LOCUS       V023272                 5972 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023272
VERSION     V023272
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1004..2134
                     /label="Sox18(NM_009236)"
                     /note="Sox18(NM_009236)"
                     /gene="Sox18"
     CDS             2138..2167
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2186..2209
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2229..2245)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2256..2878
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3027..3615)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3944..3991
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4223..5017)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5052..5409)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5411..5515)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5542..5970
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccgcc accatgcaga gatcgccgcc
     1021 cggctacggc gcacaggacg acccgccctc ccgccgcgac tgtgcatggg cccctggaat
     1081 cggggccgct gctgaggcgc gcggcctccc tgtcaccaac gtctcgccca cctcgcccgc
     1141 ctccccgtcc agccttccgc ggagcccacc gcgcagcccc gaatcagggc gctatggctt
     1201 tggccgcgga gagcgccaaa ctgccgacga gttgcgcatt cggcggccca tgaacgcctt
     1261 catggtgtgg gcgaaggacg agcgcaagcg actggcgcaa caaaatccgg atctgcacaa
     1321 cgcagtactg agcaagatgc tgggcaaagc gtggaaggag ctgaacacgg cggagaagcg
     1381 gcccttcgtg gaagaggccg aacggctgcg tgtgcagcac ttgcgcgacc atcccaacta
     1441 caagtaccgg cctcgccgca aaaaacaggc gcgcaaggtc cggaggctgg agccgggcct
     1501 cttgctcccg ggcctcgtgc agccgtctgc gccgcccgag gccttcgctg cagcgtcagg
     1561 gtcagctcgc tccttccgcg agctacccac tctgggtgcg gagttcgatg gcttggggct
     1621 acccacgccc gagcgctcgc ctctggacgg cctggagcct ggcgaggcct ccttcttccc
     1681 accgcctttg gcgcccgagg actgcgctct gcgggctttc cgggcaccct atgcccctga
     1741 gctggcacgg gacccgagct tctgctacgg ggcgcccctg gctgaagcgc tcaggacagc
     1801 gccgcctgcc gcgccactcg caggtctcta ctatggcacc ctgggcactc cgggcccgtt
     1861 tcccaatcct ctgtcaccac cacctgagtc cccgtctctt gagggcacag agcaactgga
     1921 gcctaccgcc gacctttggg ccgatgtgga cctcaccgaa tttgaccagt atctcaattg
     1981 cagccggact cgaccggatg ccactacact cccctaccac gtggcactgg ccaaactagg
     2041 tccgcgcgcc atgtcctgtc cagaagagag cagcctcatt tctgcgctgt ctgatgctag
     2101 cagcgcggtc tattacagtg cttgcatctc aggcctcgag cagaaactca tctcagaaga
     2161 ggatctggca gcaaatgata tcctggatta caaggatgac gacgataagg tttaaacggc
     2221 cggccgcggt catagctgtt tcctgaacag atcccgggtg gcatccctgt gacccctccc
     2281 cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa
     2341 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg
     2401 gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt
     2461 gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg
     2521 ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca
     2581 ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg
     2641 tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac
     2701 aggcgtgaac cactgctccc ttccctgtcc ttctgatttt aaaataacta taccagcagg
     2761 aggacgtcca gacacagcat aggctacctg gccatgccca accggtggga catttgagtt
     2821 gcttgcttgg cactgtcctc tcatgcgttg ggtccactca gtagatgcct gttgaattgg
     2881 gtacgcggcc agcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2941 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3001 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3061 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3121 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3181 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3241 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3301 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3361 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3421 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     3481 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3541 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3601 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3661 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3721 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta acctgaggct
     3781 atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg
     3841 gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg tctcggtggg
     3901 gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca
     3961 ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc cggtattgtc
     4021 tccttccgtg tttcagttag cctcccccta gggtgggcga agaactccag catgagatcc
     4081 ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca
     4141 tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc
     4201 atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc
     4261 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     4321 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcga tccgccacac
     4381 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     4441 agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc
     4501 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     4561 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     4621 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     4681 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
     4741 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg
     4801 tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca
     4861 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     4921 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     4981 ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct
     5041 cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat
     5101 agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg
     5161 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg
     5221 actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
     5281 ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     5341 gctggggagc ctggggactt tccacaccct aactgacaca cattccacag ctggttcttt
     5401 ccgcctcagg actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5461 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5521 ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     5581 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5641 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     5701 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5761 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     5821 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     5881 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     5941 agctgattta acaaaaattt aacgcgaatt tt
//
