LOCUS V018888 9854 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018888 VERSION V018888 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 683..4510 /label="Scap(NM_001001144)" /note="Scap(NM_001001144)" /gene="Scap" CDS 4517..4606 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" misc_feature 4630..5218 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 5284..5332 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5534..5962 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5976..6305 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 6372..7166 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 7342..7463 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(7512..7528) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7536..7552 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7560..7590) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7605..7626 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7914..8502) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8673..9533) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9534..9638) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccgcca ccatgaccct gactgaaagg cttcgtgaga agatatctca 721 ggccttctac aaccatgggc tgctctgcgc atcctatcca attcccatca tcctcttcac 781 aggactctgc atcttagcct gctgctaccc gctgctgaag ctccccttgc ctggaacggg 841 acctgtggaa ttctccacgc ctgtgaaggg ttactcgccc ccgcctgcgg actctgacca 901 caaacaagga gagcccagcg agcagccaga gtggtatgtg ggtgcccccg tggcgtacat 961 ccaacagata tttgtgaagt catcggtgtc tccctggcac agaaatcttc tggcagtcga 1021 tgtgttccgg tcacctctgt cccgagcatt ccaactggtg gaagagatcc ggaaccatgt 1081 gctgagagac agctcaggga ccaagagcct ggaggaggtt tgcctgcagg tgacagacct 1141 gctgccaggc ctcaggaaac tccggagcct acttcccgaa catggctgcc tgctgctgtc 1201 ccctgggaac ttctggcaga atgattggga gagattccat gccgaccctg acatcattgg 1261 gaccatccat caacatgagc ccaaaactct acagacatca gccacactca aagacttgct 1321 gtttggtgtt cctgggaagt acagtggggt gagcctctac acaaggaaaa ggatggtctc 1381 ctacaccatc accctggtct tccagcgcta ccatgccaag tttctgagca gcctacgtgc 1441 ccggctcatg ctgctgcacc ccagccccaa ctgcagcctc cgagcagaga acctggtcca 1501 cgtccacttc aaagaggaga ttggcattgc tgagctcatc ccgctcgtga ccacctacat 1561 catcctgttt gcctacatct acttctccac acgcaagatc gacatggtca agtccaagtg 1621 gggcctcgcc ctggcagccg tggtcacagt acttagctca ctgctcatgt ctgtggggct 1681 ctgcaccctc ttcggcctga cgcccacact caatggcggt gagatcttcc catacctggt 1741 ggtcgttatt gggctagaga acgtgttggt gctcaccaag tcagtggtat caactccagt 1801 ggacctcgag gtgaagcttc ggattgcaca aggcttgagt agtgagagct ggtccatcat 1861 gaagaacgcg gcgaccgagc tgggcatcat cctcattggc tacttcaccc tcgtgcctgc 1921 tatccaggag ttctgcctct ttgctgttgt gggcctggtg tctgacttct tcctccagat 1981 gctgttcttc accactgtcc tgtcgatcga cattcgccgg atggagctag cagacctaaa 2041 caagcggctg ccccctgaat cctgcctgcc ctcagccaag cccgtgggga ggccagcacg 2101 atatgagaga cagcaggctg tacggccatc cacgccacac accatcacat tgcaaccatc 2161 ttccttccga aacctgcggc ttcccaaaag gctgcgtgtc atctacttcc tggcccgcac 2221 tcgcctggcc cagcgcctca tcatggctgg taccgttgtc tggattggca tcctggtata 2281 caccgacccg gcagggctgc gcacctacct tgctgcccag gtgacagagc agagcccact 2341 gggtgagggt tccctgggcc ccatgcctgt gcctagcgga gtgctgcctg ccagccaccc 2401 ggaccctgca ttctccatct tcccacctga tgctcctaaa ctgccagaga accagacctt 2461 gccaggtgag ctgcctgagc atgctggtcc agcagagggt gtccatgaca gccgagcccc 2521 agaggtaact tgggggcctg aggatgagga gctgtggagg aaattgtcct tccgccactg 2581 gcccacactc ttcaactact acaacatcac actggccaaa aggtacatca gcctgctgcc 2641 tgtcatccct gtcacactac acctgaatcc acgggaggct ctggaggggc gacaccctca 2701 ggatggtcgc agtgcctggg ccccacaaga gcctttgccc gctggcctct gggagtccgg 2761 acctaaggga ccaggtggaa cacagaccca tggcgacatt accttgtaca aggtggccgc 2821 gcttggccta gcagcgggca tcgtcctggt gctgctgctg ctctgcctct accgggtgct 2881 ctgcccgcgt aattatgggc agccgggtgg tggccccggc aggcggaggc gcggggagct 2941 gccctgcgat gactacggct acgcaccgcc cgagacggag atagtgccgc tggtgctgcg 3001 aggtcacctc atggacatcg agtgtctggc tagcgatggg atgctactag tgagctgctg 3061 cctggcaggc caagtctgcg tgtgggacgc tcagacaggg gactgcctca cacggatccc 3121 acgcccaggg ccacgccggg atagctgcgg aggtggagct tttgagactc aggagaactg 3181 ggaaaggctg tcagatggag gcaaggctag cccggaagaa cctggagaca gccctccgct 3241 gcgacgacgc ccccgagggc ctccaccgcc ttccctcttt ggggaccagc ccgacctcac 3301 ctgcttaatc gacaccaact tctcggtgca gctgccccca gagcccactc agcccgagcc 3361 tcggcaccgg gtgggctgtg gccgctctag agactcgggt tatgacttca gccgcctggt 3421 gcagcgtgtg taccaggagg aaggcctggc tgctatgcgc atgccggccc tgcgcccacc 3481 ctcccctgga cctcccttgc cccaggcctc tcaagaagag gggactgcac ctgagaaggg 3541 ctcccctccc ctggcctgga cccccagcac agccggttcc atctggagct tagagctgca 3601 aggcaatctc atcgtggttg ggcggagcag cggccggctg gaggtgtggg acgccattga 3661 gggggtgctc tgctgcagca atgaggagat ctcctcaggc atcacagccc ttgtcttctt 3721 ggacaggagg attgtagctg ctcggcttaa tggttccctt gatttctttt ctttggagac 3781 ccacacttcc ctcagccccc tgcagttcag agggacccca gggcgaggca gttctccttc 3841 ctcatctgtg tacagcagca gcaacacagt gacctgtcat cggacccaca cagtgccctg 3901 tgcacaccag aagcccatca cagccctgag agctgctgcc gggcgcctag tgacagggag 3961 ccaagaccat actctaagag tcttccgact ggatgactcg tgttgcctct ttaccctgaa 4021 gggccactca ggggcaatca cagctgtgta cattgatcag accatggtac tggccagtgg 4081 aggacaagat ggagccatct gcctgtggga tgtactaaca ggcagccggg tcagccaaac 4141 atttgctcac cgtggagatg ttacctccct cacctgtacc gcttcctgtg tcattagtag 4201 tggcctggat gacttcatca gtatctggga ccgcagcaca ggcatcaagc tgtactccat 4261 tcagcaggac ctgggctgtg gtgcaagctt gggtgtcatc tcagataacc ttctggtgac 4321 cggcggccag ggctgtgtct ccttttggga cctaaactat ggggacctgt tacagacagt 4381 ctacttgggc aagaacagtg aagcccagcc tgcccggcag attttggtgt tggacaatgc 4441 tgccattgtc tgcaactttg gcagtgagct cagcctagtg tatgtgccct ctgtgctgga 4501 gaaactggac ctcgaggaac aaaaactaat atcagaggaa gatttggaac agaaactaat 4561 cagtgaagaa gatttagagc aaaagttaat ttccgaggag gacttatagt gagggcccga 4621 tatctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 4681 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 4741 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 4801 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 4861 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 4921 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 4981 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca 5041 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 5101 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 5161 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct 5221 ggaaacgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta 5281 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc 5341 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa 5401 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg 5461 ctcgcagcca acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct 5521 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 5581 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 5641 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta 5701 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 5761 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 5821 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 5881 tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt 5941 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt 6001 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 6061 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 6121 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 6181 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 6241 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 6301 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag 6361 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 6421 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 6481 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 6541 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 6601 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 6661 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 6721 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 6781 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 6841 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc 6901 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 6961 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 7021 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 7081 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 7141 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa 7201 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg 7261 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 7321 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc 7381 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 7441 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg 7501 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 7561 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 7621 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 7681 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 7741 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 7801 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 7861 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 7921 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 7981 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 8041 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 8101 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 8161 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 8221 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 8281 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 8341 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 8401 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 8461 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 8521 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 8581 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 8641 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 8701 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 8761 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 8821 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 8881 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 8941 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 9001 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 9061 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 9121 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 9181 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 9241 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 9301 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 9361 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 9421 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 9481 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 9541 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 9601 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 9661 cctgacgtcg acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct 9721 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg 9781 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga 9841 agaatctgct tagg //