LOCUS       V018867                 7622 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018867
VERSION     V018867
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             665..2281
                     /label="TRIM26(NM_003449)"
                     /note="TRIM26(NM_003449)"
                     /gene="TRIM26"
     CDS             2288..2314
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2318..2344
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2348..2374
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    2398..2986
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3052..3100
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3302..3730
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3744..4073
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4140..4934
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5110..5231
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5280..5296)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5304..5320
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5328..5358)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5373..5394
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5682..6270)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6441..7301)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7302..7406)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgc
      661 caccatggcc acgtcagccc cactacggag cctggaagag gaggtgacct gctccatctg
      721 tcttgattac ctgcgggacc ctgtgaccat tgactgtggc cacgtcttct gccgcagctg
      781 caccacagac gtccgcccca tctcagggag ccgccccgtc tgcccactct gcaagaagcc
      841 ttttaagaag gagaacatcc gacccgtgtg gcaactggcc agcctggtgg agaacattga
      901 gcggctgaag gtggacaagg gcaggcagcc gggagaggtg acccgggagc agcaggatgc
      961 aaagttgtgc gagcgacacc gagagaagct gcactactac tgtgaggacg acgggaagct
     1021 gctgtgcgtg atgtgccggg agtcccggga gcacaggccc cacacggccg tcctcatgga
     1081 gaaggccgcc cagccccaca gggaaaaaat cctgaaccac ctgagtaccc taaggaggga
     1141 cagagacaaa attcagggct tccaggcaaa gggagaagct gatatcctgg ccgcgctgaa
     1201 gaagctccag gaccagaggc agtacattgt ggctgagttt gagcagggtc atcagttcct
     1261 gagggagcgg gaggaacacc tgctggaaca gctggcgaag ctggagcagg agctcacgga
     1321 gggcagggag aagttcaaga gccggggcgt cggggagctt gcccggctgg ccctggtcat
     1381 ctccgaactg gagggcaagg cgcagcagcc agctgcagag ctcatgcagg acacgagaga
     1441 cttcctaaac aggtatccac ggaagaagtt ctgggttggg aaacccattg ctcgagtggt
     1501 taaaaaaaag accggagaat tctcagataa actcctctct ctgcaacgag gcctgaggga
     1561 attccagggg aagctgctga gagacttgga atataagaca gtgagcgtca ccctggaccc
     1621 acagtcggcc agtgggtacc tgcagctgtc agaggactgg aagtgcgtga cctacaccag
     1681 cctgtacaag agtgcctacc tgcaccccca gcagtttgac tgtgagcctg gggtgctagg
     1741 cagcaagggc ttcacctggg gcaaggtcta ctgggaagtg gaagtggaga gggagggctg
     1801 gtctgaggat gaagaagagg gggatgagga ggaagaggga gaagaggagg aggaggaaga
     1861 ggaggccggc tatggggatg gatatgacga ctgggaaacg gacgaagatg aggaatcgtt
     1921 gggcgatgaa gaggaagaag aggaggagga agaggaggaa gttctggaaa gctgcatggt
     1981 gggggtggct agagactctg tgaagaggaa gggagacctc tccctgcggc cagaggatgg
     2041 cgtgtgggcg ctgcgcctct cctcctccgg catctgggcc aacaccagcc ccgaggctga
     2101 gcttttccca gcactgcggc cccggagagt gggcatcgcc ctggattatg aagggggcac
     2161 cgtgactttc accaacgcag agtcacagga actcatctac accttcactg ccaccttcac
     2221 ccggcgcctg gtccccttcc tgtggctcaa gtggccagga acacgcctcc tgctaagacc
     2281 cctcgagtac ccatacgacg tacctgatta tgcaacttat ccttatgatg tacctgatta
     2341 tgctagctat ccttatgatg tcccagacta cgcatagtga gggcccgata tctcgacaat
     2401 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2461 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2521 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     2581 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     2641 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     2701 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     2761 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     2821 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     2881 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     2941 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aaacggggga
     3001 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     3061 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     3121 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     3181 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     3241 gtcggggcgg caggccctgc catagcagat ctgcgcagct ggggctctag ggggtatccc
     3301 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     3361 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     3421 acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt
     3481 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     3541 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     3601 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     3661 taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     3721 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     3781 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     3841 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3901 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     3961 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     4021 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     4081 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
     4141 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     4201 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     4261 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     4321 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     4381 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     4441 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     4501 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     4561 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     4621 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     4681 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     4741 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     4801 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     4861 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     4921 acgagttctt ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac
     4981 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5041 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5101 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5161 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5221 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     5281 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     5341 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     5401 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5461 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5521 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5581 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5641 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5701 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5761 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5821 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     5881 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5941 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6001 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6061 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6121 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6181 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6241 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6301 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6361 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6421 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6481 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6541 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6601 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6661 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6721 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6781 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6841 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6901 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6961 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7021 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7081 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7141 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7201 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7261 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7321 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7381 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     7441 ggatcgggag atctcccgat cccctatggt cgactctcag tacaatctgc tctgatgccg
     7501 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     7561 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     7621 gg
//
