LOCUS       V028525                10285 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028525
VERSION     V028525
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..4944
                     /label="SNED1(NM_001080437)"
                     /note="SNED1(NM_001080437)"
                     /gene="SNED1"
     CDS             4951..4977
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4981..5007
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5011..5037
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    5061..5649
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    5715..5763
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5965..6393
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6407..6736
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6803..7597
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7773..7894
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7943..7959)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7967..7983
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7991..8021)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8036..8057
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8345..8933)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9104..9964)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9965..10069)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgcg gcacggcgtc
      721 gcctgggcgc tgctggtggc cgcggccctg gggcttgggg cgcgcggggt gcgcggcgcg
      781 gtggcccttg ccgacttcta cccgttcggc gccgagcgcg gcgacgccgt cacccccaag
      841 caggacgacg gcggctcggg gctgcggccg ctctcggtgc ccttcccgtt cttcggtgcc
      901 gagcactccg gactctacgt gaacaacaac gggatcatct ccttcctgaa ggaggtttct
      961 cagttcaccc cagtggcctt ccccattgcc aaggaccgct gcgtggtggc agccttctgg
     1021 gcagatgtgg acaaccggcg tgcaggcgac gtgtactacc gggaggccac cgacccagcc
     1081 atgctgcgcc gagccacgga ggacgtcagg cactacttcc ccgagctcct ggacttcaat
     1141 gccacctggg tttttgttgc cacctggtac cgagtgacct tctttggagg cagttcctca
     1201 tcccctgtca acacattcca gactgtgctc atcacagacg gcaagctctc cttcaccatc
     1261 ttcaactatg agtccatcgt gtggaccaca ggcacacacg ccagcagcgg gggcaacgcc
     1321 actggcctcg ggggcatcgc agcccaggct ggcttcaacg caggcgatgg gcagcgttac
     1381 ttcagtatcc ccggctcgcg cacagcagac atggccgagg tggagaccac caccaacgtg
     1441 ggtgtgcccg ggcgctgggc gttcagaatc gatgatgccc aggtgcgcgt ggggggctgc
     1501 ggccatacaa cgtccgtgtg cctggccctg cgcccctgcc tcaacggcgg caagtgcatc
     1561 gacgactgcg tcacgggcaa cccctcctac acctgctcct gcctctcggg cttcacgggg
     1621 cggaggtgcc acctggacgt gaacgaatgt gcctcccagc cctgtcagaa tggtgggacc
     1681 tgtactcacg gcatcaacag tttccgctgc cagtgcccgg ctggctttgg gggacccacc
     1741 tgtgagacag cccaatcccc ctgtgacacc aaagagtgtc aacatggtgg ccagtgccag
     1801 gtggagaatg gctctgcggt gtgtgtgtgc caggccggat acaccggagc agcctgcgag
     1861 atggatgtgg acgactgcag ccctgacccc tgcctgaatg gaggctcttg tgttgaccta
     1921 gtggggaatt acacctgctt gtgtgccgag cccttcaagg gacttcgctg tgagacagga
     1981 gaccatccag tgccagacgc ctgcctctcg gccccttgcc acaatggggg cacctgtgtg
     2041 gatgcggacc agggctacgt gtgcgagtgc cccgaaggct tcatgggcct ggactgcagg
     2101 gagagagtcc ccgatgactg tgagtgccgc aacggaggca gatgcctggg cgccaacacc
     2161 accctctgcc agtgccccct gggattcttt gggcttctct gtgaatttga aatcacagcc
     2221 atgccctgca acatgaacac acagtgccca gatgggggct actgcatgga gcacggcggg
     2281 agctacctct gcgtctgcca caccgaccac aatgccagcc actccctgcc atcaccctgc
     2341 gactcggacc cctgcttcaa cggaggctcc tgcgatgccc atgacgactc ctacacctgc
     2401 gagtgcccgc gcgggttcca cggcaagcac tgcgagaaag cccggccaca cctgtgcagc
     2461 tcagggccct gccggaacgg gggcacgtgc aaggaggcgg gcggcgagta ccactgcagc
     2521 tgcccctacc gcttcactgg gaggcactgt gagatcggga agccagactc gtgtgcctct
     2581 ggcccctgtc acaacggcgg cacctgcttc cactacattg gcaaatacaa gtgtgactgt
     2641 cccccaggct tctccgggcg gcactgcgag atagccccct ccccctgctt ccggagcccg
     2701 tgtgtgaatg ggggcacctg cgaggaccgg gacacggatt tcttctgcca ctgccaagca
     2761 gggtacatgg gacgccggtg ccaggcagag gtggactgcg gccccccgga ggaggtgaag
     2821 cacgccacac tgcgcttcaa cggcacgcgg ctgggcgcgg tggccctgta tgcatgtgac
     2881 cgtggctaca gcctgagcgc ccccagccgc atccgggtct gccagccaca cggtgtctgg
     2941 agtgagcctc cccagtgcct tgaaatcgat gagtgccggt ctcagccgtg cctgcatggg
     3001 ggctcttgtc aggaccgcgt tgctgggtac ctgtgcctct gcagcacagg ctatgagggc
     3061 gcccactgtg agctggagag ggatgagtgc cgagctcacc cgtgcagaaa tggagggtcc
     3121 tgcaggaacc tcccaggggc ctatgtctgc cggtgccctg caggcttcgt tggagtccac
     3181 tgtgagacag aggtggacgc ctgcgactcc agcccctgcc agcatggagg ccggtgtgag
     3241 agcggcggcg gggcctacct gtgcgtctgc ccagagagct tcttcggcta ccactgcgag
     3301 acagtgagtg acccctgctt ctccagcccc tgtgggggcc gtggctattg cctggccagc
     3361 aacggctccc acagctgcac ctgcaaagtg ggctacacgg gcgaggactg cgccaaagag
     3421 ctcttcccac cgacggccct caagatggag agagtggagg agagtggggt ctctatctcc
     3481 tggaacccgc ccaatggtcc agccgccagg cagatgcttg atggctacgc ggtcacctac
     3541 gtctcctccg acggctccta ccgccgcaca gactttgtgg acaggacccg ctcctcgcac
     3601 cagctccagg ccctggcggc cggcagggcc tacaacatct ccgtcttctc agtgaagcga
     3661 aacagtaaca acaagaatga catcagcagg cctgccgtgc tgctggcccg cacgcgaccc
     3721 cgccctgtgg aaggcttcga ggtcaccaat gtgacggcta gcaccatctc agtgcagtgg
     3781 gccctgcaca ggatccgcca tgccaccgtc agtggggtcc gtgtgtccat ccgccaccct
     3841 gaggccctca gggaccaggc caccgatgtg gacaggagtg tggacaggtt cacctttagg
     3901 gccctgctgc ctgggaagag gtacaccatc cagctgacca ccctcagtgg gctcagggga
     3961 gaggagcacc ccacagagag cctggccacc gcgccgacgc acgtgtggac ccggcccctg
     4021 cctccagcaa acctgaccgc cgcccgagtc actgccacct ctgcccacgt ggtctgggat
     4081 gccccgactc caggcagctt gctggaggct tatgtcatca atgtgaccac cagccagagc
     4141 accaagagcc gctatgtccc caacgggaag ctggcgtcct acacggtgcg cgacctgctg
     4201 ccgggacggc ggtaccagct ctctgtgata gcagtgcaga gcacggagct cgggccgcag
     4261 cacagcgagc ccgcccacct ctacatcatc acctccccca gggatggcgc tgacagacgc
     4321 tggcaccagg gaggacacca ccctcgggtg ctcaagaaca gaccgccccc ggcgcgcctg
     4381 ccggagctgc gcctgctcaa tgaccacagc gcccccgaga cccccaccca gccccccagg
     4441 ttctcggagc ttgtggacgg cagaggaaga gtgagcgcca ggttcggtgg ctcacccagc
     4501 aaagcagcca ccgtgagatc acaacccaca gcctcggcgc agctcgagaa catggaggaa
     4561 gcccccaagc gggtcagcct ggccctccag ctccctgaac acggcagcaa ggacatcgga
     4621 aacgtccctg gcaactgttc agaaaacccc tgtcagaacg gaggcacttg tgtgccgggc
     4681 gcagacgccc acagctgtga ctgcgggcca gggttcaaag gcagacgctg cgagctcgcc
     4741 tgtataaagg tgtcccgccc ctgcacaagg ctgttctccg agacaaaggc ctttccagtc
     4801 tgggagggag gcgtctgtca ccacgtgtat aaaagagtct accgagttca ccaagacatc
     4861 tgcttcaaag agagctgtga aagcacaagc ctcaagaaga ccccaaacag gaaacaaagt
     4921 aagagtcaga cactggagaa atctctcgag tacccatacg acgtacctga ttatgcaact
     4981 tatccttatg atgtacctga ttatgctagc tatccttatg atgtcccaga ctacgcatag
     5041 tgagggcccg atatctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     5101 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5161 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5221 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5281 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5341 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5401 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     5461 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5521 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5581 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5641 cctccccgcc tggaaacggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     5701 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg
     5761 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
     5821 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
     5881 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagca gatctgcgca
     5941 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6001 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6061 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6121 gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6181 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6241 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6301 tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa
     6361 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     6421 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6481 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6541 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     6601 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6661 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6721 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     6781 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     6841 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     6901 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     6961 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     7021 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     7081 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     7141 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     7201 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     7261 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     7321 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     7381 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     7441 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     7501 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     7561 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa
     7621 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     7681 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     7741 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     7801 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7861 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     7921 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7981 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     8041 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     8101 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     8161 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8221 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8281 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8341 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8401 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8461 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8521 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     8581 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8641 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8701 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8761 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     8821 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8881 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8941 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9001 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9061 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9121 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9181 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9241 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9301 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9361 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9421 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9481 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9541 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9601 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9661 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9721 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9781 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9841 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9901 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9961 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10021 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10081 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtcgactct
    10141 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
    10201 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
    10261 caattgcatg aagaatctgc ttagg
//
