccttgga gtggaggaat gtcaaggcag acattgatag ttatagaatt aagtatgcac
     3541 ctatctctgg aggtgaccat gctgagatag atgttccaaa gagccagcaa gccacaacca
     3601 aaaccacact cacaggtcta aggccaggaa ctgaatatgg agtgggcgtc tctgctgtta
     3661 agggagacaa ggagggcgat ccagcaacca tcaatgcagc cacagaaatt gatgcaccca
     3721 gggacttaca ggtgtctgag accacacaag acagtctgac tttattctgg aagacacctc
     3781 tggccaagtt tgatcgttac cgcctcaact acagcctccc cacaggccag tcaatagaga
     3841 ttcagctgcc aaaggatgcc acctcccatg tcctgacaga cctggagcca gggcaagaat
     3901 acactgttct cctcaccgct gagaagggca gacataagag caagcctgcg cgtgtgaagg
     3961 catccacaga agaagtacct tctctggaaa atctcaccgt gaccgaggcc ggctgggatg
     4021 gcctcagact caactggact gcagatgacc tggcctatga gtactttgtt attcaggtac
     4081 aggaagccaa caaggtggag actgctcaca acttcacagt acttggtaac ctccggactg
     4141 cagacatccc gggcctcaag gctgccactc cttatagagt ctccatttat ggggtagctc
     4201 ggggctataa aacacccgtg ctctctgctg agaccttcac agggaaaact cccagtttgg
     4261 gagaggtgac tgtggctgag gtgggctggg atgccctcaa gctcaactgg actgctccag
     4321 aaggagccta tacgaacttt ttcattcagg tgctagaggc tgacatgacc cagactgtcc
     4381 agaacctcac agtcccaggg ggactgaggt cagtggacct acctgggctc aaagcagcca
     4441 cccgctatta catcaccgtt cgaggggtca cccaggactt cagcacagcc cctctctctg
     4501 ttgaggtctt gacagaggag atccctcaac tgggaggctt gtccgtgact gaggtcagct
     4561 gggacagcct tacactcaac tggaccacag atgatctggc ctataaacac tttatcattc
     4621 aggtgcagga ggccaacaat gtagaggctg ctcagaacct cacagtatct ggtagcctca
     4681 gagttgtgga catcccaggc ctcaaggctg atacccctta tagagtctcc atctatgggg
     4741 tgatccaggg ctatagaaca ccaatgctct ctgctgatgt ctccacagcc aaagaacctg
     4801 aaattggaaa cttaaatatt tctgatgtaa ctcctgagag cttcaatctc tcctggacag
     4861 ctaccgacgg gatcttcgac atgtttacta tcgaaattat tgattctaat aggttgctgc
     4921 agacagcaga acataatata tctggtgctg aacgaactgc ccacatctcg ggccttccac
     4981 ctagtactga tttcattgtc tacctctctg gaattgctcc cagcatccgt accaaaacca
     5041 tcagtaccac agccaccaca gaagctgaac cggaagttga caaccttcta gtttcagatg
     5101 ctactccaga cggtttccgt ctgtcctgga ctgctgatga agggatattc gacagttttg
     5161 ttatcaggat cagagatacc aaaaagcaat ctgaaccaca agaaataacc ctcccttccc
     5221 ccgaccgtac cagggacata acaggtctca gagaggccac tgagtacgaa attgaactct
     5281 atggaataag ccgtggaagg cgatcccagc cagtcagtgc catagcaaca acagccatgg
     5341 gttctccgaa ggaaatcatg ttctcagaca tcactgaaaa tgcagccaca gtcagctgga
     5401 gagcaccgac tgctcaagtg gagagtttcc ggattactta tgtgcctgta acaggaggtc
     5461 ccccatccat ggtgaccgtg gatgggacgg atactgagac ccgactggtg aggctcaccc
     5521 ctggagtgga gtaccacgtc agtgtgattg ccatgaaggg atttgaagaa agtgatccgg
     5581 tctcggggtc tctaatcaca gctctcgatg gtccatctgg ccttctgaca gccaacatca
     5641 cagactcaga agccttggcc atgtggcagc cagccattgc cactgtagac agttatgtca
     5701 tctcctacac aggggagaga gtgccagaaa ttacccgcac agtgtctggg aatacagtgg
     5761 agtttgaact acatgacctg gagcctgcca cagagtacac actgagtgtc tttgcagaga
     5821 aaggtcacca gaagagttct accatcgcta cgaaatttac cacagacctg gattccccaa
     5881 gagaattgac tgctactgag gttcagtcag aaactgccct cctcacctgg agacctcccc
     5941 gggcatcggt cactggatac ctcctggtct atgagtctgt ggatggtaca gtcaaggaag
     6001 tcattgtggg gcctgacacc acctcctaca gcctggcaga cctgagccca tccacccact
     6061 acacagcaag aatccaggca ttgagtgggt ccctgaggag caaactgatc caaaccatct
     6121 tcaccacaat tggactccta tatccattcc ctagggattg ctctcaagca atgttgaatg
     6181 gcgacaccac ctctggcctc tataccatct atataaatgg tgacaagact caagcactgg
     6241 aagtctactg tgatatgacc tctgatggag gtggatggat cgttttcctg agacgcaaaa
     6301 atggacgtga ggacttctat cgaaactgga aggcctatgc cgctgggttt ggagaccgca
     6361 gagaagaatt ctggcttgga ctggataacc tgagcaaaat cacagcccaa gggcaatatg
     6421 agctccgggt ggacctacaa gaccatgggg agtcagccta tgctgtgtat gacaggttca
     6481 gtgttggaga tgccaagagt cgctacaagc tgaaggtaga aggatacagt ggaacggcag
     6541 gtgactccat gaactatcac aatggtagat ccttctctac ctatgacaag gacacagact
     6601 cagccatcac caactgtgcc ctgtcctaca aaggagcttt ctggtataag aactgtcatc
     6661 gtgtcaacct gatgggcaga tatggggaca ataatcacag tcagggtgtt aactggttcc
     6721 actggaaggg ccatgagtac tcaatccagt ttgcggagat gaaactaaga cccagcaact
     6781 tccgaaatct ggaaggcagg cgtaagcggg caggatccga ctacaaagac catgacggtg
     6841 attataaaga tcatgacatc gactacaagg atgacgatga caagtagtga gggcccgata
     6901 tctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6961 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     7021 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7081 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7141 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7201 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7261 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     7321 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7381 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7441 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     7501 aaacggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     7561 acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg
     7621 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     7681 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     7741 cgcagccaac gtcggggcgg caggccctgc catagcagat ctgcgcagct ggggctctag
     7801 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     7861 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     7921 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg
     7981 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     8041 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     8101 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     8161 ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta
     8221 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     8281 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     8341 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     8401 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8461 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8521 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     8581 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     8641 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     8701 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     8761 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     8821 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     8881 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     8941 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     9001 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     9061 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     9121 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     9181 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     9241 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     9301 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     9361 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     9421 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc
     9481 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     9541 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     9601 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     9661 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     9721 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     9781 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     9841 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     9901 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     9961 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    10021 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    10081 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    10141 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    10201 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    10261 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    10321 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    10381 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    10441 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    10501 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    10561 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    10621 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    10681 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    10741 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    10801 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    10861 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    10921 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    10981 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    11041 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    11101 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    11161 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    11221 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    11281 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    11341 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    11401 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    11461 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    11521 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    11581 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    11641 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    11701 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    11761 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    11821 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    11881 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    11941 tgacgtcgac ggatcgggag atctcccgat cccctatggt cgactctcag tacaatctgc
    12001 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
    12061 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
    12121 aatctgctta gg
//
