LOCUS       V024243                 9520 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024243
VERSION     V024243
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..1422
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1474..4176
                     /label="SLFN11(NM_001104587)"
                     /note="SLFN11(NM_001104587)"
                     /gene="SLFN11"
     CDS             4183..4272
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    4296..4884
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4950..4998
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5200..5628
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5642..5971
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6038..6832
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7008..7129
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7178..7194)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7202..7218
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7226..7256)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7271..7292
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7580..8168)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8339..9199)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9200..9304)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggt gagcaagggc
      721 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      781 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      841 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      901 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      961 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1021 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1081 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1141 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1201 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1261 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     1321 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1381 accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact cagaggtgga
     1441 ggtggctcag gtggcggagg ttcttgcggc cgcatggagg caaatcagtg ccccctggtt
     1501 gtggaaccat cttacccaga cctggtcatc aatgtaggag aagtgactct tggagaagaa
     1561 aacagaaaaa agctgcagaa aattcagaga gaccaagaga aggagagagt tatgcgggct
     1621 gcatgtgctt tattaaactc aggaggagga gtgattcgaa tggccaagaa ggttgagcat
     1681 cccgtggaga tgggactgga tttagaacag tctttgagag agcttattca gtcttcagat
     1741 ctgcaggctt tctttgagac caagcaacaa ggaaggtgtt tttacatttt tgttaaatct
     1801 tggagcagtg gccctttccc tgaagatcgc tctgtcaagc cccgcctttg cagcctcagt
     1861 tcttcattat accgtagatc tgagacctct gtgcgttcca tggactcaag agaggcattc
     1921 tgtttcctga agaccaaaag gaagccaaaa atcttggaag aaggaccttt tcacaaaatt
     1981 cacaagggtg tataccaaga gctccctaac tcggatcctg ctgacccaaa ctcggatcct
     2041 gctgacctaa ttttccaaaa agactatctt gaatatggtg aaatcctgcc ttttcctgag
     2101 tctcagttag tagagtttaa acagttctct acaaaacact tccaagaata tgtaaaaagg
     2161 acaattccag aatacgtccc tgcatttgca aacactggag gaggctatct ttttattgga
     2221 gtggatgata agagtaggga agtcctggga tgtgcaaaag aaaatgttga ccctgactct
     2281 ttgagaagga aaatagaaca agccatatac aaactacctt gtgttcattt ttgccaaccc
     2341 caacgcccga taaccttcac actcaaaatt gtgaatgtgt taaaaagggg agagctctat
     2401 ggctatgctt gcatgatcag agtaaatccc ttctgctgtg cagtgttctc agaagctccc
     2461 aattcatgga tagtggagga caagtacgtc tgcagcctga caaccgagaa atgggtaggc
     2521 atgatgacag acacagatcc agatcttcta cagttgtctg aagattttga atgtcagctg
     2581 agtctatcta gtgggcctcc ccttagcaga ccagtgtact ccaagaaagg cctggaacat
     2641 aaaaaggaac tccagcaact tttattttca gtcccaccag gatatttgcg atatactcca
     2701 gagtcactct ggagggacct gatctcagag cacagaggac tagaggagtt aataaataag
     2761 caaatgcaac ctttctttcg gggaattttg atcttctcta gaagttgggc tgtggacctg
     2821 aacttgcagg agaagccagg agtcatctgt gatgctctgc tgatagcaca gaacagcacc
     2881 cccattctct acaccattct cagggagcaa gatgcagagg gccaggacta ctgcactcgc
     2941 accgccttta ctttgaagca gaagctagtg aacatggggg gctacaccgg gaaggtgtgt
     3001 gtcagggcca aggtcctctg cctgagtcct gagagcagcg cagaggcctt ggaggctgca
     3061 gtgtctccga tggattaccc tgcgtcctat agccttgcag gcacccagca catggaagcc
     3121 ctgctgcagt ccctcgtgat tgtcttactc ggcttcaggt ctctcttgag tgaccagctc
     3181 ggctgtgagg ttttaaatct gctcacagcc cagcagtatg agatattctc cagaagcctc
     3241 cgcaagaaca gagagttgtt tgtccacggc ttacctggct cagggaagac catcatggcc
     3301 atgaagatca tggagaagat caggaatgtg tttcactgtg aggcacacag aattctctac
     3361 gtttgtgaaa accagcctct gaggaacttt atcagtgata gaaatatctg ccgagcagag
     3421 acccggaaaa ctttcctaag agaaaacttt gaacacattc aacacatcgt cattgacgaa
     3481 gctcagaatt tccgtactga agatggggac tggtatggga aggcaaaaag catcactcgg
     3541 agagcaaagg gtggcccagg aattctctgg atctttctgg attactttca gaccagccac
     3601 ttggattgca gtggcctccc tcctctctca gaccaatatc caagagaaga gctcaccaga
     3661 atagttcgca atgcagatcc aatagccaag tacttacaaa aagaaatgca agtaattaga
     3721 agtaatcctt catttaacat ccccactggg tgcctcgagg tatttcctga agccgaatgg
     3781 tcccagggtg ttcagggaac cttacgaatt aagaaatact tgactgtgga gcaaataatg
     3841 acctgtgtgg cagacacgtg caggcgcttc tttgataggg gctattctcc aaaggatgtt
     3901 gctgtgcttg tcagcaccgc aaaagaagtg gagcactata agtatgagct cttgaaagca
     3961 atgaggaaga aaagggtggt gcagctcagt gatgcatgtg atatgttggg tgatcacatt
     4021 gtgttggaca gtgttcggcg attctcaggc ctggaaagga gcatagtgtt tgggatccat
     4081 ccaaggacag ctgacccagc tatcttaccc aatgttctga tctgtctggc ttccagggca
     4141 aaacaacacc tgtatatttt tccgtggggt ggccatctcg aggaacaaaa actaatatca
     4201 gaggaagatt tggaacagaa actaatcagt gaagaagatt tagagcaaaa gttaatttcc
     4261 gaggaggact tatagtgagg gcccgatatc tcgacaatca acctctggat tacaaaattt
     4321 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4381 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4441 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4501 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4561 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4621 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4681 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     4741 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4801 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4861 tctccctttg ggccgcctcc ccgcctggaa acgggggagg ctaactgaaa cacggaagga
     4921 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     4981 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     5041 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     5101 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     5161 tagcagatct gcgcagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     5221 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5281 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5341 aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5401 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     5461 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     5521 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg
     5581 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     5641 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5701 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     5761 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     5821 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5881 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     5941 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     6001 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     6061 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     6121 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     6181 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     6241 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     6301 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     6361 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     6421 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     6481 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     6541 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     6601 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     6661 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     6721 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     6781 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     6841 ctggggttcg cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     6901 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     6961 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     7021 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7081 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     7141 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7201 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7261 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7321 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7381 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7441 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7501 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7561 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7621 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7681 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7741 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     7801 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7861 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7921 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7981 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     8041 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8101 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8161 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8221 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8281 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8341 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8401 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8461 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8521 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8581 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8641 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8701 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8761 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8821 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8881 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8941 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9001 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9061 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9121 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9181 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9241 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9301 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     9361 cctatggtcg actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     9421 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     9481 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
//
