LOCUS       V025056                12098 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025056
VERSION     V025056
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             779..6778
                     /label="SCN3A(NM_006922)"
                     /note="SCN3A(NM_006922)"
                     /gene="SCN3A"
     CDS             6785..6850
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    6874..7462
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7528..7576
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7778..8206
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8220..8549
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8616..9410
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9586..9707
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9756..9772)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9780..9796
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9804..9834)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9849..9870
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10158..10746)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10917..11777)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11778..11882)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcggatcca ctagtccagt gtggtggaat tcgccaccat
      781 ggcacaggca ctgttggtac ccccaggacc tgaaagcttc cgccttttta ctagagaatc
      841 tcttgctgct atcgaaaaac gtgctgcaga agagaaagcc aagaagccca aaaaggaaca
      901 agataatgat gatgagaaca aaccaaagcc aaatagtgac ttggaagctg gaaagaacct
      961 tccatttatt tatggagaca ttcctccaga gatggtgtca gagcccctgg aggacctgga
     1021 tccctactat atcaataaga aaacttttat agtaatgaat aaaggaaagg caattttccg
     1081 attcagtgcc acctctgcct tgtatatttt aactccacta aaccctgtta ggaaaattgc
     1141 tatcaagatt ttggtacatt ctttattcag catgcttatc atgtgcacta ttttgaccaa
     1201 ctgtgtattt atgaccttga gcaaccctcc tgactggaca aagaatgtag agtacacatt
     1261 cactggaatc tatacctttg agtcacttat aaaaatcttg gcaagagggt tttgcttaga
     1321 agattttacg tttcttcgtg atccatggaa ctggctggat ttcagtgtca ttgtgatggc
     1381 atatgtgaca gagtttgtgg acctgggcaa tgtctcagcg ttgagaacat tcagagttct
     1441 ccgagcactg aaaacaattt cagtcattcc aggtttaaag accattgtgg gggccctgat
     1501 ccagtcggta aagaagcttt ctgatgtgat gatcctgact gtgttctgtc tgagcgtgtt
     1561 tgctctcatt gggctgcagc tgttcatggg caatctgagg aataaatgtt tgcagtggcc
     1621 cccaagcgat tctgcttttg aaaccaacac cacttcctac tttaatggca caatggattc
     1681 aaatgggaca tttgttaatg taacaatgag cacatttaac tggaaggatt acattggaga
     1741 tgacagtcac ttttatgttt tggatgggca aaaagaccct ttactctgtg gaaatggctc
     1801 agatgcaggc cagtgtccag aaggatacat ctgtgtgaag gctggtcgaa accccaacta
     1861 tggctacaca agctttgaca cctttagctg ggctttcctg tctctatttc gactcatgac
     1921 tcaagactac tgggaaaatc tttaccagtt gacattacgt gctgctggga aaacatacat
     1981 gatatttttt gtcctggtca ttttcttggg ctcattttat ttggtgaatt tgatcctggc
     2041 tgtggtggcc atggcctatg aggagcagaa tcaggccacc ttggaagaag cagaacaaaa
     2101 agaggccgaa tttcagcaga tgctcgaaca gcttaaaaag caacaggaag aagctcaggc
     2161 agttgcggca gcatcagctg cttcaagaga tttcagtgga ataggtgggt taggagagct
     2221 gttggaaagt tcttcagaag catcaaagtt gagttccaaa agtgctaaag aatggaggaa
     2281 ccgaaggaag aaaagaagac agagagagca ccttgaagga aacaacaaag gagagagaga
     2341 cagctttccc aaatccgaat ctgaagacag cgtcaaaaga agcagcttcc ttttctccat
     2401 ggatggaaac agactgacca gtgacaaaaa attctgctcc cctcatcagt ctctcttgag
     2461 tatccgtggc tccctgtttt ccccaagacg caatagcaaa acaagcattt tcagtttcag
     2521 aggtcgggca aaggatgttg gatctgaaaa tgactttgct gatgatgaac acagcacatt
     2581 tgaagacagc gaaagcagga gagactcact gtttgtgccg cacagacatg gagagcgacg
     2641 caacagtaac gttagtcagg ccagtatgtc atccaggatg gtgccagggc ttccagcaaa
     2701 tgggaagatg cacagcactg tggattgcaa tggtgtggtt tccttggtgg gtggaccttc
     2761 agctctaacg tcacctactg gacaacttcc cccagagggc accaccacag aaacggaagt
     2821 cagaaagaga aggttaagct cttaccagat ttcaatggag atgctggagg attcctctgg
     2881 aaggcaaaga gccgtgagca tagccagcat tctgaccaac acaatggaag aacttgaaga
     2941 atctagacag aaatgtccgc catgctggta tagatttgcc aatgtgttct tgatctggga
     3001 ctgctgtgat gcatggttaa aagtaaaaca tcttgtgaat ttaattgtta tggatccatt
     3061 tgttgatctt gccatcacta tttgcattgt cttaaatacc ctctttatgg ccatggagca
     3121 ctaccccatg actgagcaat tcagtagtgt gttgactgta ggaaacctgg tctttactgg
     3181 gattttcaca gcagaaatgg ttctcaagat cattgccatg gatccttatt actatttcca
     3241 agaaggctgg aatatctttg atggaattat tgtcagcctc agtttaatgg agcttggtct
     3301 gtcaaatgtg gagggattgt ctgtactgcg atcattcaga ctgcttagag ttttcaagtt
     3361 ggcaaaatcc tggcccacac taaatatgct aattaagatc attggcaatt ctgtgggggc
     3421 tctaggaaac ctcaccttgg tgttggccat catcgtcttc atttttgctg tggtcggcat
     3481 gcagctcttt ggtaagagct acaaagaatg tgtctgcaag atcaatgatg actgtacgct
     3541 cccacggtgg cacatgaacg acttcttcca ctccttcctg attgtgttcc gcgtgctgtg
     3601 tggagagtgg atagagacca tgtgggactg tatggaggtc gctggccaaa ccatgtgcct
     3661 tattgttttc atgttggtca tggtcattgg aaaccttgtg gttctgaacc tctttctggc
     3721 cttattgttg agttcattta gctcagacaa ccttgctgct actgatgatg acaatgaaat
     3781 gaataatctg cagattgcag taggaagaat gcaaaaggga attgattatg tgaaaaataa
     3841 gatgcgggag tgtttccaaa aagccttttt tagaaagcca aaagttatag aaatccatga
     3901 aggcaataag atagacagct gcatgtccaa taatactgga attgaaataa gcaaagagct
     3961 taattatctt agagatggga atggaaccac cagtggtgta ggtactggaa gcagtgttga
     4021 aaaatacgta atcgatgaaa atgattatat gtcattcata aacaacccca gcctcaccgt
     4081 cacagtgcca attgctgttg gagagtctga ctttgaaaac ttaaatactg aagagttcag
     4141 cagtgagtca gaactagaag aaagcaaaga gaaattaaat gcaaccagct catctgaagg
     4201 aagcacagtt gatgttgttc taccccgaga aggtgaacaa gctgaaactg aacccgaaga
     4261 agaccttaaa ccggaagctt gttttactga aggatgtatt aaaaagtttc cattctgtca
     4321 agtaagtaca gaagaaggca aagggaagat ctggtggaat cttcgaaaaa cctgctacag
     4381 tattgttgag cacaactggt ttgagacttt cattgtgttc atgatccttc tcagtagtgg
     4441 tgcattggcc tttgaagata tatacattga acagcgaaag actatcaaaa ccatgctaga
     4501 atatgctgac aaagtcttta cctatatatt cattctggaa atgcttctca aatgggttgc
     4561 ttatggattt caaacatatt tcactaatgc ctggtgctgg ctagatttct tgatcgttga
     4621 tgtttctttg gttagcctgg tagccaatgc tcttggctac tcagaactcg gtgccatcaa
     4681 atcattacgg acattaagag ctttaagacc tctaagagcc ttatcccggt ttgaaggcat
     4741 gagggtggtt gtgaatgctc ttgttggagc aattccctct atcatgaatg tgctgttggt
     4801 ctgtctcatc ttctggttga tctttagcat catgggtgtg aatttgtttg ctggcaagtt
     4861 ctaccactgt gttaacatga caacgggtaa catgtttgac attagtgatg ttaacaattt
     4921 gagtgactgt caggctcttg gcaagcaagc tcggtggaaa aacgtgaaag taaactttga
     4981 taatgttggc gctggctatc ttgcactgct tcaagtggcc acatttaaag gctggatgga
     5041 tattatgtat gcagctgttg attcacgaga tgttaaactt cagcctgtat atgaagaaaa
     5101 tctgtacatg tatttatact ttgtcatctt tatcatcttt gggtcattct tcactctgaa
     5161 tctattcatt ggtgtcatca tagataactt caaccagcag aaaaagaagt ttggaggtca
     5221 agacatcttt atgacagagg aacagaaaaa atattacaat gcaatgaaga aacttggatc
     5281 caagaaacct cagaaaccca tacctcgccc agcaaacaaa ttccaaggaa tggtctttga
     5341 ttttgtaacc agacaagtct ttgatatcag catcatgatc ctcatctgcc tcaacatggt
     5401 caccatgatg gtggaaacgg atgaccaggg caaatacatg accctagttt tgtcccggat
     5461 caacctagtg ttcattgttc tgttcactgg agaatttgtg ctgaagctcg tctccctcag
     5521 acactactac ttcactatag gctggaacat ctttgacttt gtggtggtga ttctctccat
     5581 tgtaggtatg tttctggctg agatgataga aaagtatttt gtgtccccta ccttgttccg
     5641 agtgatccgt cttgccagga ttggccgaat cctacgtctg atcaaaggag caaaggggat
     5701 ccgcacgctg ctctttgctt tgatgatgtc ccttcctgcg ttgtttaaca tcggcctcct
     5761 gctcttcctg gtcatgttta tctatgccat ctttgggatg tccaactttg cctatgttaa
     5821 aaaggaagct ggaattgatg acatgttcaa ctttgagacc tttggcaaca gcatgatctg
     5881 cttgttccaa attacaacct ctgctggctg ggatggattg ctagcaccta ttcttaatag
     5941 tgcaccaccc gactgtgacc ctgacacaat tcaccctggc agctcagtta agggagactg
     6001 tgggaaccca tctgttggga ttttcttttt tgtcagttac atcatcatat ccttcctggt
     6061 tgtggtgaac atgtacatcg cggtcatcct ggagaacttc agtgttgcta ctgaagaaag
     6121 tgcagagccc ctgagtgagg atgactttga gatgttctat gaggtttggg aaaagtttga
     6181 tcccgatgcg acccagttta tagagttctc taaactctct gattttgcag ctgccctgga
     6241 tcctcctctt ctcatagcaa aacccaacaa agtccagctt attgccatgg atctgcccat
     6301 ggtcagtggt gaccggatcc actgtcttga tattttattt gcctttacaa agcgtgtttt
     6361 gggtgagagt ggagagatgg atgcccttcg aatacagatg gaagacaggt ttatggcatc
     6421 aaacccctcc aaagtctctt atgagcctat tacaaccact ttgaaacgta aacaagagga
     6481 ggtgtctgcc gctatcattc agcgtaattt cagatgttat cttttaaagc aaaggttaaa
     6541 aaatatatca agtaactata acaaagaggc aattaaaggg aggattgact tacctataaa
     6601 acaagacatg attattgaca aactaaatgg gaactccact ccagaaaaaa cagatgggag
     6661 ttcctctacc acctctcctc cttcctatga tagtgtaaca aaaccagaca aggaaaagtt
     6721 tgagaaagac aaaccagaaa aagaaagcaa aggaaaagag gtcagagaaa atcaaaagct
     6781 cgaggactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga
     6841 cgatgacaag tagtgagggc ccgatatctc gacaatcaac ctctggatta caaaatttgt
     6901 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6961 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     7021 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     7081 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7141 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7201 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7261 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     7321 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7381 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7441 tccctttggg ccgcctcccc gcctggaaac gggggaggct aactgaaaca cggaaggaga
     7501 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt
     7561 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     7621 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     7681 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     7741 gcagatctgc gcagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     7801 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7861 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7921 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7981 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8041 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8101 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc
     8161 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     8221 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     8281 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     8341 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     8401 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8461 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     8521 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     8581 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     8641 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     8701 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     8761 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     8821 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     8881 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     8941 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     9001 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     9061 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     9121 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     9181 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     9241 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     9301 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     9361 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     9421 ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     9481 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     9541 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     9601 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9661 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     9721 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     9781 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     9841 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     9901 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     9961 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    10021 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    10081 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10141 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    10201 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    10261 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    10321 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
    10381 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    10441 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    10501 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    10561 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
    10621 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    10681 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    10741 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    10801 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    10861 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10921 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    10981 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    11041 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    11101 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    11161 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    11221 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    11281 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    11341 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    11401 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    11461 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    11521 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    11581 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    11641 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    11701 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    11761 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    11821 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    11881 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
    11941 tatggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
    12001 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
    12061 aaggcttgac cgacaattgc atgaagaatc tgcttagg
//
