LOCUS V033841 6790 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V033841 VERSION V033841 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..1449 /label="Srsf1(NM_173374)" /note="Srsf1(NM_173374)" /gene="Srsf1" CDS 1456..1482 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1486..1512 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1516..1542 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 1566..2154 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2220..2268 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 2470..2898 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2912..3241 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3308..4102 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4278..4399 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4448..4464) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4472..4488 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4496..4526) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4541..4562 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4850..5438) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5609..6469) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6470..6574) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgtc gggaggtggt 721 gtgatccgtg gcccggcggg gaacaacgac tgccgcatct acgtgggtaa cctacctccg 781 gatatccgaa ccaaggacat cgaggacgtg ttttacaaat acggcgccat ccgcgacatc 841 gacctgaaga accgccgcgg gggaccgccc ttcgccttcg ttgagttcga ggacccgcga 901 gacgcggaag atgcggtgta cggtcgcgac ggctacgact acgacggcta ccggctgcgg 961 gtagagtttc cccgaagcgg ccgcgggacc ggccgaggcg gcggcggggg tggaggcggc 1021 ggcgccccga gaggccgcta tggcccgccg tccaggcggt ccgagaacag agtggttgtc 1081 tctggactgc ctccgagtgg aagctggcag gacttaaagg atcacatgcg tgaggcaggt 1141 gatgtatgtt acgctgatgt ttaccgagat ggcactggtg tcgtggagtt tgtacggaaa 1201 gaagatatga cgtatgcagt tcgaaaactg gataacacta agtttagatc tcacgaggga 1261 gaaactgcct acatccgggt taaagttgat gggcccagaa gtccaagtta tggaagatct 1321 cgatctcgaa gccgtagtcg tagcagaagc cgtagcagaa gcaacagcag gagtcgcagt 1381 tactccccaa ggagaagcag aggatcacca cgctattctc cccgtcatag cagatctcgc 1441 tctcgtacac tcgagtaccc atacgacgta cctgattatg caacttatcc ttatgatgta 1501 cctgattatg ctagctatcc ttatgatgtc ccagactacg catagtgagg gcccgatatc 1561 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 1621 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 1681 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 1741 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 1801 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 1861 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 1921 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc 1981 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 2041 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 2101 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa 2161 acgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac 2221 ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg 2281 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg 2341 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg 2401 cagccaacgt cggggcggca ggccctgcca tagcagatct gcgcagctgg ggctctaggg 2461 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 2521 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 2581 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt 2641 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 2701 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 2761 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 2821 ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac 2881 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc 2941 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg 3001 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 3061 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 3121 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 3181 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa 3241 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc 3301 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag 3361 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg 3421 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa 3481 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc 3541 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc 3601 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga 3661 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa 3721 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct 3781 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat 3841 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt 3901 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta 3961 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga 4021 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg 4081 ccttcttgac gagttcttct gagcgggact ctggggttcg cgaaatgacc gaccaagcga 4141 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct 4201 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg 4261 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata 4321 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 4381 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt 4441 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 4501 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 4561 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 4621 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 4681 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 4741 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 4801 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 4861 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 4921 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 4981 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 5041 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 5101 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 5161 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 5221 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 5281 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 5341 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 5401 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 5461 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 5521 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 5581 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 5641 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 5701 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 5761 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 5821 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 5881 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 5941 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 6001 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 6061 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 6121 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 6181 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 6241 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 6301 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 6361 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 6421 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 6481 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 6541 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 6601 acgtcgacgg atcgggagat ctcccgatcc cctatggtcg actctcagta caatctgctc 6661 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta 6721 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa 6781 tctgcttagg //