LOCUS V041544 7192 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V041544 VERSION V041544 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..1851 /label="Ptpn2(NM_008977)" /note="Ptpn2(NM_008977)" /gene="Ptpn2" CDS 1858..1884 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1888..1914 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1918..1944 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 1968..2556 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2622..2670 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 2872..3300 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3314..3643 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3710..4504 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4680..4801 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4850..4866) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4874..4890 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4898..4928) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4943..4964 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5252..5840) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6011..6871) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6872..6976) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgtc ggcaaccatc 721 gagcgggagt tcgaggaact ggatgctcag tgtcgctggc agccgttata cttggaaatt 781 cgaaatgaat cccatgacta tcctcataga gtggccaagt ttccagaaaa cagaaaccga 841 aacagataca gagatgtaag cccatatgat cacagtcgtg ttaaactgca aagtactgaa 901 aatgattata ttaatgccag cttagttgac atagaagagg cacaaagaag ttacatctta 961 acacagggcc cacttccgaa cacatgctgc catttctggc tcatggtgtg gcagcaaaag 1021 accaaagcag ttgtcatgct aaaccgaact gtagaaaaag aatcggttaa atgtgcacag 1081 tactggccaa cggatgacag agaaatggtg tttaaggaaa cgggattcag tgtgaagctc 1141 ttatctgaag atgtaaaatc atattataca gtacatctac tacagttaga aaatatcaat 1201 actggtgaaa ccagaaccat atctcacttc cattatacca cctggccaga ttttggggtt 1261 ccagagtcac cagcttcatt tctaaacttc ttgtttaaag ttagagaatc tggttgtttg 1321 acccctgacc atggacctgc agtgatccat tgcagtgcgg gcatcgggcg ctctggcacc 1381 ttctctcttg tagatacctg tcttgttctg atggaaaaag gagaggatgt taatgtgaaa 1441 caattattac tgaatatgag aaagtatcga atgggactta ttcagacacc ggaccaactc 1501 agattctcct acatggccat aatagaagga gcaaagtaca caaaaggaga ttcaaatata 1561 cagaaacggt ggaaagaact ttctaaagaa gatttatctc ctatttgtga tcattcacag 1621 aacagagtga tggttgagaa gtacaatggg aagagaatag gttcagaaga tgaaaagtta 1681 acagggcttc cttctaaggt gcaggatact gtggaggaga gcagtgagag cattctacgg 1741 aaacgtattc gagaggatag aaaggctacg acggctcaga aggtgcagca gatgaaacag 1801 aggctaaatg aaactgaacg aaaaagaaaa aggccaagat tgacagacac cctcgagtac 1861 ccatacgacg tacctgatta tgcaacttat ccttatgatg tacctgatta tgctagctat 1921 ccttatgatg tcccagacta cgcatagtga gggcccgata tctcgacaat caacctctgg 1981 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat 2041 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt 2101 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca 2161 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg 2221 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 2281 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca 2341 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca 2401 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc 2461 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc 2521 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aaacggggga ggctaactga 2581 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa 2641 taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc 2701 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt 2761 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg 2821 caggccctgc catagcagat ctgcgcagct ggggctctag ggggtatccc cacgcgccct 2881 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 2941 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 3001 gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac 3061 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 3121 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 3181 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 3241 tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 3301 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 3361 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 3421 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 3481 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 3541 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 3601 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 3661 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 3721 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 3781 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 3841 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 3901 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 3961 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 4021 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 4081 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 4141 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 4201 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 4261 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 4321 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 4381 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 4441 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 4501 ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac 4561 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 4621 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 4681 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 4741 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 4801 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 4861 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 4921 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 4981 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 5041 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 5101 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 5161 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 5221 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 5281 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 5341 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 5401 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta 5461 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 5521 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 5581 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 5641 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 5701 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 5761 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 5821 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 5881 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 5941 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 6001 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 6061 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 6121 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 6181 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 6241 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 6301 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 6361 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 6421 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 6481 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 6541 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 6601 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 6661 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 6721 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 6781 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 6841 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 6901 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 6961 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag 7021 atctcccgat cccctatggt cgactctcag tacaatctgc tctgatgccg catagttaag 7081 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta 7141 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gg //