LOCUS pEnCMV-SMS(human)-HA 7144 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V040069 VERSION V040069 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..1803 /label="SMS(NM_004595)" /note="SMS(NM_004595)" /gene="SMS" CDS 1810..1836 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1840..1866 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1870..1896 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 1920..2508 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2574..2622 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 2824..3252 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3266..3595 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3662..4456 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4632..4753 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4802..4818) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4826..4842 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4850..4880) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4895..4916 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5204..5792) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5963..6823) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6824..6928) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc agcagcacgg 721 cacagcacgc tcgacttcat gctcggcgcc aaagctgatg gtgagaccat tctaaaaggc 781 ctccagtcca ttttccagga gcaggggatg gcggagtcgg tgcacacctg gcaggaccat 841 ggctatttag caacctacac aaacaagaac ggcagctttg ccaatttgag aatttaccca 901 catggattgg tgttgctgga ccttcagagt tatgatggtg atgcgcaagg caaagaagag 961 atcgacagta ttttgaacaa agtagaggaa agaatgaaag aattgagtca ggacagtact 1021 gggcgggtga aacgattacc acccatagtg cgaggaggag ccatcgacag atactggccc 1081 accgccgacg ggcgcctggt tgaatatgac atagatgaag tggtatatga cgaagattca 1141 ccttatcaaa atataaaaat tctacactcg aagcagtttg gaaatattct catccttagt 1201 ggggatgtta atttggcaga gagtgatttg gcatataccc gggccatcat gggcagtggc 1261 aaagaagatt acactggcaa agatgtactc attctgggag gtggagacgg aggcatattg 1321 tgtgaaatag tcaaactaaa accaaagatg gtcactatgg tagagattga ccaaatggtg 1381 attgatgggt gtaagaaata catgcgaaaa acgtgtggcg atgtcttaga caatcttaaa 1441 ggagactgct atcaggttct aatagaagac tgtatcccgg tactgaagag gtacgccaaa 1501 gaagggagag aatttgatta tgtgattaat gatttgacag ctgttccaat ctccacgtct 1561 ccagaagaag attccacatg ggagtttctc agactgattc ttgacctctc aatgaaagtg 1621 ttgaaacagg atgggaaata ttttacacag gggaactgtg tcaatctgac agaagcactg 1681 tcgctctatg aagaacagct ggggcgcctg tattgtcctg tggaattttc aaaggagatc 1741 gtctgtgtcc cttcatactt ggaattgtgg gtattttaca ctgtttggaa gaaagctaaa 1801 cccctcgagt acccatacga cgtacctgat tatgcaactt atccttatga tgtacctgat 1861 tatgctagct atccttatga tgtcccagac tacgcatagt gagggcccga tatctcgaca 1921 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 1981 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 2041 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 2101 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 2161 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 2221 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 2281 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg 2341 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 2401 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 2461 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaaacgggg 2521 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat 2581 aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt 2641 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg 2701 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca 2761 acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct agggggtatc 2821 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 2881 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 2941 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat 3001 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 3061 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 3121 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 3181 tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 3241 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc 3301 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 3361 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 3421 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 3481 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 3541 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 3601 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg 3661 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 3721 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 3781 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 3841 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 3901 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 3961 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 4021 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 4081 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 4141 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 4201 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 4261 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 4321 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 4381 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 4441 tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca 4501 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 4561 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 4621 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 4681 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 4741 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat 4801 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 4861 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 4921 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 4981 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 5041 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 5101 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 5161 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 5221 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 5281 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 5341 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat 5401 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 5461 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 5521 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 5581 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 5641 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 5701 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 5761 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 5821 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 5881 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 5941 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 6001 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 6061 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 6121 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 6181 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 6241 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 6301 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 6361 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 6421 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 6481 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 6541 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 6601 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 6661 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 6721 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 6781 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 6841 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 6901 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg 6961 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc 7021 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc 7081 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct 7141 tagg //