LOCUS       V018453                11942 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018453
VERSION     V018453
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             665..6613
                     /label="SCN5A(NM_001160160)"
                     /note="SCN5A(NM_001160160)"
                     /gene="SCN5A"
     CDS             6629..6694
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    6718..7306
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7372..7420
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7622..8050
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8064..8393
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8460..9254
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9430..9551
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9600..9616)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9624..9640
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9648..9678)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9693..9714
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10002..10590)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10761..11621)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11622..11726)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgc
      661 caccatggca aacttcctat tacctcgggg caccagcagc ttccgcaggt tcacacggga
      721 gtccctggca gccatcgaga agcgcatggc agagaagcaa gcccgcggct caaccacctt
      781 gcaggagagc cgagaggggc tgcccgagga ggaggctccc cggccccagc tggacctgca
      841 ggcctccaaa aagctgccag atctctatgg caatccaccc caagagctca tcggagagcc
      901 cctggaggac ctggacccct tctatagcac ccaaaagact ttcatcgtac tgaataaagg
      961 caagaccatc ttccggttca gtgccaccaa cgccttgtat gtcctcagtc ccttccaccc
     1021 catccggaga gcggctgtga agattctggt tcactcgctc ttcaacatgc tcatcatgtg
     1081 caccatcctc accaactgcg tgttcatggc ccagcacgac cctccaccct ggaccaagta
     1141 tgtcgagtac accttcaccg ccatttacac ctttgagtct ctggtcaaga ttctggctcg
     1201 aggcttctgc ctgcacgcgt tcactttcct tcgggaccca tggaactggc tggactttag
     1261 tgtgattatc atggcgtatg tatcagaaaa tataaaacta ggcaatttgt cggctcttcg
     1321 aactttcaga gtcctgagag ctctaaaaac tatttcagtt atcccagggc tgaagaccat
     1381 cgtgggggcc ctgatccagt ctgtgaagaa gctggctgat gtgatggtcc tcacagtctt
     1441 ctgcctcagc gtctttgccc tcatcggcct gcagctcttc atgggcaacc taaggcacaa
     1501 gtgcgtgcgc aacttcacag cgctcaacgg caccaacggc tccgtggagg ccgacggctt
     1561 ggtctgggaa tccctggacc tttacctcag tgatccagaa aattacctgc tcaagaacgg
     1621 cacctctgat gtgttactgt gtgggaacag ctctgacgct gggacatgtc cggagggcta
     1681 ccggtgccta aaggcaggcg agaaccccga ccacggctac accagcttcg attcctttgc
     1741 ctgggccttt cttgcactct tccgcctgat gacgcaggac tgctgggagc gcctctatca
     1801 gcagaccctc aggtccgcag ggaagatcta catgatcttc ttcatgcttg tcatcttcct
     1861 ggggtccttc tacctggtga acctgatcct ggccgtggtc gcaatggcct atgaggagca
     1921 aaaccaagcc accatcgctg agaccgagga gaaggaaaag cgcttccagg aggccatgga
     1981 aatgctcaag aaagaacacg aggccctcac catcaggggt gtggataccg tgtcccgtag
     2041 ctccttggag atgtcccctt tggccccagt aaacagccat gagagaagaa gcaagaggag
     2101 aaaacggatg tcttcaggaa ctgaggagtg tggggaggac aggctcccca agtctgactc
     2161 agaagatggt cccagagcaa tgaatcatct cagcctcacc cgtggcctca gcaggacttc
     2221 tatgaagcca cgttccagcc gcgggagcat tttcaccttt cgcaggcgag acctgggttc
     2281 tgaagcagat tttgcagatg atgaaaacag cacagcgggg gagagcgaga gccaccacac
     2341 atcactgctg gtgccctggc ccctgcgccg gaccagtgcc cagggacagc ccagtcccgg
     2401 aacctcggct cctggccacg ccctccatgg caaaaagaac agcactgtgg actgcaatgg
     2461 ggtggtctca ttactggggg caggcgaccc agaggccaca tccccaggaa gccacctcct
     2521 ccgccctgtg atgctagagc acccgccaga cacgaccacg ccatcggagg agccaggcgg
     2581 gccccagatg ctgacctccc aggctccgtg tgtagatggc ttcgaggagc caggagcacg
     2641 gcagcgggcc ctcagcgcag tcagcgtcct caccagcgca ctggaagagt tagaggagtc
     2701 tcgccacaag tgtccaccat gctggaaccg tctcgcccag cgctacctga tctgggagtg
     2761 ctgcccgctg tggatgtcca tcaagcaggg agtgaagttg gtggtcatgg acccgtttac
     2821 tgacctcacc atcactatgt gcatcgtact caacacactc ttcatggcgc tggagcacta
     2881 caacatgaca agtgaattcg aggagatgct gcaggtcgga aacctggtct tcacagggat
     2941 tttcacagca gagatgacct tcaagatcat tgccctcgac ccctactact acttccaaca
     3001 gggctggaac atcttcgaca gcatcatcgt catccttagc ctcatggagc tgggcctgtc
     3061 ccgcatgagc aacttgtcgg tgctgcgctc cttccgcctg ctgcgggtct tcaagctggc
     3121 caaatcatgg cccaccctga acacactcat caagatcatc gggaactcag tgggggcact
     3181 ggggaacctg acactggtgc tagccatcat cgtgttcatc tttgctgtgg tgggcatgca
     3241 gctctttggc aagaactact cggagctgag ggacagcgac tcaggcctgc tgcctcgctg
     3301 gcacatgatg gacttctttc atgccttcct catcatcttc cgcatcctct gtggagagtg
     3361 gatcgagacc atgtgggact gcatggaggt gtcggggcag tcattatgcc tgctggtctt
     3421 cttgcttgtt atggtcattg gcaaccttgt ggtcctgaat ctcttcctgg ccttgctgct
     3481 cagctccttc agtgcagaca acctcacagc ccctgatgag gacagagaga tgaacaacct
     3541 ccagctggcc ctggcccgca tccagagggg cctgcgcttt gtcaagcgga ccacctggga
     3601 tttctgctgt ggtctcctgc ggcagcggcc tcagaagccc gcagcccttg ccgcccaggg
     3661 ccagctgccc agctgcattg ccacccccta ctccccgcca cccccagaga cggagaaggt
     3721 gcctcccacc cgcaaggaaa cacggtttga ggaaggcgag caaccaggcc agggcacccc
     3781 cggggatcca gagcccgtgt gtgtgcccat cgctgtggcc gagtcagaca cagatgacca
     3841 agaagaagat gaggagaaca gcctgggcac ggaggaggag tccagcaagc aggaatccca
     3901 gcctgtgtcc ggtggcccag aggcccctcc ggattccagg acctggagcc aggtgtcagc
     3961 gactgcctcc tctgaggccg aggccagtgc atctcaggcc gactggcggc agcagtggaa
     4021 agcggaaccc caggccccag ggtgcggtga gaccccagag gacagttgct ccgagggcag
     4081 cacagcagac atgaccaaca ccgctgagct cctggagcag atccctgacc tcggccagga
     4141 tgtcaaggac ccagaggact gcttcactga aggctgtgtc cggcgctgtc cctgctgtgc
     4201 ggtggacacc acacaggccc cagggaaggt ctggtggcgg ttgcgcaaga cctgctacca
     4261 catcgtggag cacagctggt tcgagacatt catcatcttc atgatcctac tcagcagtgg
     4321 agcgctggcc ttcgaggaca tctacctaga ggagcggaag accatcaagg ttctgcttga
     4381 gtatgccgac aagatgttca catatgtctt cgtgctggag atgctgctca agtgggtggc
     4441 ctacggcttc aagaagtact tcaccaatgc ctggtgctgg ctcgacttcc tcatcgtaga
     4501 cgtctctctg gtcagcctgg tggccaacac cctgggcttt gccgagatgg gccccatcaa
     4561 gtcactgcgg acgctgcgtg cactccgtcc tctgagagct ctgtcacgat ttgagggcat
     4621 gagggtggtg gtcaatgccc tggtgggcgc catcccgtcc atcatgaacg tcctcctcgt
     4681 ctgcctcatc ttctggctca tcttcagcat catgggcgtg aacctctttg cggggaagtt
     4741 tgggaggtgc atcaaccaga cagagggaga cttgcctttg aactacacca tcgtgaacaa
     4801 caagagccag tgtgagtcct tgaacttgac cggagaattg tactggacca aggtgaaagt
     4861 caactttgac aacgtggggg ccgggtacct ggcccttctg caggtggcaa catttaaagg
     4921 ctggatggac attatgtatg cagctgtgga ctccaggggg tatgaagagc agcctcagtg
     4981 ggaatacaac ctctacatgt acatctattt tgtcattttc atcatctttg ggtctttctt
     5041 caccctgaac ctctttattg gtgtcatcat tgacaacttc aaccaacaga agaaaaagtt
     5101 agggggccag gacatcttca tgacagagga gcagaagaag tactacaatg ccatgaagaa
     5161 gctgggctcc aagaagcccc agaagcccat cccacggccc ctgaacaagt accagggctt
     5221 catattcgac attgtgacca agcaggcctt tgacgtcacc atcatgtttc tgatctgctt
     5281 gaatatggtg accatgatgg tggagacaga tgaccaaagt cctgagaaaa tcaacatctt
     5341 ggccaagatc aacctgctct ttgtggccat cttcacaggc actgtgctct cggacatcat
     5401 ccagaagtac ttcttctccc cgacgctctt ccgagtcatc cgcctggccc gaataggccg
     5461 catcctcaga ctgatccgag gggccaaggg gatccgcacg ctgctctttg ccctcatgat
     5521 gtccctgcct gccctcttca acatcgggct gctgctcttc ctcgtcatgt tcatctactc
     5581 catctttggc atggccaact tcgcttatgt caagtgggag gctggcatcg acgacatgtt
     5641 caacttccag accttcgcca acagcatgct gtgcctcttc cagatcacca cgtcggccgg
     5701 ctgggatggc ctcctcagcc ccatcctcaa cactgggccg ccctactgcg accccactct
     5761 gcccaacagc aatggctctc ggggggactg cgggagccca gccgtgggca tcctcttctt
     5821 caccacctac atcatcatct ccttcctcat cgtggtcaac atgtacattg ccatcatcct
     5881 ggagaacttc agcgtggcca cggaggagag caccgagccc ctgagtgagg acgacttcga
     5941 tatgttctat gagatctggg agaaatttga cccagaggcc actcagttta ttgagtattc
     6001 ggtcctgtct gactttgccg atgccctgtc tgagccactc cgtatcgcca agcccaacca
     6061 gataagcctc atcaacatgg acctgcccat ggtgagtggg gaccgcatcc attgcatgga
     6121 cattctcttt gccttcacca aaagggtcct gggggagtct ggggagatgg acgccctgaa
     6181 gatccagatg gaggagaagt tcatggcagc caacccatcc aagatctcct acgagcccat
     6241 caccaccaca ctccggcgca agcacgaaga ggtgtcggcc atggttatcc agagagcctt
     6301 ccgcaggcac ctgctgcaac gctctttgaa gcatgcctcc ttcctcttcc gtcagcaggc
     6361 gggcagcggc ctctccgaag aggatgcccc tgagcgagag ggcctcatcg cctacgtgat
     6421 gagtgagaac ttctcccgac cccttggccc accctccagc tcctccatct cctccacttc
     6481 cttcccaccc tcctatgaca gtgtcactag agccaccagc gataacctcc aggtgcgggg
     6541 gtctgactac agccacagtg aagatctcgc cgacttcccc ccttctccgg acagggaccg
     6601 tgagtccatc gtgcagcggc cgctcgagga ctacaaagac catgacggtg attataaaga
     6661 tcatgacatc gactacaagg atgacgatga caagtagtga gggcccgata tctcgacaat
     6721 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     6781 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     6841 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     6901 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6961 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7021 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7081 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     7141 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7201 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     7261 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aaacggggga
     7321 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     7381 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     7441 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     7501 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     7561 gtcggggcgg caggccctgc catagcagat ctgcgcagct ggggctctag ggggtatccc
     7621 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7681 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7741 acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt
     7801 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7861 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7921 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7981 taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8041 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     8101 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     8161 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8221 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     8281 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     8341 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     8401 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
     8461 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     8521 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     8581 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     8641 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     8701 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     8761 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     8821 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     8881 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     8941 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     9001 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     9061 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     9121 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     9181 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     9241 acgagttctt ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac
     9301 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     9361 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     9421 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9481 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9541 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     9601 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9661 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9721 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9781 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9841 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9901 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9961 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    10021 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    10081 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    10141 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
    10201 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    10261 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    10321 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    10381 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10441 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10501 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10561 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10621 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10681 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10741 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10801 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10861 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10921 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10981 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    11041 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    11101 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    11161 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    11221 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    11281 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    11341 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    11401 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    11461 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    11521 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    11581 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11641 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11701 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
    11761 ggatcgggag atctcccgat cccctatggt cgactctcag tacaatctgc tctgatgccg
    11821 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
    11881 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
    11941 gg
//
