LOCUS       V023703                 7830 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023703
VERSION     V023703
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2556
                     /label="SYVN1(NM_172230)"
                     /note="SYVN1(NM_172230)"
                     /gene="SYVN1"
     CDS             2563..2586
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHHHH"
                     /label="8xHis"
     misc_feature    2606..3194
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3260..3308
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3510..3938
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3952..4281
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4348..5142
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5318..5439
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5488..5504)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5512..5528
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5536..5566)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5581..5602
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5890..6478)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6649..7509)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7510..7614)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgtt ccgcacggca
      721 gtgatgatgg cggccagcct ggcgctgacc ggggctgtgg tggctcacgc ctactacctc
      781 aaacaccagt tctaccccac tgtggtgtac ctgaccaagt ccagccccag catggcagtc
      841 ctgtacatcc aggcctttgt ccttgtcttc cttctgggca aggtgatggg caaggtgttc
      901 tttgggcaac tgagggcagc agagatggag caccttctgg aacgttcctg gtacgccgtc
      961 acagagactt gtctggcctt caccgttttt cgggatgact tcagcccccg ctttgttgca
     1021 ctcttcactc ttcttctctt cctcaaatgt ttccactggc tggctgagga ccgtgtggac
     1081 tttatggaac gcagccccaa catctcctgg ctctttcact gccgcattgt ctctcttatg
     1141 ttcctcctgg gcatcctgga cttcctcttc gtcagccacg cctatcacag catcctgacc
     1201 cgtggggcct ctgtgcagct ggtgtttggc tttgagtatg ccatcctgat gacgatggtg
     1261 ctcaccatct tcatcaagta tgtgctgcac tccgtggacc tccagagtga gaacccctgg
     1321 gacaacaagg ctgtgtacat gctctacaca gagctgttta caggcttcat caaggttctg
     1381 ctgtacatgg ccttcatgac catcatgatc aaggtgcaca ccttcccact ctttgccatc
     1441 cggcccatgt acctggccat gagacagttc aagaaagctg tgacagatgc catcatgtct
     1501 cgccgagcca tccgcaacat gaacaccctg tatccagatg ccaccccaga ggagctccag
     1561 gcaatggaca atgtctgcat catctgccga gaagagatgg tgactggtgc caagagactg
     1621 ccctgcaacc acattttcca taccagctgc ctgcgctcct ggttccagcg gcagcagacc
     1681 tgccccacct gccgtatgga tgtccttcgt gcatcgctgc cagcgcagtc accaccaccc
     1741 ccggagcctg cggatcaggg gccaccccct gccccccacc ccccaccact cttgcctcag
     1801 ccccccaact tcccccaggg cctcctgcct ccttttcctc caggcatgtt cccactgtgg
     1861 ccccccatgg gcccctttcc acctgtcccg cctcccccca gctcaggaga ggctgtggct
     1921 cctccatcca ccagtgcagc agccctttct cggcccagtg gagcagctac aaccacagct
     1981 gctggcacca gtgctactgc tgcttctgcc acagcatctg gcccaggctc tggctctgcc
     2041 ccagaggctg gccctgcccc tggtttcccc ttccctcctc cctggatggg tatgcccctg
     2101 cctccaccct ttgccttccc cccaatgcct gtgccccctg cgggctttgc tgggctgacc
     2161 ccagaggagc tacgagctct ggagggccat gagcggcagc acctggaggc ccggctgcag
     2221 agcctgcgta acatccacac actgctggac gccgccatgc tgcagatcaa ccagtacctc
     2281 accgtgctgg cctccttggg gcccccccgg cctgccactt cagtcaactc cactgaggag
     2341 actgccacta cagttgttgc tgctgcctcc tccaccagca tccctagctc agaggccacg
     2401 accccaaccc caggagcctc cccaccagcc cctgaaatgg aaaggcctcc agctcctgag
     2461 tcagtgggca cagaggagat gcctgaggat ggagagcccg atgcagcaga gctccgccgg
     2521 cgccgcctgc agaagctgga gtctcctgtt gcccacctcg agcaccatca ccatcaccat
     2581 caccattagg gcccgatatc tcgacaatca acctctggat tacaaaattt gtgaaagatt
     2641 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     2701 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     2761 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     2821 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     2881 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     2941 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     3001 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     3061 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     3121 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3181 ggccgcctcc ccgcctggaa acgggggagg ctaactgaaa cacggaagga gacaataccg
     3241 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc
     3301 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     3361 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     3421 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcagatct
     3481 gcgcagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     3541 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3601 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3661 tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3721 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3781 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     3841 ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt
     3901 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     3961 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4021 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4081 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     4141 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     4201 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     4261 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     4321 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     4381 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     4441 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     4501 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     4561 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     4621 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     4681 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     4741 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     4801 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     4861 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     4921 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     4981 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     5041 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     5101 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     5161 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     5221 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     5281 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     5341 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5401 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     5461 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5521 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5581 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5641 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5701 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5761 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5821 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5881 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5941 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6001 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6061 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
     6121 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6181 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6241 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6301 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     6361 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6421 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6481 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6541 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6601 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6661 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6721 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6781 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6841 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6901 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6961 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7021 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7081 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7141 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7201 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7261 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7321 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7381 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7441 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7501 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7561 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7621 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtcg
     7681 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     7741 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     7801 accgacaatt gcatgaagaa tctgcttagg
//
