LOCUS V031126 6249 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031126 VERSION V031126 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..2396 /label="Ruvbl1(NM_019685)" /note="Ruvbl1(NM_019685)" /gene="Ruvbl1" CDS 2415..2444 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 2463..2486 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(2506..2522) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 2533..3155 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(3304..3892) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 4221..4268 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(4500..5294) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(5329..5686) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5688..5792) /gene="bla" /label="AmpR promoter" rep_origin 5819..6247 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gaagattgag gaggtgaaga gcaccacgaa gacgcagcgc atcgcctccc 1081 acagccacgt gaagggtctg gggctggacg agagcggcct agccaagcag gcggcttcgg 1141 gtctcgtggg ccaggagaac gcgagagagg catgtggcgt cattgtagaa ttaatcaaaa 1201 gcaagaaaat ggctggaaga gctgtcttgt tggcggggcc tcctggaact ggcaagacag 1261 ccttggccct tgctattgct caggagctgg gcagtaaagt ccctttctgc ccgatggtgg 1321 gtagcgaggt atactcaact gagatcaaaa agacagaggt gctgatggag aacttccgca 1381 gggccattgg gttgcggata aaggagacta aggaggttta tgaaggggag gtgacagagc 1441 tcactccctg tgagacagag aaccccatgg gtgggtacgg caaaactatc agccatgtga 1501 tcatagggct caagactgct aaaggaacca aacagctgaa gctggacccc agtatttttg 1561 aaagtttgca gaaagaacga gtagaggctg gagatgtgat ttacattgaa gcaaatagtg 1621 gagctgtgaa gaggcaaggc aggtgtgaca cctatgccac agagtttgac cttgaagctg 1681 aagagtacgt ccctttgcca aagggagatg tgcacaagaa gaaggaaatc atacaggatg 1741 tgaccttgca tgacctggat gtggctaatg cgcggcctca gggtgggcaa gatattctgt 1801 ctatgatggg ccagttgatg aagccaaaaa agacagagat cacagataaa cttcgagggg 1861 agatcaacaa agtggtgaac aaatacattg accagggtgt ggccgagctg gtccctggag 1921 tgctttttgt cgatgaggtc cacatgctgg atatcgagtg ctttacctac ctgcaccgag 1981 ccctggagtc ctccatcgcc cccattgtca tctttgcatc caaccgaggc aactgtgtca 2041 tcaggggcac tgaggacatc acttctccac acggcatccc gttggatctg ctagacaggg 2101 tgatgatcat caggaccatg ctatacacgc cacaggagat gaagcagatc attaagatcc 2161 gagcccagac ggaaggcatc aacatcagcg aggaggcgct gaaccacctc ggggagattg 2221 gcaccaagac cactctgagg tattcggtgc agctgctgac cccagccaac ctgctggcaa 2281 agatcaatgg caaggacagc attgagaagg agcacgtgga agagatcagc gagctcttct 2341 atgacgccaa gtcctctgcc aagattttgg ctgaccagca ggacaagtac atgaagacgc 2401 gtacgcggcc gctcgagcag aaactcatct cagaagagga tctggcagca aatgatatcc 2461 tggattacaa ggatgacgac gataaggttt aaacggccgg ccgcggtcat agctgtttcc 2521 tgaacagatc ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa 2581 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct 2641 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa 2701 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg 2761 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag 2821 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt 2881 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg 2941 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc 3001 cctgtccttc tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg 3061 ctacctggcc atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca 3121 tgcgttgggt ccactcagta gatgcctgtt gaattgggta cgcggccagc ggcgagcggt 3181 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 3241 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 3301 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 3361 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 3421 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 3481 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 3541 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 3601 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 3661 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 3721 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 3781 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 3841 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 3901 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 3961 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 4021 taaatcaatc taaagtatat atgagtaacc tgaggctatg gcagggcctg ccgccccgac 4081 gttggctgcg agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga 4141 aacgcgggcg tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg 4201 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccg tgcgttttat tctgtctttt 4261 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct 4321 ccccctaggg tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc 4381 ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa 4441 atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct 4501 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata 4561 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg 4621 gtagccaacg ctatgtcctg atagcgatcc gccacaccca gccggccaca gtcgatgaat 4681 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg 4741 acgagatcct cgccgtcggg catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg 4801 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta 4861 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc 4921 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga 4981 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca 5041 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc 5101 gctgcctcgt cttgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc 5161 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt 5221 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca 5281 tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcgatctt tgcaaaagcc 5341 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc 5401 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg 5461 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag 5521 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc 5581 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc 5641 acaccctaac tgacacacat tccacagctg gttctttccg cctcaggact cttccttttt 5701 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 5761 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 5821 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 5881 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 5941 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 6001 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 6061 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 6121 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 6181 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 6241 gcgaatttt //