LOCUS V044669 9186 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V044669 VERSION V044669 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 678..696 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 756..3866 /label="Senp7(NM_025483)" /note="Senp7(NM_025483)" /gene="Senp7" CDS 3873..3938 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 3962..4550 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 4616..4664 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4866..5294 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5308..5637 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5704..6498 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6674..6795 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6844..6860) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6868..6884 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6892..6922) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6937..6958 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7246..7834) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8005..8865) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8866..8970) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct 661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta 721 aacttaagct tggtaccgag ctcgaattcg ccaccatgga cagggccagg cctgggcgtc 781 ggcgagcttc atcagagatt gttacagagg gaaaacggaa aaagtcatca ccagctgatt 841 tacagaagat aacaaagttg ttaactgtaa agtcagagga tgttcttgct cagtcaccat 901 tgtccaaact cagaggctca gaatgctggt ggacaagaag cctaagaaat aaagtcatct 961 gtctagacca caaaaaacca aaagctgccc gtgggtgtcc tcctaaggga ttaccaaaaa 1021 ggcatctcag agttatgttg acgaatgttc tatggacgga cttaggacga gaattcagaa 1081 agaccctgcc tagaaaggat gctaatttat gtgctcccag caaggtgcaa tcagactcat 1141 tgccttcgac atctgttgac agcatagaga catgtcaaag attagatcct cttcaccaaa 1201 gccttaattt atccgaaagg acacccagag ttatactgac ggatatccgg caaacagaat 1261 taggaagaaa atatttaaag atcccacctg taactgaggc cagtttgagt gatacagcca 1321 acctgaaatc agagcaactt tcttcatcat ctgatggcag cttagagtct tgtcagagtg 1381 taaatcatca caagagcttt ttatctgaaa gtggtcccaa accaagtagg acaggtgacg 1441 ttcctgcaaa ggaggctgca tgtgggggac agaagcaggg tgatgatgga ggagtcactc 1501 ctgagatggc tgctcctcat cctaaagact ttaatactgg aaataaaggt tgtgattatc 1561 ttgaagaggg gaccagcaac aagaatactt catattctta ttcggaaatg gaccatactc 1621 cagtttccag gaagagaaag aaaaggggta gaagtaattt tcatgattct cataattcta 1681 agacttcttt ggataagcca acagaacata caaaagaaga agaaaatgac tcttcagtct 1741 cccgtaagct tgaagagtcc ggtgaagact ctcaccagga cccagcacca cccgaaggac 1801 tcgcgccaga atctttagag agcgaggcca ctaacctgcg ctcattcgct gctggtcagg 1861 agccagatgc tagcgctgcc tctggaaggg cctcttcacc caacaaaagc ttggagagct 1921 ctgcttctag tgaagtgtcg gagaattctt ccgtggctgt gaagggtgag gcgttgactc 1981 taaaagaagc atcccctccg ggtggaagca gtgaagagag ccagttgttg atctcagctg 2041 agccaattgt agtttccagt gatgaagaag gaccggttga acataagaat tcagtaattc 2101 taaaattaca gcctcctcat gagattatga gtgaaaatca gggcacttct gacccacagt 2161 tgtcagagct gacactcggt gcatgtgaat ctgtacaggt aacatctgaa ttatttccat 2221 acaatcctga tgtagaaaac atttcctgta ttaaatctaa tagtgaaatg gatctgaaat 2281 tggatttcat atttacatgt gtgtatattg gtaaaataaa aggaactcca aaaggttgtg 2341 tcacgttcac aaagaagtat attaaaatcc cgtttcaagt gtccacaaat gagatttccc 2401 tgacagtgga caccgcacgt ttaaagaggt tcgggttatg ggaaagcaag gatgaggacc 2461 acagcaagag gagccacgct atccttttcc tctggctttc ctcagactac cttcaagaca 2521 ttcagaccca gctagaaaac cccatgctaa gccagcaatc aaaagccaat gaatttattt 2581 tccttgagtt aaacagttcc atttcacaaa gagaagaatt gaaattgaaa gatattatga 2641 tggaaatcag tacaaaaaat ggaaatttac acctgtcttg tccattgccc tgggtccagg 2701 cacttccttt gtttcaggat ctctctccac aagaaatctc ttttcttcac tattactatg 2761 cttcagcttc tgctctcccc actgctgctg gggcagacat gaagaagaag tcagtgtctc 2821 agccctcaaa ttcagacacc atcaagccta cttacacctt cctacataaa caaagtagtg 2881 gttgctactc gctctctatt acttcaagtc cagaggagga atggcaggaa gtcaggaaca 2941 cgggacccgt tcagaagttg attgtttatc ctccaccacc tactaaggga ggcttgggag 3001 taactaatga agatctagag tgtttagaag aaggagaatt tcttaacgat gtaatcattg 3061 acttttacct taagtatctt ctattggaga aggcatcaga tgaacttgtt gaacgaagtc 3121 acatttttag tagctttttc tataaatgct tgacaagaaa ggaaaataat ttaacagaag 3181 ataatccaga tctatcagtg gctcagagga gacaccgaag agtgagaaca tggactcgtc 3241 atataaacat ttttaataaa gactacatct ttgtacctgt taatgaatcg tctcactggt 3301 atcttgcagt catttgtttc ccatggctag aagaggctgt gtatgaagat tgtccacaaa 3361 ctgtgtccca acagttccag ggtcagcagt ctcagcatga ccacaaaatg acagataatg 3421 atccacatac cacttcaact gtgtccacga gtgcagagga ttcccaaagt accgaggtga 3481 atatgtcagt accaaagaaa atgtgtaagc ggccatgtat tctcatacta gactccctga 3541 aagctgcttc tatacaaaac acagttcaga acttacgaga gtatttggag gtagagtggg 3601 aggtgaaacg aaaaacgcac cgtgaattca gtaaaaccaa tatggtcgac ctgtgcccca 3661 aagtccctaa gcaggataac agcagtgact gtggagtcta tttgttgcag tatgtggaga 3721 gctttttcca ggatcccatt gttaactttg aacttccaat tcacttggag aaatggtttc 3781 ctcgccatgt aataaagacc aaacgggaag atattcgaga gctcattttg aaacttcact 3841 tacagcaaca gaagggcggc agctgcggat ccgactacaa agaccatgac ggtgattata 3901 aagatcatga catcgactac aaggatgacg atgacaagta gtgagggccc gatatctcga 3961 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 4021 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 4081 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 4141 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 4201 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 4261 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 4321 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 4381 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 4441 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 4501 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaaacgg 4561 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca 4621 ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg 4681 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg 4741 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc 4801 caacgtcggg gcggcaggcc ctgccatagc agatctgcgc agctggggct ctagggggta 4861 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 4921 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 4981 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt tagggttccg 5041 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 5101 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 5161 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 5221 tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 5281 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 5341 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 5401 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 5461 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 5521 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc 5581 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag 5641 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt 5701 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 5761 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 5821 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 5881 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 5941 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 6001 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 6061 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 6121 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 6181 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc 6241 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 6301 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 6361 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 6421 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 6481 cttgacgagt tcttctgagc gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc 6541 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg 6601 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt 6661 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 6721 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 6781 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc 6841 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 6901 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 6961 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 7021 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 7081 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 7141 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 7201 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 7261 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 7321 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 7381 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 7441 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 7501 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 7561 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 7621 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 7681 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 7741 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 7801 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 7861 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 7921 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 7981 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 8041 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 8101 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 8161 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 8221 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 8281 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 8341 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 8401 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 8461 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 8521 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 8581 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 8641 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 8701 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 8761 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 8821 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 8881 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 8941 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 9001 cgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat 9061 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 9121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 9181 cttagg //