LOCUS       V026963                 6188 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026963
VERSION     V026963
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1055..2350
                     /label="RNF135(NM_032322)"
                     /note="RNF135(NM_032322)"
                     /gene="RNF135"
     CDS             2354..2383
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2402..2425
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2445..2461)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2472..3094
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3243..3831)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4160..4207
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4439..5233)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5268..5625)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5627..5731)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5758..6186
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgcggc gcgccagatc tcaagcttgc caccatggcg ggcctgggcc tgggctccgc
     1081 cgttcccgtg tggctggccg aggacgacct cggctgcatc atctgccagg ggctgctgga
     1141 ctggcccgcc acgctgccct gcggccacag cttctgccgc cactgcctgg aggccctgtg
     1201 gggcgcccgc gacgcccgcc gctgggcctg ccccacttgc cgccagggcg ccgcgcagca
     1261 gccgcacctg cggaagaaca cgctactgca ggacctggcc gacaagtacc gccgcgccgc
     1321 acgcgagata caggcgggct ccgaccctgc ccactgcccc tgcccgggct ccagttccct
     1381 ctccagcgcg gccgcgaggc cccggcgccg cccggaactg cagcgggtgg cagtagagaa
     1441 gagcatcaca gaagttgctc aggagctgac agagctggtg gaacatcttg tagacattgt
     1501 cagaagcctg cagaatcaga ggcccctatc agaatctgga ccagacaacg aactgagcat
     1561 cctgggcaag gctttttctt ctggggtgga tctttccatg gcttctccaa agctggtgac
     1621 ttccgacaca gctgcaggga aaatcagaga tattctccat gacctagaag aaattcagga
     1681 aaaattacaa gaaagcgtca cctggaaaga ggctcctgaa gcacaaatgc agggagaact
     1741 cctggaagcc ccgtcttcct cctcatgccc attgcctgac cagagccacc ctgcactcag
     1801 gagagcttct cggtttgctc agtgggccat ccatccaacc tttaacttga agagcctttc
     1861 ctgcagcctg gaggtgtcca aggattcccg tacagtgact gtgtctcacc gcccacaacc
     1921 ctatcgctgg agctgtgaga ggttttctac cagccaggtc ttatgttccc aggccctgtc
     1981 ttctggaaag cattactggg aagtggacac taggaattgc agccactggg cagttggggt
     2041 ggcttcctgg gagatgagcc gcgaccaggt cctgggaagg actatggact cttgttgtgt
     2101 ggaatggaag gggactagcc agctctctgc atggcacatg gtcaaggaaa ctgtccttgg
     2161 ctcagacaga cctggggtgg tgggcatctg gctgaacctt gaggagggaa agcttgcctt
     2221 ctattcagtg gacaatcagg agaagcttct gtatgagtgt accatctctg cctcctctcc
     2281 tttgtaccct gccttctggc tgtatggctt acatcctgga aattacctga taataaagca
     2341 agtaaaggtg ctcgagcaga aactcatctc agaagaggat ctggcagcaa atgatatcct
     2401 ggattacaag gatgacgacg ataaggttta aacggccggc cgcggtcata gctgtttcct
     2461 gaacagatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag
     2521 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
     2581 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag
     2641 ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg
     2701 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc
     2761 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt
     2821 ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga
     2881 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc
     2941 ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca cagcataggc
     3001 tacctggcca tgcccaaccg gtgggacatt tgagttgctt gcttggcact gtcctctcat
     3061 gcgttgggtc cactcagtag atgcctgttg aattgggtac gcggccagcg gcgagcggta
     3121 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     3181 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3241 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3301 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3361 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3421 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3481 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3541 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3601 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3661 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3721 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3781 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3841 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3901 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3961 aaatcaatct aaagtatata tgagtaacct gaggctatgg cagggcctgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttggggggt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggcccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt
     4201 attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc agttagcctc
     4261 cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg
     4321 gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa
     4381 tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc
     4441 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
     4501 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
     4561 tagccaacgc tatgtcctga tagcgatccg ccacacccag ccggccacag tcgatgaatc
     4621 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
     4681 cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg gctggcgcga
     4741 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
     4801 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
     4861 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
     4921 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
     4981 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
     5041 ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
     5101 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
     5161 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
     5221 cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcgatcttt gcaaaagcct
     5281 aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg
     5341 gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc
     5401 ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga
     5461 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct
     5521 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
     5581 caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc ttcctttttc
     5641 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5701 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     5761 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5821 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5881 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5941 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6001 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6061 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6121 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6181 cgaatttt
//
