LOCUS V019388 6308 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019388 VERSION V019388 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 683..967 /label="Sumo2(NM_133354)" /note="Sumo2(NM_133354)" /gene="Sumo2" CDS 974..1000 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1004..1030 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1034..1060 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 1084..1672 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 1738..1786 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 1988..2416 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2430..2759 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2826..3620 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3796..3917 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3966..3982) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3990..4006 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4014..4044) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4059..4080 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4368..4956) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5127..5987) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5988..6092) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccgcca ccatggccga cgagaaaccc aaggaaggag tcaagactga 721 gaacaacgat catattaatt tgaaggtggc gggacaggat ggttctgtgg tgcagtttaa 781 gattaagagg catacaccac ttagtaaact aatgaaagcc tattgtgaac ggcagggttt 841 gtcaatgagg cagatcagat tccggtttga tgggcagcca atcaacgaaa cagacacacc 901 tgcacagttg gaaatggagg atgaagatac gattgatgtg ttccagcagc agactggagg 961 tgtctacctc gagtacccat acgacgtacc tgattatgca acttatcctt atgatgtacc 1021 tgattatgct agctatcctt atgatgtccc agactacgca tagtgagggc ccgatatctc 1081 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 1141 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 1201 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 1261 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 1321 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 1381 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 1441 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg 1501 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 1561 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 1621 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaaac 1681 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg 1741 caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt 1801 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc 1861 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca 1921 gccaacgtcg gggcggcagg ccctgccata gcagatctgc gcagctgggg ctctaggggg 1981 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 2041 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 2101 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc 2161 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 2221 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 2281 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 2341 gatttataag ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa 2401 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 2461 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 2521 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 2581 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 2641 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg 2701 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 2761 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt 2821 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 2881 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 2941 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 3001 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 3061 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 3121 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg 3181 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 3241 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 3301 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc 3361 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg 3421 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 3481 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 3541 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 3601 ttcttgacga gttcttctga gcgggactct ggggttcgcg aaatgaccga ccaagcgacg 3661 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 3721 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 3781 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc 3841 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 3901 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa 3961 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 4021 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 4081 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 4141 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 4201 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 4261 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 4321 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 4381 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 4441 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 4501 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 4561 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 4621 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 4681 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 4741 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 4801 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 4861 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 4921 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 4981 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 5041 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 5101 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 5161 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 5221 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 5281 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 5341 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 5401 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 5461 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 5521 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 5581 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 5641 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 5701 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 5761 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 5821 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 5881 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 5941 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 6001 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 6061 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 6121 gtcgacggat cgggagatct cccgatcccc tatggtcgac tctcagtaca atctgctctg 6181 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 6241 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 6301 tgcttagg //