LOCUS       V043972                14184 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043972
VERSION     V043972
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..8864
                     /label="Srrm2(NM_001368687)"
                     /note="Srrm2(NM_001368687)"
                     /gene="Srrm2"
     CDS             8871..8936
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    8960..9548
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    9614..9662
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      9864..10292
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        10306..10635
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             10702..11496
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    11672..11793
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(11842..11858)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11866..11882
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11890..11920)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11935..11956
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12244..12832)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13003..13863)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13864..13968)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgta caacgggatc gggctgccga
      781 cgccccgggg cagcggcacc aacggctacg ttcagcgaaa cctgtccctg gtgcggggcc
      841 gccgtggtga gcggcctgac tacaagggag aggaggaact gcggcacctg gaggctgccc
      901 tggtgaagcg acctaatcct gacatcctgg accacgagcg caagcggcgc gtggagctgc
      961 gatgcctcga gctggaggaa atgatggaag agcaggggta cgaagaacag caaattcagg
     1021 aaaaagtggc gacctttcga ctcatgttgc tggaaaagga tgtgaaccct ggggccaagg
     1081 aagaaacccc agggcagaga ccagtggtaa ctgagaccca tcagttggca gaactgaatg
     1141 agaagaagaa tgaacgactc cgtgctgcct ttggcatcag tgactcctat gtggatggca
     1201 gttcttttga tcctcagaga cgtgctcgag aagctaaaca aatagctcct gagcctccca
     1261 aaccatacag ccttgttcga gagaccagca gttctcgctc accaactcca aagcaaaaga
     1321 agaagaaaaa gaagaaagat agaggacgca ggtcagagag cagttctcct cgacgagaga
     1381 ggaagaagag ttcaaagaag aagaagcaca ggtcagaatc tgagtccaag aaacggaaac
     1441 acaggtctcc tactccaaag agcaaacgca aatcaaagga caagaagagg aaacggtctc
     1501 gaagtacaac accagctcct aagagccgtc gagctcatcg gtcaacttct gcggactctg
     1561 cttcttcctc tgatacttct cgtagtcggt ctcgaagtgc tgctgctaaa attcatacaa
     1621 cagccttgac tgggcaaagt cctccccttg cttcagggca tcaaggggag ggagatgcac
     1681 cttctgttga accaggtgcc accaacatac aacaacctag tagcccagct ccctctacaa
     1741 agcaatctag cagtccttat gaagacaaag ataagaaaga gaaatctgca gttcgaccta
     1801 gcccctctcc ggaaaggagc agcacagggc cagaactacc tgcccctact ccgctccttg
     1861 ttgagcaaca tgtcgactcc ccacgacccc ttgcagcaat cccgtccagt caggagccag
     1921 ttaacccctc atctgaggct tccccaaccc ggggctgttc accacctaag tctcctgaga
     1981 aacctcccca gtctacttcc tcagaaagtt gcccaccatc ccctcaacct accaaaggtt
     2041 ctcggcatgc cagctcttct cctgaaagtc ttaaacccac accagctcct gggtcccgtc
     2101 gagagatttc ttcttcaccc acatctaaga atcgctcgca tggcagagca aagcgggata
     2161 aatctcattc tcatacccca tctcatagag cagggaggtc tcgtagtcct gccactaaga
     2221 ggggacggtc tcgatctaga acccccacca agagaggtca ctccaggtcc cggtcccctc
     2281 agtggagaag gtcaagatct gcacagaggt ggggaaaatc tagaagtccc cagaggcgtg
     2341 gccgttcaag gtctcctcag aggccaggtt ggtccaggag cagaaatacc cagagaagag
     2401 gcaggtctag gtcagcaagg cgaggcagat cacattctag atctccagct actaggggca
     2461 gatcccgatc tagaacacca gctagaaggg gtaggtctcg gtccagaaca cctgccaggc
     2521 gaagatcacg atccagaaca cctgccaggc gtaggtctcg gtctagaaca ccagctcgca
     2581 ggggcaggtc cagatcaaga acacccgctc gccgcaggtc taggacacga tcaccagttc
     2641 gaaggaggtc tcggagtaga tcccaagcta ggagaagtgg taggtctagg tccagaacac
     2701 cagccaggag aagtggcagg tcaagatcta gaacaccagc caggagaggg aggtcccggt
     2761 ctagaacgcc agccagaaga agcgctcgat ctcggtctag aacgccagcc aggagaggga
     2821 ggtcacgatc cagaacacca gcacgacgac gatctcgaag tagaagtctt gtgagacgtg
     2881 gaagatctca ctctagaaca ccacaaagaa gaggacgatc tggctcatcc tcagagagga
     2941 agaacaaatc tagaacatct cagaggagaa gcagatccaa ctcaagccca gaaatgaaaa
     3001 aatctcacgt ttcctcaaga cggagcaggt ctctttcttc accacgatcc aaagcaaaat
     3061 ctttgaggcg aagcctttca ggttcctctc catgtcctaa acaaaagtca cagacaccaa
     3121 ccaggcgaag tcgttctggt tcttcaccac ctaaacaaaa gtcaaaaaca ccaccaagac
     3181 agagtcgttc aaattctcct cagcctaaag taaaatctgg aacaccacca agaccagggt
     3241 ctgtaacaaa catgcaggct gatgaatgca ctgcaacacc acagagacag agccattctg
     3301 aatcatcacc tgatggtgag gtgaaatcaa ggaccccatc aagacaaagc tgctctgggt
     3361 cttctcctcg agtgaaatct agcacacctc caagacagag cccatctagg tcgtcatctc
     3421 cacaacccaa agtgaagaca gtgatatcac caagaggaag aagccattct agctcctctt
     3481 ctccaagtcc tagtagagtg acatctagga cacctcagag gaaaagcaga tcaatatctc
     3541 cttgccccaa ggtagactct agattaagac atagccgttc tagatcctcc tcaccagatt
     3601 ccaagatgga actgggaaca ccactgagac actcaggatc tacatctcca tatctaaaaa
     3661 gcatgctcca gactccacca gatcaaaatc tttctggatc aaagtcaccc tgtccccaga
     3721 agtctaggga ttcaccaaca ggaagctctg gatcttttca cctctgtcca ggtgtaacac
     3781 ctagtagtat agtaccagga gagagctgtt ttagcgcctc atttgtgcaa caaaaaggac
     3841 acactcaaac ttggccagat acttcaagtc cagaagtcat gcagactcaa gtggaatctc
     3901 cattgctgca gagcaaatcc caaacatctc ctaagggtag cctttccaga tcttcatctc
     3961 cagtcactga gctgacagcc agatcaccag taaaacaaga taaaagtgaa atatcaacag
     4021 atccaaagct gaagtcagga atgtctcctg agcagagtaa gactaaacct gactctagca
     4081 tatatccttt agtagactct aaatcttttc tggtgcagag cagattggag ccttctgaat
     4141 taaaagagag actgggttta attcaagagg atgttgcatc atcttgtata ccaagagaca
     4201 aatttagtcc tacacaggat aggcctgagt cttccacagt actgaaagtc acacctagag
     4261 tactattaaa agaaagaagt ggagctggct cacctccagg caaaagagac caaaaaagtt
     4321 tattacctaa ttcaagtcaa gatgaactaa tggaagtaga aaagtctgag caacctttaa
     4381 gccaagttct acccagttta tctccagaac ataaagaaat gcctggaagt aacattgagt
     4441 catctcctga agtagaagaa aggcctgctg tattgtctgc tcttgaccaa agccagtcac
     4501 agccttcaaa agcagcagaa acccctgcag tggcttcatg ttggagtggg ccacaagttt
     4561 ctccagagca taaagaacta tctcattctc ccccaaggga gaatagcttt gagtcatctt
     4621 tagagtttaa aaactcaggc cctgtttcag aagtaaatac tggcttttct cctgaggtta
     4681 aagaagaatt gaatggatca ttccttaatc aaacagaggc agatccatct gtggacatga
     4741 aagaacagtc aaggtcttcc aggcgaagca gttctgagtt atccccagaa gtagtggaaa
     4801 aggtaggatt attttcaagt cagaaagtat cttcgcctgt acttgagact gtacaacaaa
     4861 gaacaccttc aagagaaaga agtagttctg catctcctga actgaaagat ggtttgccta
     4921 gaacgccatc taggagaagc cggtctgggt cttctccagg acttagagat gggtctggga
     4981 ctccttctag gcatagccta tctgggtcct ctcctgggat gaaagataca cctcaaaccc
     5041 cctccagggg acgaagtgaa tgtgactctt ctccagaacc aaaagcattg cctcagactc
     5101 ctagagcccg aagtcattct ccatcatccc cagagcgcaa caacaagagt gtcacccctc
     5161 agagagaaag aagtgggtca gaatcatcag tagaacagaa aaatctggct aggacctctc
     5221 cgggacaaag aagtcgatct gggtcttccc aagagcttga tggaaagccc agcgcgtccc
     5281 cacaggaaag aagtgagtca gactcttctc cagattccaa acccaagact cggactcctc
     5341 tcagacagag gagtcactct ggatcatctc cagaggttga cagcaaatct cgacactcac
     5401 ccaggctcag taggtctggc tcatctccgg aaatgaaaga taagccaaga gtgctacaga
     5461 gggctcagag tggtactgat tcttctcctg aacacaagat acctgctcct cgggccctgc
     5521 ccaggcatag tagatcaggt tcatcaagca aagagagagg tccttcccct gaaggaagca
     5581 gtagttctga gtcttctcca gaacacgccc ccaaatccag aactgctcga agaggttcca
     5641 ggtcctcaat agagccaaag acaaagtctc acacaccacc tcgacgccgg agctctcgat
     5701 catctcctga gctaaccagg aaggctagag tctctcgtag aagccggtct gcctcctcat
     5761 caccagaaat ccgctccaga actccaccaa gacgcagaag aagtccttca gtatcttcac
     5821 cagagccaac tgaaaagtca aggtcttcac ggaggaggcg ctcagtttct tctccccgta
     5881 ccaagacaac ttcgaggaga ggacggtctc cttcacccaa acctcgtgga ctccaaagat
     5941 cccgatcccg ctcacggaga gagaaaacca gaacaacccg acgcagagat agatctggat
     6001 catctcagtc aacatctcga agaagacaga ggagccggtc tagatcacga gttactcgga
     6061 gacggagggg tggctctggt taccattcaa gatcacctac cagacaggag agttctcgaa
     6121 cctcctctag acgcagaaga ggccgctccc ggacaccctt gaccagtcgg aagcgatctc
     6181 gatcaagaac atcaccagct ccttggaagc gctctagatc tcgagcctca ccagctactc
     6241 atcggcggtc caggtccaga acaccactga taagccgacg taggtccaga tctcggacct
     6301 cacctgtgag taggagacgg tcaaggtcag tgaataggcg tagatctcga tcaagagcat
     6361 ccccagtgag tcgaaggcga tccaggtcca gaacaccacc agtaactcgt cgtcgttcaa
     6421 gatccagaac accaactcgc cgtcgttctc gttctaggac tcctccagtg actcgcagaa
     6481 ggtccagatc caggactcca ccagtaacca ggaggcgatc tagaagcaga acttcacctg
     6541 ttactagaag aagatcaaga tccaggacat ccccagtcac ccggagaaga tcgagatctc
     6601 gaacatctcc agtcacccgg aggcggtccc gttcccgaac ctctccagtg acaagacgac
     6661 gctccaggtc tcgaactccg ccagcaattc gaagacgatc taggtctcgg acaccactgt
     6721 tgccacgcaa gcgctctcga agccgctcac cacttgctat ccgccgccgt tctaggtctc
     6781 gtactcctag ggcagctcga ggcaagcggt ccttaacaag atctcctcca gccattcgta
     6841 ggcggtctgc atctggaagt agttctgatc gttcacgttc tgccactcct ccagcaacaa
     6901 ggaatcattc tggatctcgg acacctcctg tagcactcag tagctctaga atgagttgct
     6961 ttagccgtcc tagcatgtca ccaactcctc ttgaccgatg tagatcacct ggaatgcttg
     7021 aaccccttgg aagcgctaga acgcccatgt ctgtgctaca gcaaacaggt ggctccatga
     7081 tggatggtcc aggtcctcgg attcctgatc acccaagatc atctgttcca gaaaaccatg
     7141 ctcagtctag aattgcactt gccttgacag ccatcagtct tggtactgcc cggccacctc
     7201 catccatgtc tgctgctggc cttgctgcaa gaatgtccca ggttccagca cctgtacctc
     7261 tcatgagcct tagaacagcc ccagctgcta accttgccag caggattcca gcagcatctg
     7321 cagcagctat gaaccttgct agtgccagga catctgctat tccggcatct gtgaaccttg
     7381 ctgactcaag aacaccagct gctgctgcag ctatgaactt agccagccct agaacagcag
     7441 tggctccttc agctgtgaac cttgcagatc ctcgaacccc tgcagcttca gctgtgaacc
     7501 tagcaggagc tagaacacca gctgcattag cagctttgag tctcacaggc tctgggacac
     7561 ctccaactgc tgcaaattac ccctccagtt ccagaacacc ccaggctcca acccccgcaa
     7621 acctggtggt gggtcctcga tctgcgcatg gcacagctcc tgtgaatatt gctggctcta
     7681 gaacccctgc aggcttggct cccacaaatc tctccagttc caggatggct ccagcattgt
     7741 ctggtgcaaa cctcaccagt cccagggtgc ccctttctgc ttatgatcgt gttagtggca
     7801 gaacctcacc tttaatgctt gatcgagcca ggtccagaac accaccatct gccccaagcc
     7861 agtctagaat gacctctgag cgtgagcggg ctccttcccc tgcttcaaga atggttcagg
     7921 cttcttcgca atctcttctc cctcctgcac aggatcggcc tcggtcccct gtgccatctg
     7981 ctttttcaga ccaatctcgt tcagttgtcc agaccactcc tgtagcaggg tctcagtccc
     8041 tttcatccgg gacagtagcg aagtccacat cctctgctag tgaccacaat ggcatgcttt
     8101 ctggccctgc ccctgggata tcccatgctg aaggtgggga gccacctgcc tctactgggg
     8161 cccagcagcc ttctacattg gctgctctgc aaccagctaa ggaacgtcgg agctcttcct
     8221 cctcttcatc atcatctagc tcctcctcct cttcatcatc ttcatcgtca tcctcctctt
     8281 cctctggctc cagttctagt gattcagagg gttccagcct tccagctcaa cctgaggtgg
     8341 cactgaaaag ggttcccagc cccaccccag tcccaaagga ggctattaga gagggacgtc
     8401 ctcaggaacc aaccccggcc aaaaggaaga ggcgctctag cagctccagt tccagctcct
     8461 cctcctcctc ctcttcctct tcttcctctt cctcctcctc ctcatcttcc tcctcctctt
     8521 cttcctcttc ttcctcctct tcttcctcct cttcctcctc cccctcccct gctaagcctg
     8581 gccctcaggc attacccaaa cctgcaagcc ccaagaagcc accccctggc gagaggaggt
     8641 ctcgaagtcc tcggaagcca atagactccc ttcgggattc ccgatccctc agttactcac
     8701 ccgttgagcg tcgacagccc tcgccccaac cttcaccaag ggatctacag agcagtgagc
     8761 gagtttcctg gagaggccag cgaggggaca gtcactctcc tggtcacaag aggaaggaga
     8821 cacctagtcc ccgttctaat cgtcaccgct cctccaggtc tccaggatcc gactacaaag
     8881 accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtagt
     8941 gagggcccga tatctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     9001 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     9061 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     9121 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     9181 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     9241 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     9301 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     9361 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     9421 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     9481 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     9541 ctccccgcct ggaaacgggg gaggctaact gaaacacgga aggagacaat accggaagga
     9601 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt
     9661 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     9721 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     9781 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag atctgcgcag
     9841 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     9901 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     9961 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
    10021 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
    10081 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
    10141 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
    10201 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa
    10261 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
    10321 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
    10381 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    10441 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
    10501 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    10561 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    10621 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
    10681 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
    10741 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
    10801 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
    10861 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
    10921 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
    10981 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
    11041 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
    11101 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
    11161 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
    11221 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
    11281 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
    11341 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
    11401 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
    11461 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat
    11521 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
    11581 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
    11641 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
    11701 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    11761 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
    11821 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
    11881 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
    11941 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
    12001 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
    12061 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    12121 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    12181 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    12241 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    12301 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    12361 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    12421 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    12481 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    12541 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    12601 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    12661 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
    12721 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    12781 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    12841 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    12901 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    12961 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    13021 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    13081 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    13141 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    13201 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    13261 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    13321 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    13381 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    13441 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    13501 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    13561 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    13621 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    13681 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    13741 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    13801 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    13861 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    13921 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    13981 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtcgactctc
    14041 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
    14101 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
    14161 aattgcatga agaatctgct tagg
//
