LOCUS       V029375                 7506 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029375
VERSION     V029375
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3653
                     /label="Sulf2(NM_028072)"
                     /note="Sulf2(NM_028072)"
                     /gene="Sulf2"
     CDS             3672..3701
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3720..3743
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3763..3779)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3790..4412
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4561..5149)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5478..5525
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5757..6551)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6586..6943)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6945..7049)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      7076..7504
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggcaccccct ggcctgccac tatggctgct gtccaccgct ctcctctccc
     1081 tgctggctgg cagctcggcc ttcctctccc atccccgcct gaagggacgc ttccagaggg
     1141 accgcaggaa catccggccc aacatcatct tggtgcttac ggatgaccag gatgtggagc
     1201 tgggctccat gcaagtgatg aacaagacaa ggcgtatcat ggagcagggc ggggcgcact
     1261 tcatcaatgc cttcgtgact acaccaatgt gctgtccgtc tcgctcctcc attctcaccg
     1321 gcaagtacgt ccacaaccac aacacctaca ccaacaatga gaattgttcc tcgccctcct
     1381 ggcaggccca gcacgagagc cgcaccttcg ccgtgtatct caacagcaca ggctaccgga
     1441 cagctttctt cggaaaatac ctcaatgagt acaacggctc atacgtgccg cccggctgga
     1501 aggagtgggt cggcctactt aagaactccc gcttttataa ctacacactc tgccggaatg
     1561 gggtgaagga gaaacatggc tcagactact ccacggatta cctcacggat ctcatcacca
     1621 atgacagtgt gagcttcttc cgaacatcca agaagatgta cccacacagg cccgtgctca
     1681 tggtcatcag ccacgcggct ccccatggcc ccgaggactc agcaccccag tactcacggc
     1741 tcttccccaa tgcgtcccag cacatcacac cgagttacaa ctatgcaccc aacccagaca
     1801 agcattggat catgcgctac acgggaccca tgaagcccat tcacatggaa ttcaccaaca
     1861 tgctacaacg aaaacgccta cagaccctca tgtctgtgga tgactccatg gagacgatct
     1921 atgacatgct ggtggagacg ggggagctgg acaacacgta catcctgtac accgccgacc
     1981 acggctacca cattggccag tttgggctgg tgaagggcaa gtctatgccg tatgaattcg
     2041 acatcagagt cccgttctac gtgaggggcc ccaacgtgga agctggctct ctgaaccccc
     2101 acattgtcct caacattgac ctggccccca ccatactgga tatcgctgga ctggacatcc
     2161 ctgcagacat ggacgggaag tctattctca aactactgga ctcagagcgg ccagtgaacc
     2221 ggttccactt gaaaaagaag ctgagggtct ggcgagactc cttcctggtg gagagaggca
     2281 aactgctcca caagagggag ggtgacaaag tgaatgccca ggaggagaac ttcctgccca
     2341 agtaccagcg cgtgaaggac ctgtgtcagc gagctgagta ccagacagca tgcgaacagc
     2401 tggggcagaa gtggcagtgt gtggaggacg cttctgggac gctgaagctg cacaaatgta
     2461 aaggccccat gcggtttggt ggcggcggtg gcagcagagc cctctccaac ctggtgccca
     2521 agtatgacgg ccagagcagc gaggcctgca gctgtgacag tggcggtgga ggggactaca
     2581 aactgggcct ggctggacgc cgtaagctct ttaagaaaaa gtataagacc agctatgccc
     2641 ggaaccgctc catccgttcc gtggccatcg aggtggacgg tgagatatac cacgtaggct
     2701 tggatactgt gcctcagccc cgcaacctta gcaagccgca ctggccaggg gcccctgaag
     2761 accaagatga caaggatggt ggcagtttca gtggtactgg tggccttcca gattattctg
     2821 cccccaatcc catcaaagtg acccatcggt gctacatcct tgagaatgac acagtccagt
     2881 gcgacttgga cctgtacaag tccctgcagg cttggaaaga ccacaagctg cacatcgacc
     2941 atgagatcga aaccctgcag aacaaaatta agaaccttcg agaagtcagg ggtcacctga
     3001 agaagaagcg accggaagaa tgtgactgcc atagaatcag ttaccacagc caacacaaag
     3061 gccgtctcaa gcacaaaggc tccagcctgc accctttcag gaagggtctg caggagaagg
     3121 acaaggtgtg gctgctgcgg gagcagaaac gcaagaagaa actgcgcaag ctgctcaaac
     3181 ggctgcagaa caacgatacg tgcagcatgc ccggcctcac gtgctttacc cacgacaacc
     3241 accactggca gacggcgcca ctctggacgc tggggccgtt ctgcgcctgc accagcgcca
     3301 acaacaacac gtactggtgc ttgaggacca taaatgagac ccacaacttc ctcttctgcg
     3361 aatttgcaac cggcttcata gaatactttg acctcagtac agacccctac cagctgatga
     3421 acgcggtgaa cacactggac agggacgtcc ttaaccaact gcacgtgcag ctcatggagc
     3481 taaggagctg taaaggctac aagcagtgca acccccggac ccgcaacatg gacctggggc
     3541 ttagagacgg aggaagctat gaacaataca ggcagtttca gcgtcgaaaa tggccagaaa
     3601 tgaagagacc ttcttccaaa tcactgggac agctatggga aggttgggaa ggcacgcgta
     3661 cgcggccgct cgagcagaaa ctcatctcag aagaggatct ggcagcaaat gatatcctgg
     3721 attacaagga tgacgacgat aaggtttaaa cggccggccg cggtcatagc tgtttcctga
     3781 acagatcccg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
     3841 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     3901 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     3961 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     4021 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     4081 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     4141 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     4201 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     4261 gtccttctga ttttaaaata actataccag caggaggacg tccagacaca gcataggcta
     4321 cctggccatg cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc
     4381 gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc ggccagcggc gagcggtatc
     4441 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4501 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4561 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4621 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4681 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4741 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4801 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4861 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4921 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4981 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     5041 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5101 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5161 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5221 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5281 atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt
     5341 ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac
     5401 gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga
     5461 ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat
     5521 tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc
     5581 cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     5641 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     5701 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
     5761 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     5821 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     5881 gccaacgcta tgtcctgata gcgatccgcc acacccagcc ggccacagtc gatgaatcca
     5941 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     6001 agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc
     6061 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     6121 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     6181 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     6241 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     6301 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     6361 gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     6421 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     6481 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     6541 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag
     6601 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     6661 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     6721 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
     6781 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
     6841 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     6901 ccctaactga cacacattcc acagctggtt ctttccgcct caggactctt cctttttcaa
     6961 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7021 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     7081 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7141 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7201 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7261 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7321 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7381 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7441 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7501 aatttt
//
