LOCUS       V037937                 8896 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037937
VERSION     V037937
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..3555
                     /label="SMG5(NM_001323617)"
                     /note="SMG5(NM_001323617)"
                     /gene="SMG5"
     CDS             3562..3588
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3592..3618
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3622..3648
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    3672..4260
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4326..4374
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4576..5004
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5018..5347
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5414..6208
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6384..6505
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6554..6570)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6578..6594
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6602..6632)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6647..6668
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6956..7544)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7715..8575)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8576..8680)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgtt cctgcaccca
      721 gtggactatg ggagaaaggc tgaggagctg ctgtggagaa aggtatacta tgaagttatc
      781 cagcttatca agactaacaa aaagcacatc cacagccgga gcactttgga atgtgcctac
      841 aggacgcacc tggttgctgg tattggcttc taccagcatc tccttctcta tatccagtcc
      901 cactaccagc tggaactgca gtgctgcatc gactggaccc atgtcactga ccccctcata
      961 ggatgcaaga agccagtgtc tgcctcaggg aaggagatgg attgggcaca gatggcatgt
     1021 caccgatgtc tggtgtatct gggggatttg tcccgatatc agaatgaatt agctggcgta
     1081 gataccgagc tgctagccga gagattttac taccaagccc tgtcagtagc tcctcagatt
     1141 ggaatgccct tcaatcagct gggcaccctg gcaggcagca agtactataa tgtggaagcc
     1201 atgtattgct acctgcgctg catccagtca gaagtgtcct ttgagggagc ctatgggaac
     1261 ctcaagcggc tgtatgacaa ggcagccaaa atgtaccacc aactgaagaa gtgtgagact
     1321 cggaaactgt ctcctggcaa aaagcgatgt aaagacatta aaaggttgct agtgaacttt
     1381 atgtatctgc aaagcctcct acagcccaaa agcagctccg tggactcaga gctgacctca
     1441 ctttgccagt cagtcctgga ggacttcaac ctctgcctct tctacctgcc ctcctcaccc
     1501 aacctcagcc tggccagtga ggatgaggag gagtatgaga gtggatatgc tttcctcccg
     1561 gaccttctca tctttcaaat ggtcatcatc tgccttatgt gtgtgcacag cttggagaga
     1621 gcaggatcca agcagtacag tgcagccatt gccttcaccc tggccctctt ttcccacctc
     1681 gtcaatcatg tcaacatacg gctgcaggct gagctggaag agggcgagaa tcccgtcccg
     1741 gcattccaga gtgatggcac agatgaacca gagtccaagg aacctgtgga gaaagaggag
     1801 gagccagatc ctgagcctcc tcctgtaaca ccccaagtgg gtgagggcag aaagagccgt
     1861 aagttctctc gcctctcctg tctccgccgt cgccgccacc cacccaaagt tggtgatgac
     1921 agtgacctga gtgaaggctt tgaatcggac tcaagccatg actcagcccg ggccagtgag
     1981 ggctcagaca gtggctctga caagagtctt gaaggtgggg gaacggcctt tgatgctgaa
     2041 acagactcgg aaatgaatag ccaggagtcc cgatcagact tggaagatat ggaggaagag
     2101 gaggggacac ggtcaccaac cctggagccc cctcggggca gatcagaggc tcccgattcc
     2161 ctcaatggcc cactgggccc cagtgaggct agcattgcca gcaatctaca agccatgtcc
     2221 acccagatgt tccagactaa gcgctgcttc cgactggccc ccacctttag caacctgctc
     2281 ctccagccca ccaccaaccc tcatacctcg gccagccaca ggccttgcgt caatggggat
     2341 gtagacaagc cttcagagcc agcctctgag gagggctctg agtcggaggg gagtgagtcc
     2401 agtggacgct cctgtcggaa tgagcgcagc atccaggaga agcttcaggt cctgatggcc
     2461 gaaggtctgc ttcctgctgt gaaagtcttc ctggactggc ttcggaccaa ccccgacctc
     2521 atcatcgtgt gtgcgcagag ctctcaaagt ctgtggaacc gcctgtctgt gttgctgaat
     2581 ctgttgcctg ctgctggtga actccaggag tctggcctgg ccttgtgtcc tgaggtccaa
     2641 gatcttcttg aaggttgtga actgcctgac ctcccctcta gccttctgct cccagaggac
     2701 atggctcttc gtaacctgcc cccgctccga gctgcccaca gacgctttaa ctttgacacg
     2761 gatcggcccc tgctcagcac cttagaggag tcagtggtgc gcatctgctg catccgcagc
     2821 tttggtcatt tcatcgcccg cctgcaaggc agcatcctgc agttcaaccc agaggttggc
     2881 atcttcgtca gcattgccca gtctgagcag gagagcctgc tgcagcaggc ccaggcacag
     2941 ttccgaatgg cacaggagga agctcgtcgg aacaggctca tgagagacat ggctcagcta
     3001 cgacttcagc tcgaagtgtc tcagctggag ggcagcctgc agcagcccaa ggcccagtca
     3061 gccatgtctc cctacctcgt ccctgacacc caggccctct gccaccatct ccctgtcatc
     3121 cgccaactgg ccaccagtgg ccgcttcatt gtcatcatcc caaggacagt gatcgatggc
     3181 ctggatttgc tgaagaagga acacccaggg gcccgggatg ggattcggta cctggaggca
     3241 gagtttaaaa aaggaaacag gtacattcgc tgccagaaag aggtgggaaa gagctttgag
     3301 cggcataagc tgaagaggca ggatgcagat gcctggactc tctataagat cctagacagc
     3361 tgcaaacagc tgactctggc ccagggggca ggtgaggagg atccgagtgg catggtgacc
     3421 atcatcacag gccttccact ggacaacccc agcgtgcttt caggccccat gcaggcagcc
     3481 ctgcaggccg ctgcccacgc cagtgtggac atcaagaatg ttctggactt ctacaagcag
     3541 tggaaggaaa ttggtctcga gtacccatac gacgtacctg attatgcaac ttatccttat
     3601 gatgtacctg attatgctag ctatccttat gatgtcccag actacgcata gtgagggccc
     3661 gatatctcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     3721 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     3781 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     3841 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     3901 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     3961 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4021 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     4081 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     4141 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     4201 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     4261 ctggaaacgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     4321 tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac
     4381 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     4441 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     4501 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc agatctgcgc agctggggct
     4561 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4621 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4681 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt
     4741 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     4801 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4861 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     4921 attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga
     4981 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     5041 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5101 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5161 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5221 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5281 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5341 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     5401 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     5461 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     5521 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     5581 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     5641 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     5701 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     5761 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     5821 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     5881 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     5941 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     6001 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     6061 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     6121 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     6181 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgcgaa atgaccgacc
     6241 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     6301 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     6361 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     6421 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6481 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     6541 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     6601 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     6661 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     6721 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     6781 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6841 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6901 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6961 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7021 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7081 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7141 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7201 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7261 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7321 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7381 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7441 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7501 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7561 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7621 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7681 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7741 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7801 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7861 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7921 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7981 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8041 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8101 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8161 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8221 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8281 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8341 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8401 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8461 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8521 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8581 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8641 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8701 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat
     8761 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     8821 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     8881 gaagaatctg cttagg
//
